Objective To study the effect of curcumin on the expression of inflammatory factors in model rats with chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS) type Ⅲ.Methods The rats were randomly divided into following groups: sham-operated group(sham), CP/CPPS model group(model), curcumin 50 mg group(cur-50 mg) and curcumin 100 mg group(cur-100 mg) and p38 inhibitor group(SB203580).Drug was given to each treatment group for 12 days by intraperitoneal injection.The levels of TNF-α, p38, COX-2 mRNA was measured by Real-Time PCR.The expressions of TNF-α and COX-2 proteins were measured by immunohistochemistry.The levels of p38, p-p38 and NF-κB proteins were detected by Western blot.Results NF-κB, p-p38, TNF-α, COX-2 proteins and TNF-α, p38, COX-2 mRNA in model group were higher than those in the sham group (P<0.01);cur-100 mg group and SB203580 group could significantly relieve these change in the model group (P<0.01);COX-2 mRNA and protein in cur-100 mg group decreased more than that in SB203580 group;p-p38 was positively correlated with NF-κB in curcumin groups and SB203580 group compared with model group (P<0.01).Conclusions Curcumin can reduce the expression of inflammatory cytokines NF-κB, TNF-α, COX-2 and p-p38 in rats with CP/CPPS type Ⅲ.

Objective To investigate the expressions ofnucleostemin (NS) and p53 up-regulated apoptotic factor (PUMA) proteins in human gliomas and its clinical significance.Methods Sixty-two human gliomas,collected in our hospital from January 2011 to February 2013 and confirmed by pathology,were used in our study; according to the WHO grading,grade Ⅰ included 8 patients in the study group,grade Ⅱ 21 patients,grade Ⅲ 19 patients and grade Ⅳ 14 patients.Other 10 patients accepted decompressions in our hospital at the same time period were used as control group.The expressions of NS and PUMA proteins in the brain tissues were detected by immunohistochemical S-P method.Results The NS protein expression was significantly higher and PUMA protein expression was statistically lower in study group than those in the control group (P＜0.05).As compared with gliomas of grade Ⅰ and Ⅱ,gliomas of grade Ⅲ and Ⅳ had significantly higher NS protein expression and lower PUMA protein expression (P＜0.05).Conclusions The expression changes of NS and PUMA are closely associated with gliomas malignant degrees,and the two play important roles in the progression and proliferation of glioma cells.

Objective:To asscentain the 131I activity concentration in 131I treatment workplaces and to explore the method of estimating the internal dose to workers by air sampling and to analyze its influencing factors. Methods:Air sampling method was used to collect aerosols containing radioactivity in 10 randomly selected workplaces in Zhengzhou where 131I therapy was performed. Aactivity concentration of 131I in treatment workplace was measured for gamma emitters by gamma-ray spectrometry. The internal dose due to 131I inhalation was estimated based on measurement result and field investigation result. Results:The activity concentration of 131I in air samples from 19 subpacking rooms ranged from 0.087 to 570 Bq/m 3, with an average of (51.04 ± 128.58) Bq/m 3. Those from 11 wards ranged from 0.162 to 54.6 Bq/m 3, with an average of (7.97 ± 15.89) Bq/m 3. In terms of the work hours recommended by the national standard GBZ 129-2016 Specifications for individual monitoring of occupational internal exposure, the estimated annual effective dose to radiation workers due to the inhalation of 131I ranges from 0.002 to 10 mSv, with an average of (0.61 ± 1.80) mSv, below the dose limit specified in the national standards. Conclusions:The samples with high 131I activity concentration in nuclear medicine workplaces of 10 medical institutions selected in Zhengzhou are mostly distributed in tertiary class hospitals operating large amount of radionuclide with large numbers of thyroid cancer patients adimitted. The result ing internal dose to radiation workers cannot be ignored. Estimating the internal dose based on the measurement result of air samples has a large uncertainty.However, air sampling method can promptly detect radioactive contamination in case of abnormal events or accidents, providing early warning for workers to carry out dose measurement from external exposure and internal exposure assessment.

Objective: To evaluate the effects of casein phosphopeptide-amorphic calcium phosphate (CPP-ACP) on enamel erosion using an improved in situ experimental protocol. Methods: Forty-eight enamel blocks were prepared from fresh-extracted human premolars and further embedded in the acrylic resins. The present study was divided into 2 parts. In part 1 of the study, two volunteers were recruited to test the availability and safety of the in situ erosion protocol. Customized intraoral appliance was made with 4 reservoirs containing the specimens for each volunteer. For each intraoral applicance, 2 reservoirs were made with the openings and the other 2 remained intact. All volunteers were instructed to drink 150 ml cola within 5 min using the gargling method after placing appliances intraorally for 2 h. After erosion, the appliances were remained undisturbed intraorally for 1 h until the next erosive attack. The in situ erosion cycles were performed 4 times daily over 7 d. In part 2 of the study, forty specimens were randomly divided into 2 groups (n=20): CPP-ACP group and control group. The surfaces of specimens in CPP-ACP group were pretreated with CPP-ACP for 3 min before in situ erosion, whereas the specimens in control group were pretreated with deionized water for 3 min. For each intraoral applicance, 4 reservoirs were made with openings. Ten healthy volunteers were recruited and the above-mentioned in situ erosion protocol was applied to test the effects of CPP-ACP on enamel erosion. The surface microhardness and surface microstructure of the samples were examined before and after erosion in both parts of the study. The data were statistically analyzed using ANOVA and LSD tests. Data were considered statistically significant at a level of P<0.05. Results: Significant surface softening was observed in all specimens after erosion (P<0.001). The surface microhardness in the CPP-ACP group and control group were (198.8±23.2) and (152.4±42.1) HV, respectively (P=0.040). The specimens in the CPP-ACP group showed significantly fewer changes in surface microstructure compared with those in the control group. Conclusions Based on this in situ experimental protocol, short-time consuming of acid beverages would produce significant effects on the surface microhardness of the human enamel, whereas the application of CPP-ACP can increase the erosion resistance of the enamel.