Objective To observe the effects of acupuncture combined with traditional Chinese medicine on scores of TCM syndrome and Neck Pain Questionnaire (NPQ) in patients of cervical spondylosis with syndrome of cold locking the collaterals. Methods Ninety cases of patients were randomly divided into acupuncture combined with TCM group, TCM group, and acupuncture group, 30 cases in each group. The acupuncture combined with TCM group was given modified Guizhi plus Gegen decoction and electro acupuncture;the TCM group was given modified Guizhi plus Gegen decoction only;the acupuncture group was given electro acupuncture only. The scores of TCM syndrome and NPQ of the three groups were observed and analyzed comparatively. Results The deviation scores of TCM syndrome and NPQ before and after treatment of the acupuncture combined with TCM group were higher than the acupuncture group and the TCM group (P<0.05), but there was no statistical difference between the acupuncture group and the TCM group (P>0.05). Conclusion Acupuncture combined with traditional Chinese medicine can relieve clinical symptoms of patients with cervical spondylosis with syndrome of cold locking the collaterals, ease their cervical pain, and improve their cervical function.

Objective To explore the influence of patient-controlled epidural analgesia(PCEA) on incidence of postpartum depression after cesarean section. Methods 256 cases of singleton term primary cesarean delivery were randomly divided into the study group( 130 cases)and the control group( 126 cases).The study group retained analgesia pump connected with epidural catheter,analgesic liquid was injected.In the control group,epidural catheter was removed,and was given intramuscular injection of pethidine hydrochloride 100 mg 4～6 h after operation,repeated 6 h later,total dosage was 200 mg.Postoperative observation time was 48h.Plasma prolactin(PRL) concentration was determined by radioimmunoassay in prenatal,postnatal 24 h and 48 h.Edinburgh postnatal depression scale (EPDS) was used to assess depression state 6 weeks postpartum in the two groups.The incidence of postpartum depression was compared between two groups. Results The plasma PRL level in the study group was significantly higher than the control group 24 h,48 h postpartum.The incidence rate of depression in the study group was 6.15％,while was 16.67％ in the control group,the differences were statistically significant. Conclusions The application of PCEA after cesarean section can significantly reduce the incidence of postpartum depression,and has very important practical significance in preventing the incidence of postpartum depression.

ObJective To acknowledge the sleep status of infants and analyze the influencing factors of sleep problems in them. Methods The sleep status of 136 infants and its influencing factors were investigat-ed using questionnaires, the results underwent descriptive analysis, single-factor analysis and multifactor anal-ysis. Results Difficulty in falling asleep was the major sleep problems, then was frequent night awakening. The influencing factors included: family type, education degree of the parents, inhabited environment, the feed-ing mode after birth, the period of supplementary protein dietary addition, the times of taking food through the night, and the sleep position, the bed, the emotion when falling asleep, etc. Conclusions Sleep problems is ubiquity in infants,and the unproper child rearing behaviors is the major influencing factor. In order to prevent sleep problems in infants and improve the quality of sleep in them, the parents need health guidance.

Objective To study further the etiology of cerebral palsy(CP),and the clinical effects of early diagnoses and intervening treatments.Methods The causes,clinical typing and CT/MRI features of CP in 240 cases were analyzed.The patients with CP were treated with the drugs of trophic nerve,the channel pilot frequency,the laser transmission and the drugs penetrating skin by point combined with functional exercise attaining ect.Results Among 240 cases in the study,birth asphyxia was 44.6％ .The total effective rate was 87.1％ . Conclusion Most cases may be prevented by health care in perinatal period.The younger the age,the more satisfactory is the therapeutic effectiveness for treating CP.

Objective:To study the effect of calcium sodium phosphosilicate(CSP)on the enamel remineralization of primary teeth. Methods:30 extracted human healthy primary molars were collected.Each tooth was cut bucco-lingually and mesial-distally into 4 sections.The sections were assigned randomly into 4 groups(n =30).Demineralization and remineralization cyclic model was estab-lished by etching with 35% phosphate acid for 2 min and then treated by unexposure of the sample to nothing(control,group A),so-dium monofluorophosphat(MFP,group B),CSP(group C)and MFP +CSP(group D).The cyclic was repeated twice daily for 30 d. The enamel surface morphology was observed by SEM and the surface microhardness(SMH)was measured.Results:On the tenth day,squamous morphology was observed on the enamel surface of group A and that of group D appeared less demineralization.The SMH value of group D was significant higher than that of group A(P <0.05).On the thirtieth day,obvious demineralization was ob-served in group A.Group B,C and D appeared surface remineralization.The SMH value of group B,C and D was significant higher than that of group A(P <0.05).Highest SMH value was achieved in group D.Conclusion:CSP combined with fluoride is more ef-fective in the enamel remineralization of primary teeth than the single application of them.

