2.A Frequency Weighting Contour for Chinese Young Males
Shaoping PENG ; Yingzhong LI ; Yibao HAN ; Yanhua XIAO
Space Medicine & Medical Engineering 2000;13(3):162-165
Objective To obtain the frequency weighting contour for seated Chinese young males in z axis.Method 10 male volunteers served as the subjects.They were exposed to standard vibration stimulus (a sinusoidal vibration of 1.0 m/s2 at 8 Hz) and 14 kinds of sinusoidal experimental vibration stimuli at frequencies from 4 Hz to 80 Hz.The magnitude of the experimental vibration stimulus was changed by the subject through a signal condition and switching device made by ourselves to obtain an equal vibration sensation to the standard vibration.The data were fitted to the same form of the frequency weighting Wk of ISO 2631-1 with the least square method and optimization of parameters.Result Compared with ISO 2631-1,the frequencies weightings of Chinese young males at 16~80 Hz are significantly higher than those of ISO 2631-1(P<0.005),but there are no significant difference between two frequency weighting contours at other frequencies. Conclusion Chinese young males are more sensitive to vibration at 16~80 Hz in Z axis.
3.Effect of different concentrations of neogenin on proliferation, apoptosis and related proliferative factors in human trophoblasts.
Shaoping, ZHONG ; Li, ZOU ; Yin, ZHAO ; Bin, HU ; Han, XIE
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(4):500-4
The underlying effect of different concentrations of neogenin on proliferation, apoptosis and the related proliferative factors in human trophoblasts was explored in order to understand the function of neogenin during placentation. TEV-1 cell line was cultured and the expression of netrin-1 was detected by using indirect cellular immunofluorescence. Exponentially growing TEV-1 cells were treated by different concentrations of neogenin (0, 1, 5, 10, 50 ng/mL) for 24 h. Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. TEV-1 cell apoptosis was assessed by flow cytometry (FCM). The expression of netrin-1 mRNA and protein in TEV-1 cells was examined by using real-time PCR and Western blot, respectively. It was found that immunoreactivity for netrin-1 was observed in cytoplasm of the trophoblasts. Immediately after treatment with different concentrations of neogenin for 24 h, the netrin-1 expression began to increase. Real-time PCR revealed that the expression level of netrin-1 mRNA was 37.59+/-10.25 times higher than control group when TEV-1 cells were exposed to 50 ng/mL neogenin (P<0.01), and the same tendency was seen by using Western blot. MTT results showed that proliferation of TEV-1 cells was independent of neogenin. Meanwhile, apoptosis was significantly increased to (22.15+/-6.15)% at 50 ng/mL neogenin and (6.55+/-0.25)% without neogenin (P<0.01). It is suggested that neogenin regulates proliferation and apoptosis of TEV-1 cells. And it can enhance the ability of TEV-1 cells to express netrin-1 in a dose-dependent manner. Neogenin may play an important biological role in the normal human pregnancy and contribute to the physiological pregnancy process.
4.Reform and exploration on forensic toxicology teaching
Shaoping HAN ; Jingjun XING ; Shaohua ZHU ; Xinshan CHEN ; Liang REN ; Yiwu ZHOU
Chinese Journal of Medical Education Research 2012;11(9):934-936
As a core curriculum of forensic medicine,it is of great importance to improve the quality of forensic toxicological education for cultivating high-quality forensic professionals.Basing on the rich experiences of forensic teaching and expertise,well teaching effects were achieved by the reform and explorations in teaching contents and methods,development of curriculum and teachers as well as other aspects aimed to improving the quality of forensic toxicological teaching.
