Objective To explore the clinical curative effect of percutaneous coronary intervention ( PCI) for acute myocardial infarc-tion ( AMI) . Methods Retrospectively analyzed the clinical data of 138 patients with acute myocardial infarction who were admitted into our hospital from September 2012 to September 2014. The 138 patients were divided into the intravenous thrombolysis group (65 cases) and the PCI group (73 cases) according to the different way of treatment. And the basic clinical data, surgical outcomes, complications, and postop-erative follow-up results of the two groups were compared and analyzed. Results Reperfusion rate of related blood vessels,rate of TIMI flow reached grade 3, and score of left ventricular ejection fraction ( LVEF) in the PCI group were significantly higher than that of intravenous thrombolysis group, and the difference is statistically significant (P<0. 05). Degree of residual stenosis in the PCI group were significantly lower than that of intravenous thrombolysis group, and the difference is statistically significant (P<0. 05). Cacoethic ST-segment resolution and average hospital stay of the two groups had no significant difference (P>0. 05). Compared with the intravenous thrombolysis group, the curative effect of the PCI group was obviously better than the intravenous thrombolysis group with a statistically significant difference (P<0. 05). During the follow-up period, arrhythmia, angina, recurrence of myocardial infarction, and mortality within 6 months in the PCI group were all lower than in the intravenous thrombolysis group, and the difference is statistically significant (P<0. 05). Conclusion Per-cutaneous coronary interention for patients with acute myocardial infarction can effectively open the lesion vessels and relieve myocardial nec-rosis in patients, and it is of good curative effect.

Objective To explore the effect of transradial approach in percutaneous coronary intervention for elderly patient with coronary artery disease,and provide reference for clinic. Methods There were 198 patients older than 70 years with coronary artery disease in our hospital from March 2007 to March 2013,who were divided into the radial group(n=100) and the femoral group(n=98) according to differ-ent puncture pathways. The X-ray exposure time,operative time,amount of contrast medium,the success rate of puncture,achievement ratio of operation and venous complications were compared and analyzed. Results The X-ray exposure time,operative time,amount of contrast me-dium,the success rate of puncture,achievement ratio of operation of 2 groups has no significant differences(P>0. 05). The venous complica-tion of radial group was less than that of femoral group,the difference was statistical significance(P<0. 05). Conclusion The transradial approach in percutaneous coronary intervention for elderly patient with coronary artery disease was feasible and safe,with less complication.

Objective To explore the risk factors of hypotension of patient with acute myocardial infraction(AMI)after emergency per-cutaneous coronary intervention(PCI).Methods The data of 300 patients with AMI who received PCI in our hospital from January 2015 to August 2015 were collected.According to the symptom of hypotension,the patients were divided into hypotension group and normal group.The general information and relative clinical data were recorded and compared.The risk factors were analyzed by logistics regressive analysis. Results The incidence of previous angina of hypotension group was lower than that of normal group,the difference was significant(P <0.05), there was no difference in other general information between two groups(P >0.05).There was no difference in level of LDL-C between two groups(P >0.05).And the differences in peak value of CK-MB,inferior wall or right ventricular infarction were statistical significance (P <0.05).There was no significant difference in multi-vessel lesions(P >0.05),and the difference in others of coronary artery lesion be-tween two groups was statistical significance(P <0.05).Conclusion The peak value of CK-MB,inferior wall or right ventricular infarction and TIMI <2 after PCI can increase the possibility of hypotension after PCI,and angina pectoris before infarction can reduce the incidence of hypotension after PCI.

An in vitro model was built for researching the effects of strain on vascular smooth muscle cells (VSMCs). The cultured VSMCs was stretched by four-support-bending-beam system, then the project area of cells was measured by computer-image-processing, the adhesion force was measured by micropipette-aspirating system, the α-actin of VSMCs was distinguished by immunocytochemistry and the dynamic of VSMCs was determined by FCM. The results show that: (1) The adhesion force of VSMCs is positively related to time. The adhesion force of unit area is indistinct after stretched for four hour. (2) The amount of α-actin increases with stretching time. (3) The proliferation of VSMCs is a little inhibited by stretched 24 h. These results suggest that the VSMCs in vitro could adjust their behavious to adapt the tension.

In this study we examined the in vitro characteristics of tenocyte adhesion to biologically-modified surface of polymer. Polylactic-co-glycolic acid (PLGA) 85/15 films were prepared by a solvent-casting technique. Each film was adhered onto the bottom of a chamber. The film was precoated with poly-D-lysine (PDL), and then coated with serum-free F12 medium containing various concentrations of fibronectin (FN), type I collagen (CN I), and insulin-like growth factor1 (IGF-1). The monoclonal antibodies (to FN and to CN I) with various dilutions were used to inhibit attachment of tenocytes to surface precoated with FN or CN I. Human embryonic tendon cells (HETCs) and transformed human embryonic tendon cells (THETCs) were used as the seeding cells. The system used for the measurement of adhesion force was the micropipette aspiration experiment system. The micropipette was manipulated to aspirate a small portion of the tenocyte body by using a small aspiration pressure. Then the pipette was pulled away from the adhesion area by micromanipulation. The minimum force required to detach the tenocyte from the substrate was defined as the adhesion force. The results showed that modification of FN or CN I by precoating significantly enhanced attachment of tenocytes to surface of polymer (P < 0.05). As antibodies to FN or CN I were added to a polymer film precoated with FN or CN I, the adhesion force decreased significantly (P < 0.05). We concluded that the specific adhesion forces of tenocytes to extracellular matrix adhesion proteins (FN and CN I) had coordinated action and showed good dependence on their precoating concentrations, and were inhibited by the antibodies to these adhesion proteins. Films precoated with IGF-1 strongly accelerated the adhesion of tenocytes to polymer. These results indicate that the specific adhesion of tenocytes to polymer can be promoted by coating extracellular matrix adhesive proteins and insulin-like growth factor1. It is of great importance to construct tissue-engineered tendon.
Biocompatible Materials ; chemistry ; Cell Adhesion ; drug effects ; physiology ; Cells, Cultured ; Extracellular Matrix Proteins ; pharmacology ; Growth Substances ; pharmacology ; Humans ; Lactic Acid ; chemistry ; Polyglycolic Acid ; chemistry ; Polylysine ; pharmacology ; Polymers ; chemistry ; Tendons ; cytology ; embryology ; physiology ; Tissue Engineering

