0.05. Statistics from 7 papers revealed the mean survival rate of TCM group was 335. 4 days, that of chemotherapeutic group 231. 8, test of significance P = 0. 1489. MA results showed that the features of TCM treatment on NSCL were: high stability, low effectiveness, however, with certain dominance. Authors' address: Institute of Oncology, Shijiazhuang, Hebei 050011

Objective To compare the pathogenesis of coronary heart disease (CHD) with heart_blood stagnation syndrome (HBSS) due to Qi deficiency and Qi stagnation. Methods Indices such as endothelin,NO,TXB 2 ,6-Keto-PGF 1? ,blood rheology,platelet aggregation rate,atrial natriuretic factor,high_frequency electrocardiogram and cardiac function were observed. Results The changes of the above indices in CHD with HBSS due to Qi stagnation (Group A)were smaller than those due to Qi deficiency(Group B). Conclusion Qi stagnation is the primary stage of CHS with HBSS,the pathological changes being mild;Qi deficiency is the advanced stage,the pathological changes being severe.

Objective Calcified epithelioma is a rare benign tumor in head and face. It is seldomly seen canceration. But it has local invasion behavior. The aim of this study was to explore the methodology in the diagnosis and treatment of calcified epithelioma in head and face, and to improve the accuracy of clinical diagnosis. Methods A retrospective analysis was carried out in 26 patients with calcified epithelioma in head and face. Results 53. 8 % patients with calcified epithelioma were less than 13-year-old. The disease was easily misdiagnozed. In this group of patients the misdiagnosis rate reached to 42. 3 %. Btype ultrasonographic diagnosis possessed a high level of concordance with histological diagnosis. There were three major types of calcified epithelioma: type 1, a mass with internal echoic foci; type 2, a hyperechoic mass with high beam; type 3, no calcification in the parenchyma. Piomatricoma was described as a skin tumour histologically composed of shadowcells, basophillic cells, foreign body giant cells and intracellular and stromal calcification. The treatment was excision and no recurrences were found by followup. Conclusions It is important for clinicians to know the clinical features of calcified epithelioma. Imaging studies including ultrasonography would help establish the diagnosis. Surgical excision with clear margins is recommended for this tumor.

【Objective】To observe the in-vitro effect of panax notoginseng saponins(PNS) on bone marrow mesenchymal stem cells(MSCs) proliferation and on inducing MSCs to differentiate into cardiomyogenic cells.【Methods】MSCs were isolated from adult SD rats,and then were cultured and cloned by density gradient method and adhesive cultivation.The cell surface markers of MSCs were detected with flow cytometer.The effect of PNS on MSCs proliferation was observed with methyl thiazolyl tetrazolium(MTT) assay.The 8th generation of continuous cultured MSCs was used to observe the in-vitro differentiation of cardiomyogenic cells.The cardiomyogenic cells were identified under phase contrast microscope by immunohistochemical method and reverse transcription-polymerase chain reaction(RT-PCR) analysis.【Results】CD44 expression of MSCs was positive while that of CD34 expression of bone marrow hematopoietic stem cells was negative,indicating the success of the MSCs culture.After induction with PNS,the increase of MSCs was obvious as compared with the blank control group,and there existed histological changes of MSCs after in-vitro induction.The expression of specific proteins of Desmin and cardiac troponin I(cTnI) in MSCs was positive,and the results of RT-PCR analysis showed that the expression of myosin heavy chain(MHC) was increased.【Conclusion】PNS can increase the in-vitro proliferation of MSCs and induce MSCs to differentiate into cardiomyogenic cells in vitro,and this will supply experimental evidence of cell transplantation for the treatment of myocardial infarction.

Objective To build a digitized 3D model of the pulmonary venous system of Chinese people for the purpose of providing anatomic information of the pulmonary venous system for anatomic teaching,imaging diagnosis and surgical operation of pulmonary diseases. Methods The primal images from the first Chinese visible human data set of chest were converted into image sequence only cantaining the sections of pulmonary veins after manual registration and image segmentation.Then these images were imported into 3DMed software to build a 3D model of the pulmonary venous system through threshold registration algorithm. Results The three dimensional structures of the pulmonary venous system were reconstructed entirely.All reconstructed structures could be displayed individually or jointly,and could be rotated continuously on any plane.Conclusion The branches and the spacial characters of the pulmonary venous system can be clearly shown on the reconstructed 3D model.It will help anatomy teaching and provide morphological data for image diagnosis and lung operations.