5.In vivo and in vitro research study of the influence on placenta angiogenesis by gene silencing of netrin-1
Han XIE ; Li ZOU ; Jianwen ZHU ; Yun YANG ; Shaoping ZHONG ; Qianhua WANG
Chinese Journal of Obstetrics and Gynecology 2011;46(5):364-369
Objective To study influence on angiogenesis of placenta by gene silencing of netrin-1.Methods Netrin-1 gene in human umbilical vein endothelial cells(HUVEC)and placenta of pregnant rats were silenced by RNA interference.The following methods were used in this study,including the phenytetrazoliumromide(MTT)for viability,clone formation for proliferation,transwell for migration,and tube formation for angiogenesis in vitro.The change of fetal growth was recorded.Placental microvessel density in pregnant rats was measured by immunohistochemical CD34 staining in vivo.Results (1)HUVEC:viability and proliferation of HUVEC were remarkably inhibited by gene silencing of netrin-1.which number of clone formation,migration cell,tube formation were from(69±6)%,86±17,37±9 decreased to(46±5)%,46±13 and 17±5(P<0.05)respectively.(2)Placenta of pregnant rats:after netrin-1gene silenced,fetal weight were decreased from(2.39 ±0.17)g to(2.12±0.10)g(P<0.05).Placental microvessel density was decreased from(258±38)/mm2 to(197±32)/mm2 in vivo(P<0.05).Conclusions Gene silencing of netrin-1 could inhibit viability,proliferation,migration,tubal formation of HUVEC and angiogcnesis of placenta.Netrin-1 plays an important role in regulating angiogenesis in placenta.
6.A lipophilic fraction of Ganoderma lucidum promotes PC12 differentiation
Li ZHANG ; Shaoping PENG ; Rong HAN ; Jianhua QIU ; Zhenlun GU ; Zhenghong QIN
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To investigate the effects of lipophilic fraction of Ganoderma lucidum on PC12 differentiation. Methods Rat PC12 cells were cultured in DMEM medium. Differentiation of PC12 cells was evaluated by monitoring neurite outgrouth of PC12 cells with phase-contrast microscopy after treatment with lipophilic extracts of Ganoderma lucidum or it's effective component B_2. Expression of neuronal marker,microtubule-associated protein-2,was determined with immonofluorescence and western blot analysis. Results Lipophilic extracts of Ganoderma at the concentrations between 125 and 500 mg?L -1 induced neurite outgrowth of PC12 cells in a dose-dependent manner. Nearly all cells showed morphological differentiation in response to lipophilic extracts of Ganoderma. Lipophilic extracts of Ganoderma also potentiated NGF-induced differenti-ation of PC12 cells. Lipophilic extracts of Ganoderma increased the expression of MAP-2. Conclusion Lipophilic extracts of Ganoderma lucidum can induce differentiation of PC12 cells and potentiate NGFs actions.
7.Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China
Deng YONG ; Chen CUNWU ; Chen LINGXIAO ; Han BANGXING ; Li SHAOPING ; Zhao JING
Journal of Pharmaceutical Analysis 2021;11(3):284-291
Due to the extensive use of xylooligosaccharides (XOS) as functional food ingredients,many inferior goods and even adulterants are generally found in the market,which may pose a health hazard to certain populations.Chromatography method such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) is traditionally applied for the quality analysis of XOS.However,it is time consuming due to the prolonged separation and pre-or post-derivatization procedure.In this study,a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China.The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate,at least 6 times faster than that by the traditional HPTLC chromatography method.In addition,MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z,which is hardly separated using HPTLC.The results showed that XOS were present only in samples XY01-XY11,samples XY12-XY14 only consisted of hex oligo-saccharides,and samples XY15-XY22 were free of oligosaccharides.These indicate that the quality consistency of XOS products in the China market was poor,which should be carefully investigated.
