Objective To investigate the stress distribution of the femur after cemented prosthetic replacement in aged patients with comminuted intertrochanteric fracture and to analyze the difference of stress distribution between cemented long and short stem prosthesises.Methods A volunteer's left femur image data was obtained by spiral CT tomography.Then,the three-dimensional model of the femur was reconstructed with Mimics and Unigraphics modeling softwares.Based on the above analysis,we established three-dimensional modles of comminuted intertrochanteric fracture,long stem and short stem prothesises,and mantle layer of cement.Finally,we established the three-dimensional finite element model of these materials with software ABAQUS6.5,and carried out finite element analysis to the model in biomechanics.Results The stress disrilbution of the femur after cemented long and short stem prosthetic replacement didn't change significantly.The stress distribution of short stem between cement and prothetic stem were in the correlative area of distal end of the prothesis,and the lateral peak value was 21.3 MPa which exceeds the fatigue strength of bone cement;However,that of long stem were in the middle of medial interface and in the distal end of bilateral interface.There was no significant stress concentration zone in the mantle layer of cement which was used to reconstruct calcar femorale.Conclusion The stress distribution of the femur didn't change significantly after treating comminuted intertrochanteric fracture of aged patients with cemented long and short stem prosthesises.The cemented long stem prosthesises may be more suitable to treat these patients for its lower loosening probability.

To evaluate the validity of osteoarthritis model induced by bilateral ovariectomy in guinea pig, 32-month-old female guinea pigs were randomly divided into two groups: a sham operation group (control group) and an ovariectomized group (OVX group). The animals were killed 6 or 12 weeks after the operation and the degeneration of the knees were assessed microscopically and histologically by scanning electron microscope (SEM), transmission electron microscope (TEM) and light microscope. The serum levels of estrogen and gestone were detected by immune contest assay. The scoring of articular cartilage histopathology of tibial plateau was performed by histopathological examination. The blood serum levels of estrogen and gestone were decreased significantly in the OVX group as compared with the control group 6 or 12 weeks after the operation. Joint cartilage degeneration as detected by SEM and TEM could be found at the 6th week, but severe degenerative lesions were observed at the 12th week in the OVX group as compared with the control group (P<0.01). The histopathological score of articular cartilage in tibial plateau in OVX group was higher than that of control group, which was coincident with the changes of estrogen and the ultrastructure (P<0.01). The findings suggested that bilateral ovariectomy in guinea pig can induce the severe osteoarthritis that is similar to the aging-induced OA in human. Therefore, the model of the osteoarthritis by bilateral ovariectomy in guinea pig in this study is valid.

Objective To explore the relationship between CD146(MCAM),microvessel density(MVD)in renal cell carcinoma(RCC) and its clinic-pathology,and to explore their correlation with clinic-pathologic parameter of RCC. Methods Immunohistochemisty was employed to determine the expression of CD146 and MVD in 43 RCC tissues and 20 normal control renal tissues. Results The positive expression of CD146 in RCC(90.7 ％,39/43) was remarkably higher than that in normal renal tissue(30.0 ％,6/20)(x2=27.77,P＜0.05).The expression of CD146 was not correlated with the category of RCC (x2=1.37,all P ＞0.05),but had a significant correlation to(the tumor volume x2=7.57)clinical stage(r=0.62) and metastasis of RCC(x2=19.99,P＜0.05). The MVD of RCC [(78.00±23.10)/200HP]was significantly higher than that of normal renal tissue [(23.05±7.93)/200HP].The MVD of CD146 was not correlated with the tumor volume and category of RCC (t=1.33,t=1.46,au P＞ 0.05),but had a significant correlation to clinical stage and metastasis of RCC (t=2.37,t=2.10,P＜ 0.05). There was a positive correlation between expression of CD146 and MVD in RCC(r=0.74,P＜0.05). Conclusion The overexpression of CD146 in RCC has a significant relation with tumor angiogenesis.The expression of CD146 and angiogenesis might serve as an important indicator of the development, progress and metastasis of RCC.

Objective:A study was conducted to determine the expression of acetyl-coa carboxylase product of phosphorylation (P-ACC) and an enzyme called cyclooxygenase 2 (COX-2) in non-small cell lung cancer (NSCLC) tissue, as well as the relationship and correlations between tumor size, lymph node metastasis, clinical stage, and pathological type. Methods: Sixty-two patients with NSCLC lung cancer tissues were included in the patient group, whereas 20 patients who underwent lobectomy for other reasons and had normal lung tissues were included in the control group. Immunohistochemical streptavidin peroxidase method was used to detect the expression of P-ACC and COX-2 in lung cancer and normal lung tissues. Results:The positive expressions of P-ACC and COX-2 in NSCLC lung cancer and normal lung tissues were significantly different (P<0.05). In NSCLC tissues, the positive expression of P-ACC was significantly associated with tumor size (P<0.05), but was not significantly associated with lymph node metastasis, clinical stage, and pathological type. We found no correlation between the positive expression of COX-2 and tumor size, lymph node metasta-sis, clinical stage and pathological type. Further analysis revealed that the positive expression of P-ACC and COX-2 in NSCLC was sig-nificantly and negatively correlated (r=-2.37, P=0.032). Conclusion:The positive expression of COX-2 in NSCLC greatly increased compared with that of P-ACC, and a significantly negative correlation was observed between them. We propose that the positive expres-sion of P-ACC reduction may activate the positive expression of COX-2 and promote the occurrence, development, invasion, and metas-tasis of NSCLC.

