Objective To observe the hypoglycemic, hypolipidemic and antioxidant effects of Zhuye Shigao Decoction (ZSD) on experimental type 2 diabetes mellitus rats. Methods Type 2 diabetes mellitus rat model was induced by intragastric administration of high fat diet(10 mL/kg) and intraperitoneal administration of streptozotocin (30 mg·kg-1). Sixty male Sprague-Dawley rats were randomized into normal group, model group, low-, moderate-and high-dose ZSD groups (in the dosage of 3.6, 7.2, 14.4 g·kg-1·d-1 respectively), and Metformin group (0.15 g·kg-1·d-1) , 10 rats in each group. With fasting blood glucose, urine glucose, glycosylated hemoglobin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol(LDL-C), serum insulin, serum superoxide dismutase(SOD) and malondialdehyde (MDA) as the main indexes, we evaluated the influence of ZSD on the blood glucose and lipid levels as well as oxidative stress in type 2 diabetes mellitus rats. Results Compared with the model group, the levels of blood glucose, urine glucose and glycosylated hemoglobin, and the contents of TC, TG, MDA were decreased (P <0.05), whereas SOD activity and serum insulin level were obviously increased (P < 0.05) in ZSD groups. ZSD showed dose-related effectiveness in the treatment of diabetic rats. Conclusion ZSD has certain hypoglycemic, hypolipidemic and antioxidant effects in treating type 2 diabetes mellitus.

Objective To evaluation the precision performance of XE-2100 Automatic Blood Cell Analyzer using EXCEL Mi-crosoft with reference to CLSI EP15-A3 .Methods According to CLSI EP15-A3 ,high level and low level (BIO-RAD company) of quality control materials were measured using XE-2100 Automatic Blood Cell Analyzer ,and analyzed by one-factor analysis of vari-ance of EXCEL 2003 and equation editor .The within-run coefficient of variation and within-laboratory coefficient of variation were calculated and compared with national industry standards and manufacturer statement .Results For high and low level of quality control materials ,the within-run coefficient of variation and within-laboratory coefficient of variation of white blood cell ,red blood cell ,hemoglobin and platelet were less than national industry standards and manufacturer statement .Conclusion The precision ver-ification scheme of CLSI EP15-A3 with EXCEL Microsoft has a relatively good maneuverability and statistical performance ,which could be used in verification of the precision performance of quantitative testing items in clinical laboratories .

OBJECTIVE: To develop a fiber Raman endoscopic probe that can be integrated in a gastroscope and evaluate its value in the diagnosis of gastric cancer. METHODS: The Raman spectra of gastric cancer tissues and normal tissues were obtained using the fiber Raman endoscopic probe and confocal microRaman spectroscopy. After preprocessing with smoothing, baseline elimination and normalization, the spectroscopic data were analyzed by the principle component analyses combined with stechiometry. Based on the pathological results, the diagnostic accuracy, sensitiveness and specificity of Raman spectroscopy combined with stechiometry were evaluated. RESULTS: The fiber Raman endoscopic probe and microRaman spectroscopy revealed significantly different Raman spectra between gastric cancer tissues and normal tissues. The diagnostic accuracy, sensitiveness and specificity of the fiber Raman endoscopic probe was 80.56%, 88.89%, and 84.72% for gastric cancer, respectively. CONCLUSIONS The fiber Raman endoscopic probe combined with stechiometry provides an effective modality for the diagnosis of gastric cancer and can well distinguish gastric cancer tissue from normal gastric tissues.
Endoscopy ; Fiber Optic Technology ; Humans ; Sensitivity and Specificity ; Spectrum Analysis, Raman ; Stomach Neoplasms