A rapid method was developed for the determination of 5 common fatty acids, palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid in industrial oleic acid based on ultra_performance convergence chromatography_mass spectrometry ( UPC_MS) . The sample was dissolved by n_hexane, followed by clean_up of extract using 0. 22 μm organic phase filter. The fatty acids were separated in 3 min on the column of Acquity UPC2 BEH 2_EP by gradient elution with carbon dioxide and methanol/acetonitrile (1∶1, V/V) system, and finally detected by MS detector in ESI- mode. Through the optimization of UPC2_MS condition, the reasonable linearity was achieved for all the analytes over the range of 0. 5-100 mg/L with the correlation coefficients ( R2 ) greater than 0. 9985. The recoveries for five fatty acids at three spiked levels were in the range from 89 . 3% to 106 . 67% with relative standard deviations of 0 . 8%-3 . 0%. The limits of detection for target compounds in the method ranged from 0. 07 mg/L to 0. 26 mg/L. The real sample analysis showed that this method was simple,fast and had a good separation effect. There was no need of derivatization for fatty acid samples. This work would provide a fast and effective detection method for UPC2 technology in oil related research field.

Objective to summarize the experience of the use of traditional Chinese and western medieine,seff-Quyu Tongqiao side with Western medicine in treatment of traumatic intracranial hematoma,and improve the clinical success rate of treatment. Methods The experience of traditional Chinese and western medicine in treatment of patients with traumatic intraeranial hematoma retrospectively analyzed in our hospital from 2006 to 2008. Results Intracranial hematoma of 40 cases disappeared completely shown in CT one month after treatment.Conclusion The use of traditional Chinese and western medicine in traumatic intracranial hematoma is effective in the treatment of treatment of traumatic intraeranial hematoma and can improve the success rate of treatment.

OBJECTIVE: To investigate the gene expression of Cancer-Testis Antigen SCP-1 in human nasopharyngeal carcinoma. METHOD: The SCP-1 mRNA were examined in the tumor tissues of 28 nasopharyngeal carcinoma cases and control pharynx vasitis tissues of 40 non-cancer cases by reverse transcriptase polymerase chain reaction. A randomized sample of positive expression of SCP-1 mRNA was extracted for DNA sequencing to examine the reliability of results. The correlation of the expression of SCP-1 mRNA with clinical data was analyzed by statistical method. RESULT: The expression frequency of SCP-1 mRNA was 14.3% (4/28) in nasopharyngeal carcinoma and zero (0/40) in control pharynx vasitis tissue of non-cancer cases (P < 0.05); The DNA sequencing confirmed that the product of reverse transcriptase polymerase chain reaction was truly the target gene; No significant relationship was found between the expression of SCP-1 mRNA and clinical characters, such as age, gender and serum anti-EBV level (P > 0.05). CONCLUSION SCP-1 mRNA is expressed in some nasopharyngeal carcinoma, suggesting that SCP-1 might be a new tumor target antigen of nasopharyngeal carcinoma.
Adult ; Antigens, Neoplasm ; genetics ; metabolism ; Carcinoma, Squamous Cell ; genetics ; immunology ; metabolism ; Case-Control Studies ; Female ; Humans ; Male ; Nasopharyngeal Neoplasms ; genetics ; immunology ; metabolism ; RNA, Messenger ; genetics ; metabolism