8.Anti-tumor Angiogenesis with a Recombinant Ag43/FGFR1 Chimeric Protein As a Model Antigen
ZHENG SHAOPING ; WENG ZHIHONG ; ZHENG SHAOJIANG ; GUO JUNLI ; HUANG FENGYING ; XIE MINGXING
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(1):25-28
In order to investigate the anti-tumor angiogenesis activity with a recombinant Ag43/FGFR1 chimeric protein(AF)vaccine in a mouse H22 hepatoma model,tumor volume and survival rate of the mice were studied at a 3-day interval,Microvessel density(MVD)was detected by immunohistochemistry.The endothelial deposition of autoantibodies within tumor tissues was examined by immunofluorescent staining,and anti-FGFR1 antibody-producing B cells(APBCs)were tested by enzyme-linked immunospot(ELISPOT)assay.Compared with the three control groups,the tumor volume was significantly decreased and the survival time was significantly prolonged in AF-immunized group(P<0.05).The number of APBCs in AF-immunized mice(129.6±10.9)was more than in controls[6.2±1.1(FGFR1),6.0±1.2(Ag43)and 5.2±1.4(NS),P<0.01].Moreover,the endothelial deposition of autoantibodies was found in tumor tissues from AF-immunized mice,but not in control groups.MVD in AF-immunized group was significantly lower than in FGFR1-immunized group,Ag43-immunized group and NS group(10.3±3.1 vs 39.4±8.6 vs 42.3±9.8 and 43.6±10.6,P<0.01).These findings demonstrated that the AF protein vaccine effectively inhibited tumor angiogenesis and growth via production of autoantibodies against self-FGFR1.
9.Effect of Different Concentrations of Neogenin on Proliferation,Apoptosis and Related Proliferative Factors in Human Trophoblasts
ZHONG SHAOPING ; ZOU LI ; ZHAO YIN ; HU BIN ; XIE HAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(4):500-504
The underlying effect of different concentrations of neogenin on proliferation,apoptosis and the related proliferative factors in human trophoblasts was explored in order to understand the function of neogenin during placentation.TEV-1 cell line was cultured and the expression of netrin-1was detected by using indirect cellular immunofluorescence.Exponentially growing TEV-1 cells were treated by different concentrations of neogenin(0,1,5,10,50 ng/mL)for 24 h.Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay.TEV-1 cell apoptosis was assessed by flow cytometry(FCM).The expression of netrin-1 mRNA and protein in TEV-1 cells was examined by using real-time PCR and Western blot,respectively.It was found that immunoreactivity for netrin-1 was observed in cytoplasm of the trophoblasts.Immediately after treatment with different concentrations of neogenin for 24 h,the netrin-1 expression began to increase.Real-time PCR revealed that the expression level of netrin-1 mRNA was 37.59±10.25 times higher than control group when TEV-1 cells were exposed to 50 ng/mL neogenin(P<0.01),and the same tendency was seen by using Western blot.MTT results showed that proliferation of TEV-1 cells was independent of neogenin.Meanwhile,apoptosis was significantly increased to(22.15±6.15)% at50 ng/mL neogenin and(6.55±0.25)% without neogenin(P<0.01).It is suggested that neogenin regulates proliferation and apoptosis of TEV-1 cells.And it can enhance the ability of TEV-1 ceils to express netrin-1 in a dose-dependent manner.Neogenin may play an important biological role in the normal human pregnancy and contribute to the physiological pregnancy process.
10. Association between hypothyroidism and sleep breathing disorders in patients with coronary heart disease
Guanqi ZHAO ; Xiao WANG ; Jingyao FAN ; Wei GONG ; Wen HAO ; Shenghui ZHOU ; Aobo LI ; Ruifeng GUO ; Han SHI ; Zexuan LI ; Shaoping NIE ; Yongxiang WEI
Chinese Journal of Internal Medicine 2018;57(8):571-575
Objective:
To explore the association between hypothyroidism and sleep breathing disorders in patients with coronary heart disease (CHD).
Methods:
A total of 784 patients with CHD were consecutively enrolled at the Emergency & Critical Care Center of Beijing Anzhen Hospital from June 2015 to May 2017. According to thyroid function test results, patients were divided into hypothyroidism group (79 cases) and non-hypothyroidism group (705 cases). All patients had undergone sleep monitoring. The sleep apnea status was compared between the two groups. Multivariate logistic regression and linear regression models were used to analyze the association between hypothyroidism and sleep breathing disorders in patients with CHD.
Results:
The proportion of females, mean body weight and body mass index in the hypothyroidism group were higher than those in the non-hypothyroidism group [26.6% vs.16.2%, (78.6±11.6) kg vs. (75.7±12.0) kg, (27.7±3.2) kg/m2 vs. (26.6±3.5) kg/m2, all