Objective To investigate the prevalence of trematode Aspidogastrea in freshwater mussels in Anhui Province, China. Methods The freshwater mussels living in different water areas within Anhui territory were harvested and dissected from April to May,2015. Then the flukes were collected from the pericardial cavities of the clams into a petri dish containing small amount of saline. After rinsing,the flukes were transferred into a bottle containing 70%ethanol for following identification by staining of the specimens prepared as previous protocol. Results A total of 3 007 calms in 12 species,including Unio doug?lasiae(603),Acuticosta chinensis(90),Lamprotula caveat(150),Lamprotula leai(250),Lanceolaria grayana(47),An?odonta pacifica(60),Anodonta woodiana(350),Cuneopsis heudei(100),Solenaia oleivora(150),Cristaria plicata (567),Hyriopsis cumingii(550)and Arconaia lanceolata(90)were dissected,in which 1 467 mussels were infected with the trematode Aspidogastrea,with an infection rate of 48.79%(1 467/3 007). Totally,7 306 flukes belonging to 3 genera under the subclass of Aspidogastrea were isolated. These trematodes were genera of Aspidogaster,Lophotaspis and Cotylaspis. The in?fection density was from 1 to 128 capita for individual positive clam,with a mean infectiosity of 4.98(7306/1467)for each mus?sel. Conclusion The freshwater bivalves living in different water areas in Anhui Province are infected with the trematode Aspi?dogastrea. These flukes were identified as Aspidogaster sp. Lophotaspis sp. and Cotylaspis sp. belonging to subclass Aspidogas?tridae of family Aspidogastrea.

We reported a case of adult Drosophila melanogaster parasitized in nasal cavity of a 81-year-old woman who was living in Xuancheng City, Anhui Province now. She was admitted for treatment of cerebral infarction and water accumulation in the lungs in 2014 June. The patient was also suffering from secretory otitis media, a history of hypertension and heart stents were placed in 2007. A foreign body was found in the left nasal cavity during the preoperative examination process, and then the part of the inflammatory tissue was removed through the nasal endoscopy, and sent to our department for identification. There are three adults of Drosophila in paraffin-embedded biopsy specimens. The parasites length is approximately 3mm, with huge red compound eyes. The end of the body is tip, with 5 ring lines in back, has no dark spots. The abdomen of the parasites have seven sections. Tarsus of foot I have no sex comb on base, and they are male adult of Drosophila melanogaster after identification. After a thorough reviewing of medical history, we knew the patient began to sneeze violently and frequently six years ago. But there was no clear or purulent nasal discharge flowing, therefore did not attract attention. After removing the parasites the sneezing symptoms were relieved, and had no abnormal symptoms in the follow-up 6 months.
Aged, 80 and over ; Animals ; Drosophila melanogaster ; growth & development ; Endoscopy ; Female ; Foreign Bodies ; diagnosis ; parasitology ; Humans ; Male ; Nasal Cavity ; parasitology

Objective To synthesize a new-type thermosensitive liquid embolic material and to investigate the feasibility of using this material to occlude cerebral arteriovenous malformations (AVMs).Methods The copolymer was synthesized with N-isopropylacrylamide (NIPAM) and N-n-propylacrylamide (NNPAM),and it's physical and biological properties were estimated.The embolization of AVMs model in vitro by using this copolymer was conducted and the results were analyzed.Results The new-type copolymer possessed unique thermal behavior of lower critical soluble temperature (LCST),and it was water-soluble and non-adhesive with better biocompatibility.The successful embolization of AVMs model could be reliably obtained.Conclusion The copolymer synthesized by the authors is a new-type liquid embolic agent suitable for endovascular embolization of cerebral AVMs in vitro.Based on the results in experiment animals having been reported in medical literature,this copolymer can be further used in clinical research.

Objective To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City so as to provide the evidence for its prevention and control. Methods From March to June and September to De?cember in 2014 dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated identified and counted. Results Totally 360 samples were selected and 169 samples 46.94% had mite infestation. Pyroglyphidae accounted for the most in the population with the breeding rate of 45.00%. Totally 18 504 mites were found and Dermatophagoides farina and Dermatophagoides pteronyssinus were the dominant species with the composition ratios of 30.21%5 590 mites and 17.83% 3 300 mites respectively. The third?ranked kindergartens were in the most serious situation con?cerning with 81.67% 98/120 of the breeding rate of Acaroid mites. The differences were statistically significant among the kin?dergartens with different ranks F=6.048 χ2=73.523 both P<0.05 . There were no significant differences about the Aca?roid mite population among different grades in kindergartens F=0.132 χ2 =2.377 both P>0.05 . Conclusion Acaroid mites in the kindergartens in Wuhu City were in serious condition and the population and species composition of Acaroid mite community is influenced by the human behavior and indoors environments.

Objective To investigate the epidemic situation of Metorchis orientalis in Wuhu City Anhui Province. Meth?ods Three lakes Fengming Longwo and Kui lakes were selected in Wuhu City and the poultries around the lakes and fresh?water fishes in the lakes were captured to test the infection of M. orientalis and the infection rates were calculated. Results The adult worms of M. orientalis were found in gall bladder and hepatic duct of domestic ducks with infection rate of 16.7%. The metacercariae of M. orientalis were discovered in the muscle of Pseudorasbora parva and Pseudogobio rivularis with infection rates of 7.13%and 3.38%respectively. Conclusion M. orientalis is endemic in Wuhu City which should be paid enough at?tention to the animal husbandry aquaculture and medicine.

