1.Semi-laminectomy procedures for the management of intradurai extramedullary tumors of the spinal cord
Liyong HUANG ; Guosheng ZHOU ; Shaomei LI
Chinese Journal of Postgraduates of Medicine 2008;31(14):25-26
Objective To study the feasibility of semi-laminectomy procedures for the management of intradural extramedullary tumors of the spinal cord.Method Retrospective analysis of 19 cases of in-tradural extramedullary tumors of the spinal cord were treated by the approach of semi-laminectomy.including 11 cases of neurilemmoma and 8 cases of meningioma.Results Seventeen cases of extraspinal tumors were totally resection,2 of dumbbell neurilemmomas underwent subtotal resection.No new neurologi-cal disfunetions as well as recurrence or comphcations were found in all cases after the operation.Conclu-sion Semi-laminectomy procedures for the management of intradural extramedullary tumors of the spinal cord is a mini-invasive procedure,and is beneficial to the stability of spine,with the advantages of short hos-pitalization time,slight postoperative reactions and less complications.
2.Study on Present Situation and Countermeasures of Skipping Classes among Medicos and Non-medicos in Guangzhou
Shihua HUANG ; Yuanrui WANG ; Shaomei PAN
Chinese Journal of Medical Education Research 2005;0(06):-
In order to understand deeply the present situation and countermeasures of skipping classes among undergraduates,this paper uses questionnaire investigation,mathematical statistics and logical analysis to study the medicos and non-medicos in Guangzhou.The result shows it is very important to pay more attention to education,improve the quality of teaching,and so on.
3.Comparative Study of the Pharmacokinetics of Borneolum Syntheticum and Borneol in Herbal Preparations
Tianlai HUANG ; Shaomei YE ; Weiping OU ; Suiqin MI
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
Objective To compare the pharmacokinetics of borneolum syntheticum(BS)and borneol in compound Danshen dropping pills in rats.Method Rats were administrated orally with BS or Danshen dropping pills containing equal amount of borneol in a single dose.The blood contents of borneol at a series of time points were assayed by GC-MS and GC-FID after administration.The drug-time curve was estimated by PK Solution 2.0 pharmacokinetic software(USA).Results The pharmacokinetic parameters are quite different between the single dose of BS and the borneol in the compound Danshen dropping pills:the velocity constants of absorption(2.630h-1),distribution(1.788 h-1)and elimination(0.273 h-1)of BS were much greater than those(1.635h-1,0.784h-1,0.116h-1)of the same quantity of borneol in compound Danshen dropping pills,while the Tmax(0.5 h)of former was shorter than that(0.75 h)in the latter,and the Cmax(16.8 ?g/mL)in the former was about half of the latter(31.02 ?g/mL).And,at the 18h after administration,the former blood concentration(0.043 ?g/mL)was approximately one tenth of the latter.Conclusion It is suggested that we should pay attention to the influence of other ingredients on the main objective component in the study of pharmacokinetics of compound traditional chinese drug.
4.Effect of Surgical Hand Antisepsis Methods on Hand Skin Conditions:A Comparative Analysis
Dong PANG ; Xiuxia ZHENG ; Shaomei SHANG ; Qian LU ; Jingxiong HUANG ; Hongbai WANG
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To compare three surgical hand preparations for their effects on hand skin conditions.METHODS Three surgical hand antisepsis methods were selected to compare their immediate antimicrobial(efficacies) and effect on skin conditions,including 1% chlorhexidine gluconate(CHG)and 61% ethanol(pre-surgical) waterless,scrubless hand preparation,iodophor surgical scrub with sponge,and iodophor surgical scrub with brush.RESULTS All of the three methods could achieve satisfying immediate reduction in the normal(bacterial)(flora) of the hands.The alcohol-based surgical hand rub method was gentler to skin than the other two.(CONCLUSIONS) The alcohol-based surgical hand rub is recommended for surgical hand antisepsis.
