Objective To compare the results of three different types of fine particulate matter laser light scattering aerosol monitors and to explore the influencing factors.Methods Three types of Aerosol Monitor A, B, and C were used to monitor the mass concentration of indoor PM2.5 at the same time and place and the membrane weighing method was used at the same time.In order to explore the possible impacts of temperature and humidity indoor on the results of monitoring, a simultaneous monitoring on the temperature and humidity indoor was conducted.The sampling continued 2 days and with 23 hours per day.Results The results of PM2.5 monitored by the three types of aerosol monitors had a good consistency(r were 0.965, 0.988, and 0.984 respectively), but there was significant difference among them(P

Objective:To study short-term effects of PM10(particulate matter of aerodynamic diameter

Objective To assess the effect of anticoagulant drugs,elastic compression stockings(ECS)and(Daflon) on the prevetion of the post-thrombotic syndrome(PTS).Methods Fifty-eight deep venous(thrombosis)(DVT) patients were divided into control group and treatment group I and II.The control group (n=15) did not take anticoagulant drugs or the time of anticoagulant drug administration was less than 1 month,and the use of(ECS) was less than 3 months.The treatment group I(n=24) took warfarin for 6 months and the ECS were used in the follow-up time;the treatment group II(n=19),besides warfarin therapy and ECS,took Daflon for 12 months.All the patients were followed up,the general conditions were assessed with clinical score,and the therapeatic results of the 3 groups were assessed.Results The rate of PTS occurrence in control group was significantly higher than that in treatment group 1 and treatment group 2.At 6 months,the clinical score of treatment groups 1 and 2 was significantly lower than that of control group.At 1 and 1.5 years after discharge,the clinical score of treatment group 2 was significantly lower than that of treatment group 1.Conclusions The long-term comprehensive and systemic therapy(including warfarin,ECS and daflon) for DVT could prevent PTS.

Traumatic hemothorax is a common disease in chest trauma .If it is not treated properly and effectively , it will lead to continuous blood loss, clotted hemothorax, empyema and other life-threatening conditions.Because of no universally ac-cepted guide or consensus in the treatment of traumatic hemothorax , clinicians often choose different ways to treat different de-grees and types of traumatic hemothorax according to their habits and experience .This article focus on recent studies on the treatment of traumatic hemothorax , summarize the latest achievements in the field of traumatic hemothorax , provide evidence for clinicians'clinical decision-making, and find the current insufficiency of traumatic hemothorax and the direction for further re-search.

In order to increase the expression level of target gene and to simplify the purifying process of separation and purification, we performed the transgenetic research of antigen VP7 gene into peanut via Agrobacterium tumefaciens. The plant binary expression vector is pBOG3VP7 harboring fusion gene oleosin-vp7, which is promoted by ole-promoter. Cotyledon nodes were used as transformation recipients. Transformed individuals were obtained through selection on medium containing 125 mg L-1 Kan. Integration of transgenes was assessed by PCR amplification and PCR-Southern blot hybridization. Taking pBOG3VP7 plasmid as positive control, non-transformed peanut as negative control. 6 plants among 11 plants grown up through seletion medium were detected by PCR and the rate of positive plants is 54.5%. PCR positive plants were further analysed by PCR-Southern blot hybridization. The results showed that 3 plants have DNA bloting bands. The results also showed that the foreign gene was integrated into genome of transformed peanuts. Elevated expression of rotavirus VP7 antigen in transgenic peanuts was a critical factor in the development of efficient and cheap plant oral vaccine.
Agrobacterium tumefaciens ; genetics ; Antigens, Viral ; biosynthesis ; genetics ; Arachis ; genetics ; metabolism ; Capsid Proteins ; biosynthesis ; genetics ; Plants, Genetically Modified ; genetics ; metabolism ; Rotavirus ; genetics ; immunology ; Transformation, Genetic ; Vaccines, Synthetic

Antibodies, Monoclonal ; chemistry ; immunology ; Antigens, Viral ; chemistry ; genetics ; immunology ; Binding Sites ; Capsid Proteins ; chemistry ; genetics ; immunology ; Epitopes ; chemistry ; genetics ; immunology ; Escherichia coli ; genetics ; metabolism ; Gene Expression ; Hepatitis E ; immunology ; prevention & control ; virology ; Hepatitis E virus ; chemistry ; immunology ; Humans ; Molecular Docking Simulation ; Mutagenesis, Site-Directed ; Peptide Mapping ; Protein Binding ; Recombinant Proteins ; chemistry ; genetics ; immunology ; Viral Hepatitis Vaccines ; administration & dosage ; biosynthesis

Objective　This study retrospectively analyzed reported cases of subacute combined degeneration of spinal cords(SCD).The purpose of this study was to provide theoretical basis for the prevention,diagnosis,treatment and research of SCD.Methods　We searched the PubMed database for terms related to subacute combined degeneration of spinal cords from March 2,2019 to January 1,1990.255 cases were finally included in the study.Results　The common causes of SCD were Vitamin B12 deficiency (128 cases),inhaled N-2O (70 cases) and copper deficiency (31 cases).The disease usually happened on middle-ager (44.5±19.9) years old.The course of the disease is relatively long (36.5±85.1) weeks.Limbs hypoesthesia was the most common symptom (73.3%),followed by ataxia (61.6%),and limb motor system could also be involved (35.3%).Autonomic nervous system,cranial nerve,consciousness disorder and cognitive disorder are rare.The patients were often complicated with giant cell anemia (72.5%) and hyperhomocysteinemia (80.9%).Spinal cord MR could show abnormal signal shadow in posterior cord (82.9%),most of which occurred in cervical cord.Conclusion　Vitamin B12 deficiency was the most common cause of SCD in adults and N-2O abuse must be paid attention in young people.Most patients of SCD showed peripheral nervous system damage that sensory system damage is common.Abnormal signal shadow in posterior cord of spinal MR was specific for the diagnosis of SCD.Most patients with SCD have a good prognosis after treatment.