Objective To study the suitable dose of intracoronary adenosine (AD) on fractional flow reserve (FFR) measurement in Chinese patients with angiographic coronary artery disease. Methods FFR was measured in 32 patients with 40 moderate coronary stenosis. Boluses of intracoronary AD at increasing doses of 60μg (A1), 80μg (A2), 100μg (A3) and 120μg (A4) were randomly administered. FFR values, symptoms, systemic effects and development of atrioventricular block were recorded. Results FFR value decreased significantly by 8.99%(A1), 11.24%(A2), 13.48%(A3) and 13.48%(A4) compared with the baseline distal coronary pressure/aortic pressure (0.891±0.044, all P<0.001). A3 and A4 showed significantly lower FFR values than A1 (t=6.331、6.343, all P < 0.001),A2 (t=2.974、3.058, P=0.005、0.004). Positive rates of an FFR of<0.75 were 30.0%(n=12), 32.5%(n=13), 35.0%(n=14) and 35.0%(n=14) in A1, A2, A3 and A4. A total of 13 patients(40.6%) reported at least one side effects. Conclusions This study suggests a dose-response relationship for intracoronary AD on the measurement of FFR in Chinese patients with angiographic coronary artery disease. The suitable dose of bolus of intracoronary AD is 100μg.

Objective To investigate the species of Acaroid mites breeding in the stored traditional Chinese animal medici?nal materials and the relationship between its community and habitats. Methods A total of 30 samples of traditional Chinese animal medicinal herbs were collected from Wuhu City,Anhui Province,China. The mites were isolated by the directly micro?scopic and floatation microscopic examinations,and then identified and counted under a light microscope. Results Acaroid mites was represented in 28 of the 30 samples,and the breeding rate accounted for as high as 93.3%(28/30). Totally,13 spe?cies of Acaroid mites were identified,which belonged to 4 families and 9 genera. The densities of Acaroid mites were top in 6 Chinese herbal medicines,such as corium erinacei,aspongopus,hirudo,pheretima aspergillum,Apostichopus and huechys. The diversity parameters of these six traditional Chinese animal medicinal herbs were calculated. The highest richness indexes were in aspongopus and hirudo,the highest diversity index was in hirudo,and the highest evenness index was in Apostichopus. Conclusions There are Acaroid mites breeding in parts of the traditional Chinese animal medicinal herbs stored in Wuhu. In the storage and processing of Chinese herbal medicines,we should pay attention to the prevention and control of mites.

Objective To characterize the population dynamics and spatial distribution of Aleuroglyphus ovatus in the flour warehouse,so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitor?ing and management. Methods The samples from flour warehouses of four localities were collected,examined and counted for A. ovatus in every month in Wuhu City. The dispersion pattern target,Iwao m*/-x regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of A. ovatus in the flour warehouses. Results The peaks of population dynamics of A. ovatus were discovered in July and September,respectively. The indexes of dispersion were as follows:I>0, CA>0,m*/-x >1. At the same time,the parameters in the equation of Iwao:m*=5.471+1.022-x (r=0.999)and Taylor:lgS2=0.697+1.111 lg-x (r=0.987)showed that the spatial distribution pattern of A. ovatus was assembled. Conclusion The peaks of population dynamics of A. ovatus in the flour warehouse are bimodal pattern ,and the spatial distribution pattern of A. ovatus is assembled.

Objective To investigate the species of Cheyletoidea mites breeding in the culture environment of Eupolyphaga sinensis and to observe the morphology of Eucheyletia reticulata Cunliffe. Methods The soil samples from an E. sinensis farm in northern Anhui were collected. The mites in the soil samples were separated directly under a microscope and the glass speci?mens were made to observe the morphological feature of the mites under a light microscope,then the mites species were identi?fied and classified based on the morphological characteristics. Results In the culture soil of E. sinensis,totally 7 kinds of Chey?letoidea mites were isolated,namely Eucheyletia reticulata Cunliffe,Cheyletus eruditus Schrank,Cheyletus malaccensis Oudemans,Cheyletus troussarti Oudemans,Cheyletus aveisor Rohdendorz,Acaropsis sollers Rohdendorz and Cheletomor?pha lepidopterorum Shaw. They belonged to genera Eucheyletia,Cheletomorpha,Acaropsis and Cheyletus of Cheyletidae Leach family. The Eucheyletia reticulata Cunliffe was firstly found in the culture environment of E. sinensis,and its gnathosoma was large,the pedipalpal femurs were expanding and there were two strips of comb hair and two smooth bristles on the pedipal?pal tarsus,and the back of the body was covered with two pieces of tergum,which were decorated with reticular pattern. The body and foot setae were fan?shape. Conclusions There are various of Cheyletoidea mites found in the breeding environment of E. sinensis. These mites are important species for pest control in the culture environments of E. sinensis. Related measures should be taken to prevent the excessive growth of Cheyletoidea mites,so as to avoid the adverse effects on the quality and quan?tity of E. sinensis.

Objective To investigate the Suidasia nesbitti breeding status in the ground dust flour collected in Qiqihaer City. Methods Totally,16 aliquots of ground dust flour were respectively collected from a college canteen and 15 households in Qiq-ihaer City. Then 10 g dust flour was taken from individual sample for isolation of the mites that were made of slide specimen,and the mites were identified and classified under a microscope. Results Acaroid mites were found in 15 of the 16 aliquots of sam-ples(detection rate being 93.8%),and 7 species,belonging to 6 genera under 3 families,were identified. A total of 561 heads of mites were isolated from 160 g samples,with an average breeding density of 3.51 heads/g. The most breeding mite was associ-ated with S. nesbitti. Conclusions Various species of mites are breeding in the ground dust flour in Qiqihaer City,and S. nesbit-ti occurs the most. These findings indicate that effective measures should be taken to prevent and control the harm associated with acaroid mite contamination.