5.Thermodynamic analysis for synthesis of aspirin catalyzed by aluminum tripolyphosphate
Wei XIE ; Jing WANG ; Zengwei HUANG ; Dongping WEI ; Aiqun YUAN ; Shaomei MA
Chinese Journal of Biochemical Pharmaceutics 2015;(3):169-171,175
Objective To explore the feasibility using aluminum tripolyphosphate as the synthesis of aspirin model instead of sulfuric acid catalyst. Methods The thermodynamic functions of the reaction system of salicylic acid and acetic anhydride were calculated according to the Benson group contribution method and Joback group contribution method.The enthalpy change,entropy and Gibbs free energy along with the change of temperature as well as the influence of the molar rate of reactants on the equilibrium conversation rate were also studied.Results In the temperature range of 298.15 K to 358.15 K,the reaction enthalpy was less than zero,and was exothermic reaction,and increase of temperature was not conducive to the reaction.The improvement of mole ratio of salicylic acid and acetic anhydride was helpful to improve the equilibrium conversation rate.The theoretical conversion rate could reach 99.58% when the mole ratio of salicylic acid and acetic anhydride was 3.Conclusion From the viewpoint of thermodynamics, the reaction is practical and feasible.
6.Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency.
Yanfang ZHANG ; Ruikun DU ; Shaomei HUANG ; Tao ZHANG ; Jinliang LIU ; Bibo ZHU ; Hualin WANG ; Fei DENG ; Shengbo CAO
Chinese Journal of Virology 2016;32(2):150-155
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
Animals
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Antibodies, Viral
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immunology
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Encephalitis Virus, Japanese
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genetics
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immunology
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Encephalitis, Japanese
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immunology
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prevention & control
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virology
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Humans
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Japanese Encephalitis Vaccines
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administration & dosage
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genetics
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immunology
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Mice
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Mice, Inbred BALB C
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Sf9 Cells
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Vaccination
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Vaccines, Virus-Like Particle
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administration & dosage
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genetics
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immunology
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Viral Envelope Proteins
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administration & dosage
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genetics
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immunology
7.Causes and management of brain MRI lesion progression in children with tuberculous meningitis during anti-tuberculosis treatment
Chongqing Medicine 2024;53(4):560-564,570
Objective To investigate the causes and management of cranial magnetic resonance imaging(MRI)lesion progression during anti-tuberculosis treatment of tuberculous meningitis(TBM)in children.Methods The medical records of 17 children with TBM from February 2011 to May 2022 in this hospital were analyzed retrospectively,including the occurrence time,duration,clinical symptoms,cerebrospinal fluid re-sults,imaging characteristics,causes and treatment schemes.Results The mean duration of cranial MRI le-sion progression in 17 children with TBM after anti-tuberculosis treatment was(33.59±19.50)days;11 cases(64.71%)had no clinical symptoms.The progression of cranial MRI lesions was manifested as thickening of the original meningeal lesion compared with the previous one in 11 cases(64.71%),enlarging of the original lesion in the brain parenchyma in nine cases(52.94%),the original lesion disappeared in six cases(35.29%),and the appearance of a fresh lesion in one case(5.88%).The reasons identified were paradoxical response(PR)in 14 cases(82.35%),diagnosis of drug-resistant tuberculosis in one case(5.88%),association with i-soniazid metabolism type of rapid acetylation in one case(5.88%),and increase in foci due to unregulated an-ti-tuberculosis treatment in one case(5.88%).The treatment regimens for the 14 cases of conflicting reac-tions were:HRZE(H:isoniazid;R:rifampicin;E:ethambutol;Z:pyrazinamide)or HRZ in combination with or without linezolid,extended or additional glucocorticoids in nine cases,addition of levofloxacin in three cases,no specific therapy in five cases;The metabolic type of fast acetylated isoniazid was adjusted to 15 mg/kg in chil-dren.The children with drug-resistant tuberculosis were changed to drug-resistant regimen;Children with poor dependence did not change the treatment plan.Conclusion PR is the common reason for the progress of brain MRI lesions after TBM anti-tuberculosis treatment in children,and glucocorticoid treatment can promote the lesion absorption.
8.A novel homozygous mutation p.E25X in the HSD3B2 gene causing salt wasting 3β-hydroxysteroid dehydrogenases deficiency in a Chinese pubertal girl: a delayed diagnosis until recurrent ovary cysts.
Yonglan HUANG ; Jipeng ZHENG ; Ting XIE ; Qing XIAO ; Shaomei LU ; Xiuzhen LI ; Jing CHENG ; Lihe CHEN ; Li LIU
Chinese Journal of Pediatrics 2014;52(12):948-951
OBJECTIVE3β- hydroxysteroid dehydrogenase deficiency (3βHSD), a rare form of congenital adrenal hyperplasia (CAH) resulted from mutations in the HSD3B2 gene that impair steroidogenesis in both adrenals and gonads. We report clinical features and the results of HSD3B2 gene analysis of a Chinese pubertal girl with salt wasting 3βHSD deficiency.
