Objective To study the development of medical imaging in Shandong province and identify existing problems. Methods 378 secondary hospitals and above were surveyed with questionnaires and other means for a general picture of their radiology departments. The survey covered medical imaging examinations, human resources and equipment configurations. Results The recent five years have found a rapid growth of a variety of medical imaging examinations, digital imaging in dominance, high-end equipments as the mainstream, sufficient staffing, shortage of high-level talents in these hospitals. Conclusion The medical imaging examinations should be regarded as justified and reasonable, the competence of hospital staff should be upgraded, and operation of medical imaging examinations should be normalized.

Objective To analyse the CT radiation dose statistically using the standardized radiation-dose-structured report (RDSR) of digital imaging and communications in medicine (DICOM). Methods Using the self-designed software, 1230 RDSR files about CT examination were obtained searching on the picture archiving and communication system ( PACS ) . The patient dose database was established by combination of the extracted relevant information with the scanned sites. The patients were divided into adult group (over 10 years) and child groups (0-1 year, 1-5 years, 5-10 years) according to the age. The average volume CT dose index ( CTDIvol ) and dose length product ( DLP) of all scans were recorded respectively, and then the effective dose ( E) was estimated. The DLP value at 75% quantile was calculated and compared with the diagnostic reference level ( DRL) . Results In adult group, CTDIvol and DLP values were moderately and positively correlated ( r=0?41 ) , the highest E was observed in upper abdominal enhanced scan, and the DLP value at 75% quantile was 60% higher than DRL. In child group, their CTDIvol in group of 5-10 years was greater than that in groups of 0-1 and 1-5 years ( t=2?42, 2?04, P<0?05);the DLP value was slightly and positively correlated with the age (r=0?16), while E was moderately and negatively correlated with the age ( r = -0?48 ) . Conclusions It is a simple and efficient method to use RDSR to obtain the radiation doses of patients. With the popularization of the new equipment and the application of regionalized medical platform, RDSR would become the main tool for the dosimetric level surveying and individual dose recording.

Objective:The experiment researched relation between applying p38MAPK inhibitor(SB203580) and pulmonary injury during CPB.Methods:18 pigs randomly distributed three groups(n=6). We perfused the lung of the second group with SB203580 during CPB. The first group(n=6) and the second group(n=6) are control group. Lung samples were collected. Activated forms of P38 were measured by Western blot. Pulmonary injury was determined by histology. Tissue water percentage was measured. Pulmonary function was measured.Results:Phospho-P38 of the first group were increased compared with both the second group and the third group(P

Objective To investigate the imaging appearances of esophageal tuberculosis.Methods 5 cases of esophageal tuberculosis proved by pathology were retrospectively analyzed.There were 2 male and 3 female patients aged between 24 and 36 years median age 29 years. All cases were undergone esophageal barium examination, CT scan and gastroscopy. The surgical resection of the tumor was complemented in one case.Results Esophageal barium contrast study showed: the location of lesions at upper, middle and low esophagus in 1,3 and 1 cases respectively. The X-ray features included filling defect in 2 cases, centripetal strictures in 2 cases, intraluminal barium patch in 1 case, extramural irregular fistulous formation in 1 case, mucosa destruction in 2 cases and mucosa flattened or disappeared in 3 cases. On chest CT, bronchiectasis in the posterior basal segment of left inferior lung in 1 case, enlargement of right hilum of lung and small nodule in the right chest wall in 1 case, irregular thickening in the wall of inferior oesophagus in 1 case, pleural adhesion in 1 case were showed, and the other two were normal. The accurate diagnosis was confirmed only in 2 patients preoperation.Conclusion Esophageal tuberculosis extent is not of imaging characteristics, its diagnosis should be in combination with clinical data.

Objective To investigate radiation dose to the patients undergoing interventional radiology and make radiation risk assessment.Methods Data was collected on 198 instances of 10 interventional radiology procedures by using Philips Allura Xper FD20 DSA, which was equipped with the transparent ionization chamber system in compliance with IEC 60601-2.Patient peak skin dose and effective dose were estimated.Results Cumulative fluoroscopy time was 2.1 - 80.9 min, and number of images monitored for PSD were above 1 Gy and 79 cases monitored for E were above 20 mSv.Conclusions Substantial number of cases exceeded the dose threshold for erythema.Due attention should be paid to radiation protection of patients.

Objective To quantitatively study the features of cerebromspinal fluid(CSF)flow dynamies in normal sella region in MRI with phase-contrast method.Methods Seventeen healthy volunteers were studied.The CSF flow image in sella region was positioned at the middle sagittal T1WI or T2WI image.This pulse sequence used a encoding velocity of 20 cm/s.The waveforms were analyzed for the maximum flow velocity,flow volume rate and the change of the figures.From the velocity and area measurements on the cine images,mean CSF flow was calculated in millimeters per second and milliliters per cardiac cycle.Results The normal CSF flow of the sella region had two directions which was downward(caudal)flow during thesystolic period and upward(cranial)flow during the diastolic period of the cardiac cycle.The downward and upward peak flow velocity,mean downward and upward flow velocity and mean flow velocity was(1.44±0.99)cm/s,(302.71±248.15)ms,(1.16±0.64)cm/s,(331.00±225.38)ms,(0.49±0.39)cm/s.(0.67±0.44)cm/s,(0.54±O.30)cm/s,respectively.The downward and upward peak flow volume rate.mean downward and upward flow volume rate and mean flow volume rate was (0.014±0.009)ml/s.(0.012±0.006)ml/s,(0.047±0.041)ml/s,(0.053±0.003)ml/s,(0.005±0.003)ml/s,(0.034±0.031)ml/s,respectively.The mean cycle was(775.25±173.06)ms.Conclusion Phase-contrast method in MRI cine is a noninvasive method to study the CSF flow in physiological and pathological conditions for determining the pattern,direction,speed and quantity of the CSF flow.Therefore it is better than other invasive research modalities and has an important value in clinical application.

