Objective To investigate the effect of nerve growth factor ( NGF) combined with exercise train-ing on nerve protection, learning and memory. Methods Twenty-four six-month-old APP/PS1 mice were randomly divided into a control group, an NGF group, an exercise group and an NGF combined with exercise training group ( the combined group) , each of 6. The control group mice were given 50 μL of saline intra-nasally every day, while the NGF group were given 50 μL of NGF solution (0.1 mg/ml) intranasally. The exercise group was given 50μL of saline intra-nasally plus 30 minutes of treadmill exercise daily, while the combined group was given 50μL of the NGF solution and 30 minutes of exercise. The intervention lasted 21 days. Then Morris water maze test was conducted to e-valuate the memory of all groups, and all of the mice were sacrificed and immunohistochemical staining was used to check the levels of APP, Ki-67 expression in cortex and NeuN expression in hippocampus. Results In the place navigation test, significant differences in escape latency were observed among the 4 groups, with the NGF, exercise and combined groups showing average latencies significantly shorter than that of the control group The average latency of the combined group was also significantly shorter than that of the NGF and exercise groups. In the spatial probe, there were significant differences among the 4 groups in the percentage of time spent in the target quadrant. The com-bined group′s average was significantly larger than those of the NGF and exercises groups. The immunochemistry showed that the combined group had better-arranged neurons and more cells compared with the 3 other groups. The average APP protein expression of the combined group was significantly lower than that of the other groups, but their Ki-67-positive and NeuN-positive cells were found to be significantly more numerous. Conclusion NGF combined with exercise can improve learning and memory, at least in APP/ PS1 mice. This might be associated with its reduc-ing the expression of APP and promoting the expression of Ki-67 and NeuN.

Objective To evaluate the influence of various shades of resin cements on the final color of an improved lithium-disilicate pressed glass ceramic veneers and analyze the agreement of resin cements and corresponding try-in pastes.Methods Forty-eight artificial maxillary central incisor teeth were sequenced according to the measured color parameters and divided at random into 8 groups(n=6).These artificial teeth were prepared following veneer preparation protocol.An improved lithium-disilicate pressed glass ceramic materials(IPS e.max Press,Ivoclar Vivadent) were selected as the veneer material.The shape and curvature of each veneer wax pattern were duplicated with the same impression to guarantee the similarity.The ceramic veneer specimens were delivered on the artificial teeth using the corresponding try-in pastes of 8 shades(Variolink Veneer,shades of LV-3,LV-2,MV,HV+2,HV+3; and 3M RelyXTM Veneer,shades of WO,TR,A3) and bonded with the resin cements.A clinical spectrophotometer was used to measure the color parameters of the ceramic veneers before the try-in,during the try-in procedure,and after cementation.△E values and C*ab values were calculated.Results The result of one-way ANOVA indicated that the color changes of ceramic veneer cementation with resin cements were statistically significantly different in the shades of resin cements(P＜0.001).The △E values of ceramic veneer after cementation ranged from 0.93 to 6.79.The color changes of ceramic veneer specimens using the shades of LV-3,HV+3,WO were 3.31,4.90 and 6.79,respectively(△E＞3.3).The △E values of the ceramic veneer specimens between the resin cements and corresponding try-in pastes were from 0.72 to 1.79(except the shade of HV + 3).Conclusions The LV-3,HV+3,WO shades were able to change the final color of a ceramic veneer.The color of resin cements and corresponding try-in pastes achieved high agreement(except the shade of HV+3).

Objcetive: To explore the treatment of long segment defect of tibia by using tensor fascia lata combined with iliac flap or deep circumflex iliac pedicle iliac flap. Methods: From February 2012 to August 2017, The People′s Hospital of Zun Yi City Bo Zhou District treated 16 patients who had long segment defect of tibia.There were 11 males and 5 females, age from 22 to 58 years old, the average age was 42 years old. Iliac flap grafting with tensor fascia lata combined with iliac flap or deep circumflex iliac pedicle was used to treat the defect of long segment of tibia. There were 4 cases with simple tibial defect and 12 cases with skin defect. The longest tibial defect was 5-8 cm. Results: In this study, four patients used iliac flaps with deep circumflex iliac pedicle, the area of flaps ranged from 2.5 cm×5.0 cm to 5.0 cm×10.0 cm, while the area of iliac flaps ranged from 5.0 cm×2.5 cm to 8.0 cm×4.0 cm. Twelve patients used grafting with tensor fascia lata combined with iliac flap, the area of flaps ranged from 5.0 cm×12.0 cm to 12.0 cm×23.0 cm, while the area of iliac flaps ranged from 7.0 cm×2.0 cm to 8.0 cm×4.0 cm. All 16 cases of bone flap were survived, fracture healing, without surgical complications. The average follow-up period was 1.5 years, the flaps had good appearance in 10 cases and was slightly bloated in 6 cases; the ankle had normal motion in 14 cases and had poor dorsal extension in 2 cases. X-ray films showed that the bone flap repaired the bone defects and reached bone healing. Conclusions Vascularized tensor fascia lata combined with iliac flap or deep circumflex iliac pedicle iliac flap grafts increase local blood supply and accelerate the process of fracture healing.