Objective To evaluate the difference between gallbladder polys,gallbladder adenomas and gallbladder cancer by sonography.Methods The imaging features were analysed including 571 patients with gallbladder polys,30 with gallbladder adenomas,109 with gallbladder cancer.Results The lesion diameter,echogram,color signals,maximum velocity(Vmax) and the resistive index(RI) were difference between Polys,Adenomas and cancers of gallbladder.When RI withinlesions lower than 0.65 was adopted as diagnostic criteria of gallbladder cancer,the specificity of the ultra-sound in diagnosis of gallbladder cancer were respectively.Conclusion Color Doppler flow imaging was useful in the differential diagnosis of gallbladder polypoid lesions in combination with gray-scale sonography.

Objective To iavestigate the influence of two disinfectants methods on disintection efficiency and model precision of dental impression.Methods Alginate impressions,alginate and agar complex impressions and silicon rubber impressions were made according to a unified standard.Chlorinous disinfectant solution and acidic oxidative potential water were used to disinfect the infected impressions.The influence of two disinfectants on disinfection efficiency and model precision of dental impression was compared.Results Two disinfectants for 30 minutes had significant disinfection effect on these three kinds of impressions.The effective rate reached 99.48％.Chlorinous disinfectant solution bad no significant influence on the model precision of alginate impressions and silicon rubber impressions,but had significant influence on the model precision of alginate and agar complex impressions.Acidic oxidative potential water did not influence the model precision of the three dental impressions.Conclusions Acidic oxidative potential water can be used to disinfect the three dental impressions.Chlorinous disinfectant solution can be used to disinfect alginate impressions and silicon rubber impressions,but cannot be used to disinfect agar complex impressions.

Objective To highlight the role of physician's experience in physical examination for the early diagnosis of breast cancer.Methods Data of physical examination of mammary glands in 1785 female were analyzed and compare with the uhrasonography and mammngraphy results.Results Of the 31 breast cancers confirmed by pathologic examination after operation,2 cases were bilateral breast cancers and 1 was nonpalpable.9 of 41 cases which suspected by inspection were confirmed as breast cancers.The significant difference was found comparing with control group by X2 test (X2=100.5,P＜0.05).The 34 breast cancers were found in 2089 focus in palpation and there were 6 minimal cancers (diameter≤1.0 cm) among them.Some cancers were found in special location with no malignant clinical manifestation.The rate of missed diagnosis of ultrasonography and rnammography is 16.1% and 19.4%,respectively.Conclusions Physical examination plays an important role in the early detection of breast cancer and physician's experience is a major determinant for avoiding misdiagnosis.

Objective To observe the relationship between carotid stiffness and coronary angiography in elderly patients of coronary heart disease (CHD) complicated with hypertension.Methods The patients were divided into three groups.Coronary angiography was performed in 40 elderly patients of CHD complicated with hypertension.The carotid stiffness was measured by ultrasound.Results There were significant differences on hypertension in arterial stain,arterial distensibility and arterial stiffness.There was significant correlation between coronary angiography score and carotid stiffness among three groups(r=-0.353,-0.125,0.376,P<0.01).Conclusion There is close relationship between carotid atherosclerosis and coronary atherosclerosis in elderly patients of CHD complicated with hypertension.

Objective To investigate the relationship between carotid stiffness and coronary angiography in elderly patients with coronary heart disease(CHD). Methods Thirty-five elderly patients with CHD who underwent coronary angiography were enrolled in the study. The carotid stiffness was measured by ultrasound, the results were compared with those in hypertension group,hyperlipemia group and healthy elderly group. Results There were significant differences in carotid tensity (8.15 ± 1.54), arteriectasis ( 0.34 ± 0.07 ) and carotid stiffness ( 640.51 ± 150.98 ) of elderly patients with CHD compared to other groups (all P＜0.05 or P ＜ 0. 01 ). There was significant correlation between coronary angiography score and carotid stiffness in elderly patients of CHD(P＜0.05 or P＜0.01). Conclusions There is close relationship between carotid atherosclerosis and coronary atherosclerosis in elderly patients with CHD.

Objective To establish a quality standard for Jianpibushen granules .Methods The five main ingredients of the formulation :Astragalus membranaceus ,Salvia miltiorrhiza Bunge ,Codonopsis pilosula ,Citrus reticulata Blanco and Paeonia lactif lora Pall ,were identified by thin layer chromatography (TLC) respectively .The content of peoniflorin in Paeo-nia lactif lora was determined by high performance liquid chromatography (HPLC) .The separation was performed on Agilent Eclipse Plus C18 column(4 .6 mm × 250 mm ,5 μm) .The mobile phase was acetonitrile and 0 .1% potassium dihydrogen phos-phate solution for gradient elution .The flow rate was 1 ml/min ,the column temperature was 25 ℃ ,the UV detection was per-formed at 230 nm .Results The spots in TLC were clear without interference in negative control .A good linear relationship was shown within the range of 8 .676-277 .632 μg /ml for peoniflorin (r=0 .999 9) .Conclusion This method is simple ,accu-rate and reproducible ,and can be used as an effective quality control of Jianpibushen granules .

BACKGROUND:microRNA-26b(miR-26b)plays an important regulatory role in a variety of stem cell functions,but its effects on the biological properties of stem cells from human exfoliated deciduous teeth and human umbilical cord mesenchymal stem cells are unknown. OBJECTIVE:To investigate the effects of miR-26b on the proliferation,migration and osteogenic differentiation of stem cells from human exfoliated deciduous teeth and human umbilical cord mesenchymal stem cells. METHODS:Stem cells from human exfoliated deciduous teeth and human umbilical cord mesenchymal stem cells were cultured and identified.miR-26 mimics(experimental group)and miRNAs mimics control(control group)were used to transfect above mentioned two kinds of cells and construct overexpressed models for subsequent experiments.CCK-8 assay was applied to detect the proliferation ability of overexpressed miR-26b cells.Transwell and scratch assay were employed to analyze the migration ability of overexpressed miR-26b cells.RT-qPCR was utilized to examine the expression of osteogenic markers after osteogenic induction of overexpressed miR-26b cells. RESULTS AND CONCLUSION:(1)Transfection of miR-26b mimics increased miR-26b expression in the two kinds of cells and promoted the proliferation of stem cells from human exfoliated deciduous teeth,with no significant effect on the amplification of human umbilical cord mesenchymal stem cells.(2)Compared with the control group,the migration ability was enhanced after two types of cells overexpressing miR-26b.(3)miR-26b expression decreased during osteogenic differentiation of the two kinds of cells.(4)Compared with the control group,the levels of osteogenesis-related genes osteocalcin,osteopontin,alkaline phosphatase,and human type I collagen mRNA were downregulated after overexpression of miR-26b in the two kinds of cells.The results showed that overexpression of miR-26b promoted the proliferation and migration of stem cells from human exfoliated deciduous teeth and inhibited their osteogenic differentiation;it promoted the migration of human umbilical cord mesenchymal stem cells and inhibited their osteogenic differentiation,but had no significant effects on their proliferation.