Objective To evaluate diagnostic accuracy of image interpretation using viewboxes at different luminance with ALVIM statistical phantom (TRG).Methods A digital film of TRG image was obtained by exposing the phantom of TRG with Kodak DR 3000 using proper parameter.Viewboxes with three different luminance were selected from those used in daily work.The film of TRG was evaluated with these viewboxes of three different luminance by three different radiologists with experience of five,eight and sixteen years respectively.A grade scale of 5 was used to evaluate each row of signal and noise.According to the results and formula,the probabilities of true-positive [P (S/s)],false-positive [P (S/n)] and true answer(Pdet) were obtained. Results The three kinds of luminance used in viewboxes were: (3489 ± 256),(2231 ± 220),(816 ± 168) cd/m2.When the average luminance of viewbox was 3489 cd/m2,Pdet of 0.6,0.7,0.8 mm in bone substitute was 0.558±0.009,0.788±0.008,0.813±0.006,Pdet of0.9,1.0,1.2 mm in muscle substitute was 0.663 ± 0.010,0.750 ± 0.008,0.933 ± 0.005 ; When the average luminance of viewbox was 2231 cd/m2 ,Pdet of 0.6,0.7,0.8 mm in bone substitute was 0.525 ± 0.013,0.713 ± 0.013,0.775 ± 0.016,Pdet of 0.9,1.0,1.2 mm in muscle substitute was 0.613 ± 0.019,0.650 ± 0.013,0.850±0.019 ;When the average luminance of viewbox was 816 cd/m2 ,Pdet of 0.6,0.7,0.8 mm in bone substitute was 0.475 ± 0.022,0.550 ± 0.018,0.688±0.020,Pdet of 0.9,1.0,1.2 mm in muscle substitute was 0.550 ± 0.025,0.575 ± 0.021,0.725 ± 0.016. Viewboxes of different luminance were compared to one another by paired t-test.The results of comparison all had significant statistical differences (t = 5.057 and 4.681 ,P <0.05).Conclusions The image displayed by viewboxes at different luminance had significant influence on image interpretation. The higher the luminace of viewbox,the richer the information displayed,and the higher the accuracy of diagnosis.

Caenorhabditis elegans is a very important model organism in life sciences. C. elegans has been widely used in research on life sciences, especially in drug screening and the mechanism of drugs, thanks to some of their prominent characteristics, including a short life,short generation cycle, and easy culture and observation. Aging is a complex process, which is the result of multiple factors. There are mainly three types of anti-aging signal pathways in C. elegans, including insulin-insulin-like growth factor-1 signal pathway, diet-restricted signaling pathway and mitochondrial respiratory chain/ATP synthesis pathway. In this paper, we reviewed the aging models based on the above three signaling pathways and the progress in anti-aging drugs based on the above aging models. In addition, a number of C. elegans models of aging-related neurodegenerative diseases can be obtained by using transgenic or chemical mutagenesis. Thus, this paper reviewed the transgenic models of C. elegans associated with neurodegenerative diseases, including theα-synuclein transgenic model of Parkinson disease, theβ-amyloid deposition model of Alzheimer disease, and the polyQ of Huntington disease, and summa?rized the effective drugs based on the above disease models. This review will provide reference for the study of C. elegans in the future screening of anti-aging drugs and drug screening for the prevention and treatment of neurodegenerative diseases.

Objective To study the role of Bid in simulated ischemia/reperfusion injury in cultured neonatal rat cardiocytes and its possible mechanism.Methods A cell culture model of neonatal rat(born within 3 days) cardiocytes was used to establish simulated ischemia/reperfusion model.Unrelated siRNA or Bid specific siRNA was transfected into rat cardiocytes by Oligofectamine.The activation of Bid and caspase-8 was detected by Western blotting.The apoptotic rate of cardiocytes was determined by flow cytometry.Results Bid and caspase-8 of cardiocytes were activatied in simulated ischemia/reperfusion injury model.The activation of caspase-8 of cardiocytes in Bid-specific siRNA group decreased significantly compared with those in simulated(ischemia/reperfusion) group and unrelated siRNA group;the apoptotic rate of cardrocytes(7.4%) was lower than those in simulated ischemia/reperfusion group(51.2%) and unrelated siRNA group(48.7%)(P

The human resistin expression vector was constructed and transfected into cells to observe its effects on glucose uptake in adipocytes,cell proliferation and differentiation.The results suggested that human resistin impaired glucose uptake in adipoeytes via stimulating proliferation of preadipocytes and suppressing adipocyte differentiation.Metformin reversed the inhibition imposed by resistin on adipogenesis.