METHODWe retrospectively reviewed clinical presentations and steroid profiles of the patient diagnosed in Guangzhou Women and Children's Medical Center in 2013. PCR and direct sequencing were used to identify any mutation in the HSD3B2 gene.
RESULTA 13-year-old girl was diagnosed as CAH after birth because of salt-wasting with mild clitorimegaly and then was treated with glucocorticoid replacement. Breast and pubic hair development were normal, and menarche occurred at 12 yr, followed by menstrual bleeding about every 45 days. In the last one year laparoscopic operation and ovariocentesis were performed one after another for recurrent ovary cysts. Under corticoid acetate therapy, ACTH 17.10 pmol/L (normal 0-10.12), testosterone 1.31 nmol/L (normal <0.7), dehydroepiandrosterone sulfate 13.30 µmol/L (normal 0.95 - 11.67), cortisol 720 nmol/L (normal 130-772.8), androstenedione, 17-hydroxyprogesterone and progesterone were normal. Estradiol 461 pmol/L, follicle-stimulating hormone 3.04 IU/L, luteinizing hormone 8.52 IU/L in follicular phase. A pelvic ultrasound showed lateral ovaries cysts (58 mm × 50 mm × 35 mm) and a midcycle-type endometrium. A novel nonsense mutation c.73G >T (p.E25X) was identified in HSD3B2 gene. The girl was homozygous and her mother was heterozygous, while her father was not identified with this mutation.
CONCLUSIONA classic 3βHSD deficiency is characterized by salt wasting and mild virilization in female. Ovary cysts may be the one of features of gonad phenotype indicating ovary 3βHSD deficiency. A novel homozygous mutation c.73G >T(p.E25X) was related to the classical phenotype.
17-alpha-Hydroxyprogesterone ; Adolescent ; Adrenal Hyperplasia, Congenital ; diagnosis ; genetics ; Androstenedione ; China ; Codon, Nonsense ; Delayed Diagnosis ; Female ; Follicle Stimulating Hormone ; Homozygote ; Humans ; Hydrocortisone ; Luteinizing Hormone ; Mutation ; genetics ; Ovarian Cysts ; genetics ; Progesterone Reductase ; genetics ; Recurrence ; Retrospective Studies
9.A multicenter,random,open,parallel controlled study on the efficacy and safety of ibuprofen arginate in treating rheumatoid arthritis and knee osteoarthritis
Xiaomei LENG ; Fengchun ZHANG ; Zhanguo LI ; Xuewu ZHANG ; Donghai WU ; Huiqiong ZHOU ; Lingyun SUN ; Xiuyan YANG ; Liuqin LIANG ; Jieruo GU ; Jianlin HUANG ; Xinghai HAN ; Dongbao ZHAO ; Shengming DAI ; Shaomei HAN ; Tao XU
Chinese Journal of Rheumatology 2009;13(3):175-177
Objective To compare the clinical efficacy of ibuprofen arginate,a new nonsteroidal antiinflammatory drug,with that of ibuprofen,in patients with rheumatoid arthritis or knee osteoarthritis and to evaluate the safety and tolerability of ibuprofen argihate.Methods This is a muhicenter,random,open,active comparator-controlled,parallel clinical trail in which 171 patients with rheumatoid arthritis or knee osteoarthritis were enrolled.Patients were randomized to 2 groups:400 mg of ibuprofen arginate three times daily and 400 mg of ibuprofen three times daily respectively.Clinical efficacy and safety were evaluated after 4-week treatment.Results Ibuprofen arginate,at dosages of 400 mg three times daily,had shown significant efficacy in relieving pain,tenderness and swelling of joints and there was no significant difference when compared to that of ibuprofen.There was no difference in clinical adverse effects between the two groups and no serious adverse effects were repofled.But ibuprofen arginate could initiate effectiveness more rapidly than ibuprofen in both rheumatoid arthritisand osteoarthritis patients.Conclusion Ibuprofen arginate has the same clinical efficacy and safety profiles as itmprofen in treating rheumatoid arthritis and osteoarthritis.However,its onset is more rapid than ibuprofen.