Objective To construct h n bone marrow mesenchymal stem cell line genetically modified with human proenkephalin gene. Methods The packaging cell line Phoenix-293T was transfected with the recombinant pBABE-PENK vector to aquire virus. The recombinant virus was then collected and used to infect hMSCs. Stable expression of proenkephalin gene and leucine enkephalin protein and the concentration of leucine enkephalin protein were detected by RT-PCR, immunofluorescence and ELISA respectively. Results The expression of proenkephalin gene and leucine enkephalin protein were significantly up-regulated in the hMSC-PENK cells, and the concentration of leucine enkephalin protein was also increased in the culture medium. Conclusion A human mesenchymal stem cell line that expresses proenkephalin gene and secrets enkephalin was successfully established.

In order to meet the modern needs in medical talent education and bring up highly qualitfied medical talent,Medical College of Shantou University has aggressively and effectively conducted exploration and practice on high medical education,and developed a new teaching model for bringing up medical talent under with high quality the strategies of "Reform of Course System" and "Innovation of Teaching Model".The new teaching model is designed to bring up excellent clinicians with innovative spirit and proficient basic skills.It keeps up with the global ideal of high medical education,and it is an originated teaching model in China with Chinese characteristics and a unique style of Medical College of Shantou University,which will make a positive and significant impact on Chinese high medical education.

Objective To explore the effect on immunologic function of gastric carcinoma patients after operation by transposition of a transverse colon segment as a gastric reservoir following total gastrectomy.Methods A prospective,randomized,double-blind clinical trial was performed.One hundred and sixty-seven gastric carcinoma patients were divided into control group and research group in terms of balanced random groups.We reconstructed digestive tract with conventional Schlatter esophagojejunostomy and Roux-enY esophagojejunostomy in control group,while we reconstructed digestive tract with transposition of a transverse colon segment as a gastric reservoir in rcsearch group.the changes of T cell subset,IL-2,immunologic function of red blood cell,acute inflammatory mediator were analyzed in the patients respectively at the first day before operation,at the first day after operation,at the ninth day after operation.Postoperative fatality rate was also detected in two groups.Results Compared with control group,The changes of T cell subset,IL-2 had no significant differences in research group at the first day after operation (P ＞ 0.05).chaplet Meanwhile,the changes of C3b receptor chaplet rate and immunocomplex receptor rate had no significant differences in rcscarch group compared with control group at the first day after operation (P ＞ 0.05).There were no obvious differences for acute inflammatory mediators between two groups at the first day after operation (P＞0.05).At the ninth day after operation,the levels of CD4+ (44.68 ±5.92)％ in control group were obviously lower than those (48.75 ± 6.24)％ in research group (P ＜ 0.05).However,the percentage of CD8 + T cells (27.21 ± 3.07) ％ in control group was significantly higher than that (24.26 ±2.39) ％ in research group (P ＜ 0.05).The levels of IL-2 in control group and research group were (1.49 ± 0.32) μg/L and (1.67 ± 0.35) μg/L,with obvious differences between two groups (P ＜ 0.05).At the ninth day after operation,C3b receptor chaplet rate (39.27 ± 6.88) ％ in research group was markedly higher than that (30.25 ± 6.65) ％ in control group (P ＜ 0.05).The levels of IL-6 in control group were (125.51 ± 18.24) pg/mL,while the levels of IL-6 in research group were(87.34 ± 12.88) pg/mL,with obvious differences (P ＜ 0.05).There was no significant difference for postoperative fatality rate between two groups (P ＞ 0.05).Conclusions The transposition of a transverse colon segment as a gastric reservoir is a safely new mode in the reconstruction of alimentary.It is positive to improve immunologic function for gastric carcinoma patients after operation.