Objective To investigate the effect of Tianma Gouteng Decoction on myocardial fibrosis in renovascular hypertensive rat (RHR). Methods RHR models were established by occlusion of renal artery with artery forceps. The collagen concentration and the contents of collagen type I,III were measured. Meanwhile, the weight of heart and left ventricle was determined. Results (1)Both Tianma Gouteng Decoction and Captopril could reduce the left ventricle weight index (LVI) and collagen contents (P

[Objective] To observe the in-vitro inductive effect of ginsenosides (GS) on differentiation of rat bone marrow mesenchymal stem cells (MSC) into myocardial cells. [Methods] Marrow stromal cells were isolated from adult SD rats, and then were cultured and cloned by density gradient method and adhesive cultivation to prepare the primary MSC. The cell surface antigens of CD34 and CD44 were detected with flow cytometer to identify MSC. After subculture, the 8th generation of continuous MSC were induced by GS (250 mg/L) and 5-aza (5-azacytidine, 10 ?mol/L) and GS + 5-aza (250mg/L and 10 ?mol/L respectively) for 24, 48 and 72 hours to differentiate to myogenic cells. Meanwhile, a blank control group was set to compare the effect. After the induction, myocardial cell percentage was worked out after examining MSC count and positive cells count under the phase contrast microscope. The specific proteins of Desmin and cardiac troponin I (cTnI) in myocardial cells were detected by immunohistochemistry method, and the cardiac-specific gene expression of ?-MHC (myosin heavy chain) and ?-MHC in myocardiocytes was examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. [Results] The cultured marrow stromal cells grew well, spindle in shape, and their CD44 expression was positive, indicating that marrow stromal cells differentiated to MSC. After induction with GS and 5-aza, MSC differentiated to myocardial cells, and the myocardial cell percentage was 38% in GS group, 35% in 5-aza group and 39% in GS + 5-aza group, the difference being insignificant. The expression of Desmin, cTnI and MHC in MSC was positive, and MSC were spindle-shaped and looked like fibroblast, indicating that MSC differentiated to myocardial cells. [Conclusion] Ginsenosides can induce MSC to differentiate to myogenic cells in-vitro, and this will supply evidence for cell transplantation.

Objective To provide morphological data for image diagnosis and mediastinal surgery planning.Methods Cross-sectional images of fresh tissues from the first Chinese visible female human data-set were reviewed and the main structures of the superior mediastinum were confirmed on a section-by-section basis.The positional rule of the main structures in various plane of superior mediastinum was found out,and a few key sectional images were selected and compared with the relative images of CT or MRI,and the relative data on the cross-sectional images were collected.Results The cross-sectional images of the first Chinese visible female human fairly displayed the aortic arch and its 3 large branches,superior vena cava,left and right brachiocephalic veins,azygos vein,pulmonary trunk,trachea,esophagus,thoracic duct and so on,as well as their relationship clearly.Conclusion The first Chinese visible female human dataset can almost provide complete and accurate data.

Objective To find out the best orientation and section for diagnosing rheumatic heart disease using multiplane transesophageal echocardiography (TEE). Methods The visible heart was compared with the images by multiplane TEE for the establishment of the best orientation and section for multiplane TEE for the diagnosis of rheumatic heart disease. Results The best orientation and section of mitral valve detected by multiplane TEE were the four chamber view at 0? orientation at the end part of esophagus. The best orientation and section of aorta valve were short axis section of the heart at 45? orientation and long axis section of the heart at 135? orientation at the middle part of the esophagus. The best orientation and section were the short axis section at 45? orientation and the oblique short axis section at 0? orientation of the aorta valve at the middle and the upper part of esophagus. Conclusion The best orientation and section of TEE based on the study of the visible heart are helpful to simplify the operation procedure of multiplane TEE and to shorten the examining time.