AIM: To investigate anti-oxidative and mitochondria protective effects of resveratrol on focal cerebral ischemia-reperfusion injury in rats. METHODS: Middle cerebral artery occlusion(MACO) was used to induce focal cerebral ischemia-reperfusion model.After 24 h reperfusion,MDA,GSH,NO,LD content and SOD activity in brain homogenate were determined,MDA,GSH content and SOD,ATPase activity in mitochondria were also determined. RESULTS: Resveratrol significantly inhibited the increase in MDA,NO,LD content and the decrease in SOD activity and GSH content in brain after cerbral ischemia-reperfusion.Resveratrol also significantly inhibited the increase in MDA and the decrease in activity of SOD,Na~+-K~+-ATPase,Ca~(2+)-Mg~(2+)-ATPase in mitochondria. CONCLUSION: Resvertrol has protective effects on focal cerebral ischemia-reperfusion injury via attenuating cerebral oxygen free radical lipid peroxidation and protecting mitochondria.

Objective Constructing golden hamster neural tube cDNA library in order to detect new genes associated with development of neural tube and neural tube defects. Methods Total RNAs were isolated from neural tube using TRIZOL Reagent; Doublestranded cDNAs were synthesized from total RNAs using LD-PCR method; The dsc DNAs were digested by proteinase K and Sfi Ⅰ, less than 500?bp fragments were separated by CHROMA SPIN-400, and then the cDNAs were ligated to ? TriplEX-2 vector.After packaging,the neural tube cDNA library was constructed. Results The titer of neural tube cDNA library was 1.5?10 6?pfu/ml.The recombination rat was 99%.The average insert was larger than 1?kb.Conclusion Our successfully constructed cDNA library is a full-length library with high efficiency,and can be used to detect the genes related to development of neural tube and neural tube defects.

Objective To explore the value of ultrasound in diagnosis and classification of pilomatricoma.Methods Ultrasonic features of 63 cases with pilomatricoma confirmed by operation and pathology were analyzed and summarized retrospectively.Results According to if there was calcification in the tumor,pilomatricoma cases were classified into two groups(calcific group and non-calcific group).In addition,there were three subgroups in the calcific group based on the size and disposition of calcification:subgroup Ⅰ,completely echogenic mass with strong posterior acoustic shadowing in the subcutaneous layer (n=17,26.98%);subgroupⅡ,hypoechoic nodules with tiny calcification diffused distributed(n=15,23.80%);subgroup Ⅲ,hypoechoic nodules with large calcification(n=8,12.70%).Twenty-three cases (36.51%)were distributed into non-calcific group.Conclusions Ultrasonography is valuable for diagnosis and differential diagnosis of pilomatricoma,and it is an essential examination method for the tumor before operation.

ObjectiveTo investigate the value of strain ratio (SR) in the diagnosis of Hashimoto's thyroiditis.Methods 116 patients who had been diagnosed clinically as Hashimoto' s thyroiditis were enrolled in the study.Those patients were classified into four groups:the hyperthyroidism group,the euthyroidism group,the subclinical hypothyroidism group,and the clinical hypothyroidism group.50volunteers with normal thyroid functions were enrolled as a control group.SR of the thyroid and sternocleidomastoid muscle of same side was calculated.The correlation coefficient between the elasiticity SR and the serum thyroid stimulating hormone (TSH) was evaluated and their differences in the diagnosis of Hashimoto's thyroiditis were compared.ResultsThe Spearman's correlation coefficient between the SR and the serum TSH was 0.605,which was significant (P =0.000).The SR increased in an ascending order among the group with hyperthyroidism,the control group,the group with euthyroidism,the group with subclinical hypothyroidism,and the group with clinical hypothyroidism.ConclusionsElastography SR is valuable way for evaluating the progression of Hashimoto's thyroiditis.

The ratio of plasma aldosterone concentration to plasma renin activity (ARR) is a practical parameter in screening for primary aldosteronism (PA).However,variations of the cutoff value of ARR in different studies have been reported due to plenty of influential factors that may affect the secretions of renin and aldosterone. Lack of standardization of assays for ARR also makes direct comparisons among different studies difficult.The associated influential factors on ARR were introduced in this review.