OBJECTIVE: To explore the influence of hypoxemia on the hearing of children with childhood obstructive sleep apnea-hypopnea syndrome (OSAHS). METHOD: Auditory brainstem response (ABR) and distortion-product otoacoustic emission (DPOAE) was recorded in 68 ears and 60 ears respectively of children suffering from OSAHS with "A" tympanogram. Meanwhile, ABR and DPOAE was also recorded in 30 controls of children with "A" tympanogram. RESULT: There was no statistical difference between the mild OSAHS group and the control group in the latency of wave I, III and V, the interval between wave I and III, III and V, I and V. There was significant difference between the moderate and severe OSAHS group and the control group in the delayed latency of wave I. There was significant difference between the mild OSAHS group and the control group in the amplitudes of DPOAE at 8 kHz. There was significant difference between the moderate and severe OSAHS group and the control group in the amplitudes of DPOAE at 6 kHz and 8 kHz. CONCLUSION Cochlear function was affected when AHI > or = 10/h. ABR and DPOAE could be used to detect the early damagement of auditory function in childhood OSAHS.
Adolescent ; Child ; Child, Preschool ; Evoked Potentials, Auditory, Brain Stem ; Female ; Humans ; Male ; Otoacoustic Emissions, Spontaneous ; Sleep Apnea, Obstructive ; physiopathology

Objective To investigate the risk factors of neonatal hypoglycemia,establish the risk prediction model of neonatal hypoglycemia and test the effectiveness of the model.Methods Retrospective analysis was performed to collect clinical data of 727 newborns and pregnant mothers who were delivered in a Grade Ⅲ general hospital from October 2018 to August 2020.Univariate analysis and multivariate Logistic regression analysis were used to analyze related risk factors to construct prediction models.The clinical data of 150 newborns and pregnant women from September 2020 to February 2021 were selected to test the efficacy of the model.Results Multivariate Logistic regression analysis showed that feeding problems,neonatal hypothermia,neonatal complications,gestational diabetes and fetal distress were independent risk factors for neonatal hypoglycemia(P<0.05).The model verification results showed that the area under the curve(AUC)was 0.883,the sensitivity was 82.97%,the specificity was 88.35%,the positive predictive value was 76.47%,the negative predictive value was 91.92%,and the total accuracy of the model was 88.67%,which had a good prediction ability.Conclusion The prediction model established in this study has a good ability to predict the risk of neonatal hypoglycemia,which can be used to provide reference for early screening of high-risk groups of neonatal hypoglycemia and starting predictive nursing intervention measures.

Venous thromboembolism (VTE) is a complication in children with acute lymphoblastic leukemia (ALL). The Chinese Children's Cancer Group-ALL-2015 protocol was carried out in China, and epidemiology, clinical characteristics, and risk factors associated with VTE were analyzed. We collected data on VTE in a multi-institutional clinical study of 7640 patients with ALL diagnosed in 20 hospitals from January 2015 to December 2019. First, VTE occurred in 159 (2.08%) patients, including 90 (56.6%) during induction therapy and 108 (67.92%) in the upper extremities. T-ALL had a 1.74-fold increased risk of VTE (95% CI 1.08-2.8, P = 0.022). Septicemia, as an adverse event of ALL treatment, can significantly promote the occurrence of VTE (P < 0.001). Catheter-related thrombosis (CRT) accounted for 75.47% (n = 120); and, symptomatic VTE, 58.49% (n = 93), which was more common in patients aged 12-18 years (P = 0.023), non-CRT patients (P < 0.001), or patients with cerebral thrombosis (P < 0.001). Of the patients with VTE treated with anticoagulation therapy (n = 147), 4.08% (n = 6) had bleeding. The VTE recurrence rate was 5.03% (n = 8). Patients with VTE treated by non-ultrasound-guided venous cannulation (P = 0.02), with residual thrombus (P = 0.006), or with short anticoagulation period (P = 0.026) had high recurrence rates. Thus, preventing repeated venous puncture and appropriately prolonged anticoagulation time can reduce the risk of VTE recurrence.
Humans ; Child ; Venous Thromboembolism/etiology* ; East Asian People ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/epidemiology* ; Risk Factors ; Thrombosis/chemically induced* ; China/epidemiology* ; Anticoagulants/adverse effects* ; Recurrence