Objective To know the master condition about sexual knowledge in female workers in Dongguan, and then carry out certain effective community nursing intervention to populize the related knowledge in female workers. Methods Divided 3000 femle workers with 18-35 years old who had selected in 9 factories in Dongguan into the DV group and the teacher education group randomly, there were 1500 cases in each group. Video displaied was used in the DV group in certain propert time, teacher education about sexual knowledge was used in the teacher education group, compared the master condi-tion of sexual knowledge before and after the interventioni by questionnair in the two groups respectively, and then summarized the results. Results There were no significant differences between the two groups about sexual knowledge before the intervention, while after the intervention, the master conditon of sexual knowl-edge in the DV group were better than those of in the teacher education group. Conclusions Video dis-play is a kind of saving, effectiveness, open-end and circular mode for female workers to pepulize sexual-related knowledge, which should be ranging used.