It is the infent of this study to establish a simple method for cultivation of rat pulmonary microvascular endothelial cells(PMVECs) and investigate the viscoelasticity of PMVECs. First, we obtained rat's peripheral pulmonary tissue, which then was cut into small pieces and cultured with 3 ml DMEM containing 20% bovine calf serum, 90 U/ml heparin, 4 mmol L-glutamine, 100 U/ml penicillin and 100 micrograms/ml streptomycin. Next, moved away the pulmonary tissue pieces 60 h later, and started passage 2-4 days after continued culture. Last, digested and separated PMVECs and studied viscoelastic coefficients of PMVECs by using micropipette aspiration technique. The results revealed that the cultured PMVECs showed regular cobblestone morphology and conformed with endothelial cells morphological characterization by phase contrast microscopy. PMVECs elastic modulus K1 was 49.3 +/- 9.2 Pa, K2 was 73.2 +/- 24.8 Pa, and it's viscosity factor mu was 19.2 +/- 7.2 Pa. s. These data demonstrate that it is feasible to cultivate PMVECs with tissue pieces method, and PMVECs is of greater rigidity.
Animals ; Cells, Cultured ; Elasticity ; Endothelium, Vascular ; cytology ; physiology ; Lung ; blood supply ; Male ; Rats ; Rats, Wistar ; Viscosity

Objective:To explore the clinical significance of the level and dynamic changes of N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) in the diagnosis and prediction of long‐term prognosis for patients with chronic obstructive pulmonary disease (COPD) combined with pulmonary heart disease among Han Chinese in Yunnan district .Methods:A total of 234 patients with COPD who were treated in The Second People′s Hospital of Qujing in Yunnan province during Jan .2013 and Dec .2013 ,were selected .Among them ,there were 98 patients with COPD combined with pulmonary heart disease ,80 patients with acute exacerbation of COPD ,and 56 patients with stable COPD .The clinical data ,results of blood gas analysis and echocardiography were recorded on admission and the levels of NT‐proBNP were measured .Results:The distances of 6 minute walk test(6MWT) in acute exacerbation of COPD and COPD combined with pulmonary heart disease groups were significantly less than that in stable COPD group(P< 0 .01) .The level of NT‐proBNP in COPD combined with pulmonary heart disease group was significantly higher than those in stable COPD and acute exacerbation of COPD group(P< 0 .01) .Kaplan‐Meier survival analysis showed that there was no significant difference regarding the rate of readmission and death during 12 months between the NT‐proBNP<1 000 pg/mL group and the NT‐proBNP≥1 000 pg/mL group(P>0 .05) .The rate of readmission and death during 12 months in the group with NT‐proBNP level decreasing ratio after treatment ≥30% was significantly higher than that in the group with decreasing ratio < 30% (P< 0 .05) .Conclusions: The detection of NT‐proBNP is of great significance to the early diagnosis of COPD combined with pulmonary heart disease .The dynamic changes of NT‐proBNP levels before and after the treatment are superior to single detection of NT‐proBNP level in predicting readmission rate and mortality risk for patients with COPD combined with pulmonary heart disease .

The mechanical properties of tumor cells adhering to extracellular matrix (ECM) are closely related with their invasion and metastesis. In this study we investigated the adhesive mechanical properties between hepatocellular carcinoma cells(HCC) and the collagen I coated surfaces from the viewpoint of cell cycle by coupling cellular biology and cellular mechanics, using micropipette aspiration and cell synchronization technique. The results showed that the synchronous G1 and S phase HCC cells were achieved by use of thymine-2-desoryriboside, colchicines sequential blockage method and double thymine-2-desoryriboside blockage method, and that the synchronous rates of G1 and S phase HCC amounted to 74.09% and 90.39% respectively. Within the ranges of dosing and timing in this study, the adhesion of HCC cells to collagen I displayed dose dependent and time dependent patterns. S phase cells had small force of adhesion to collagen I as compared with G1 phase and controlled cells(P<0.001), which suggested that G1 phase HCC may play an important role in the step of invading interstitial connective tissue in the metastasis pathway of HCC through blood circulation. These are of significance to unveiling the mechanism of HCC metastasis.
Animals ; Cell Adhesion ; Cell Cycle ; Collagen Type I ; metabolism ; Liver Neoplasms, Experimental ; metabolism ; pathology ; Neoplasm Metastasis ; Rats ; Tumor Cells, Cultured