Objective To investigate associations of herpes simplex virus(HSV)infection with elevated high-sensitivity C-reactive protein(hsCRP)level and the development of atrial fibrillation(AF) in elderly individuals.Methods Through screening a total of 2,603 individuals,49 patients aged 60 years or over with AF and without structural heart diseases and known risk factors for AF were selected for data collection.Fifty-five elderly healthy control subjects were selected from a population based Chinese Arrhythmia Registry.Baseline characteristics,plasma antibody levels against HSV-1 and-2,and level of hsCRP were analyzed.Results Level of antibodies against HSV-1 was significantly higher in AF patients[(314.5±63.6)mg/L than in control subjects(216.0±50.7) mg/L,P＜0.01].Level of antibodies against HSV-2 was significantly higher in AF patients[(400.1±50.7) mg/L than in control subjects (306.3 ± 64.5) mg/L,P ＜ 0.01].Linear regression analysis demonstrated a positive correlation of plasma levels of hsCRP with both anti-HSV-1 and anti-HSV-2 IgG antibodies (r =0.291,0.188,all P ＜ 0.01).In multiple logistic regression analysis,HSV-1 and HSV-2 antibody levels were significantly associated with AF occurrence(OR =1.035 and 1.034,all P ＜0.01).The optimal cut-off point for predicting AF occurrence was 284.96 mg/L for anti-HSV-1 with sensitivity of 67.3％ and a specificity of 92.7％,and was 366.26 mg/L for anti-HSV-2 with a sensitivity of 67.3％ and a specificity of 85.5％,respectively.Conclusions The increased plasma levels of anti-HSV-1 and anti-HSV-2 antibodies are significantly associated with AF occurrence in elderly adults.

Objective:To investigate the risk factors associated with the complications of video-assisted thoracic surgery (VATS) in the treatment of traumatic hemothorax.Methods:A retrospective case-control study was conducted to analyze the clinical data of 94 patients with traumatic hemothorax undergone VATS treatment in Tangdu Hospital of Air Force Medical University from January 1, 2012 to December 31, 2018. There were 75 males and 19 females, aged from 15-78 years [(45.8±14.6)years]. Postoperative complications occurred in 12 patients (complication group) and not in 82 patients (non-complication group). The following data were analyzed for complication-related risk factors using univariate analysis and Logistic regression analysis, including gender, age, mechanism of injury, pneumothorax and hemothorax, number of fractured ribs, abbreviated injury score (AIS), injury severity score (ISS), time from injury to operation, operation time, rib fixation or not, pulmonary air leakage or not, duration of drainage, postoperative volume of drainage, and postoperative length of hospital stay.Results:The proportion of patients with time from injury to operation (>3 days), duration of drainage and postoperative length of hospital stay in complication group [75%(9/12), (7.8±2.6) days, (10.2±2.7)days] were significantly higher or longer than those in non-complication group [37%(30/82), (5.5±1.6)days, (8.0±2.3)days] ( P<0.05 or 0.01). There were no statistically significant differences between the two groups in gender, age, mechanism of injury, pneumothorax and hemothorax conditions, number of fractured ribs (≥3), AIS, ISS, operation time, rib fixation or not, pulmonary air leakage or not and postoperative volume of drainage ( P>0.05). Logistic regression analysis showed time from injury to operation (>3 days) and pulmonary air leakage were significantly associated with the complications of VATS ( P<0.05). Conclusion:Time from injury to operation (>3 days) and pulmonary air leakage are independent risk factors for the complications of VATS in the treatment of traumatic hemothorax.

Objective:The characteristics of women with false elevated testosterone were analyze and the literature was reviewed to provide reference for clinical laboratory identification of false elevated testosterone.Methods:The characteristics of three patients with false elevated testosterone in Peking Union Medical College Hospital were analyzed retrospectively, and the results of different detection platforms and methods for the determination of testosterone levels were compared. International and domestic literatures related to false elevation of testosterone and detection methods of testosterone were searched for a comprehensive analysis from PUBMED and CNKI.Results:The levels of testosterone in 3 female patients were elevated by immunoassay and normal by mass spectrometry. They were excluded from the diagnosis of hyperandrogenemia. A total of 38 literatures related to testosterone detection were retrieved, of which 9 case reports of pseudohyperandrogenemia, among which 12 cases of pseudohyperandrogenemia were reported in 2 domestic literatures in 2021. All cases were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Previous studies have clearly indicated that the result of routine immunoassay in clinical laboratory for the determination of female testosterone have poor correlation with the results of LC-MS/MS, with varying degrees of deviation.Conclusions:Immunoassay tests for female testosterone is susceptible to interference and lead to elevated false results. It is suggested that clinical laboratories evaluate the detection methods used and establish a identification program, and confirm samples with suspected pseudoelevated testosterone elevation using other immune platforms or LC-MS/MS.