To evaluate the validity of osteoarthritis model induced by bilateral ovariectomy in guinea pig, 32-month-old female guinea pigs were randomly divided into two groups: a sham operation group (control group) and an ovariectomized group (OVX group). The animals were killed 6 or 12 weeks after the operation and the degeneration of the knees were assessed microscopically and histologically by scanning electron microscope (SEM), transmission electron microscope (TEM) and light microscope. The serum levels of estrogen and gestone were detected by immune contest assay.The scoring of articular cartilage histopathology of tibial plateau was performed by histopathological examination. The blood serum levels of estrogen and gestone were decreased significantly in the OVX group as compared with the control group 6 or 12 weeks after the operation. Joint cartilage degeneration as detected by SEM and TEM could be found at the 6th week, but severe degenerative lesions were observed at the 12th week in the OVX group as compared with the control group (P＜0.01).The histopathological score of articular cartilage in tibial plateau in OVX group was higher than that of control group, which was coincident with the changes of estrogen and the ultrastructure (P＜0.01).The findings suggested that bilateral ovariectomy in guinea pig can induce the severe osteoarthritis that is similar to the aging-induced OA in human. Therefore, the model of the osteoarthritis by bilateral ovariectomy in guinea pig in this study is valid.

Objective To investigate the anatomical changes and dose distribution deviations of locally advanced nasopharyngeal carcinoma ( NPC ) patients during intensity-modulated radiation therapy ( IMRT) . Methods Weekly cone beam CT ( CBCT) was applied to investigate the anatomical changes of enrolled 18 patients with locally advanced NPC. Dosimetric deviations were investigated with new IMRT plans after 20 fractions. Results The volumes of gross tumor volume ( GTVnx ) and GTVnd were decreased by 3. 15% and 5. 67% weekly, and decreased by a total of 22. 03% and 39. 68%, respectiely. The volumes of left and right parotids were decreased by 4. 93% and 5. 26% weekly, and decreased by a total of 29. 60% and 31. 56%, respectively. The volumes of GTVnd and parotids showed significant retraction in the first four weeks of radiation, then the retraction slowed down. The D95 of PGTVnd in Plan 2 decreased by 2. 20% (t=2. 382, P <0. 05) compared that in the initial Plan 1, while there was no significant difference on the D95 of PGTVnx , PTV1 and PTV2 . The Dmean , D50 and V30 of the left and right parotids in Plan 2 increased by 7. 34%, 12. 68%, 10. 90% (t = -3. 376, -3. 738, -3. 679,P<0. 05), and 6. 13%, 11. 17%, 9. 72% (t= -2. 550, -2. 446, -2. 673, P<0. 05), respectively, compared with Plan 1. The Dmean of larynx increased by 8. 69% in Plan 2 compared with Plan 1 ( t = -3. 099, P <0. 05). The D95 of PGTVnd in Plan(1+2) increased by 1. 37% (t= -3. 555, P<0. 05) compared with Plan 2. The Dmean, D50 and V30 of the left and right parotids in Plan(1+2) were decreased by 2. 90%, 2. 73%, 4. 62% (t=3. 089, 2. 718,2. 705, P < 0. 05), and decreased by 3. 49%, 3. 44%, 3. 80%(t=2. 781, 2. 958,4. 275, P<0. 05), respectively, compared with Plan 1. The Dmean of larynx decreased by 3. 29% (t=2. 747, P<0. 05) in Plan(1+2) compared with Plan 1. Conclusions The volumes of GTV and parotids of locally advanced NPC patients showed significant shrinks during IMRT. Replanning is necessary during the middle of IMRT to improve the target coverage and to spare the organ at risks ( OARs) .

Objective To understand and analyze the effect and impact on Hospital-level Young Scholar Scientific Research Program in personal training and project promoting.Methods To review and analyze the archived documents and data materials of total 402 Young Scholar Scientific Research Program involving 339 young fellows from 2003 to 2013,compared the research input and output with general hospital level,including getting new fund,publications,Awards and patent.Results Output on researchers with Young Scholar Scientific Research Program is much higher than the general hospital level,but showing unbalanced distribution in medical disciplines.Conclusions To establishment of Young Scholar Scientific Research Program has achieved very good effect in promoting research in tertiary public hospital as well as has empowered the young fellows for conducting scientific research independently.It is proposed to go on strength the scientific management and optimize the system construction of research input,further establishing academic communication platform for young fellows.

Objective The study reviewed the exchanges and the outcome of the research cooperation of the platform of the Beijing International Cooperation Base for Science and Technology (ICBST),to explore a combined mode of internationalized research management of "base","talents" and "projects".Methods To analyze the outcomes of talents programs,international research cooperation projects and publications with the support of the Beijing ICBST.Results The establishment of BICBST has nurtured research talents to develop international vision,facilitated project-oriented international research cooperation,and encouraged high level research publication and grants.Conclusions ICBST is of great significance for the implementation of international research cooperation,which brought in more resources,cultivated a group of creative research talents,and broadened the channels for international collaborations and achieved capacity and influence of international competitiveness.

OBJECTIVETo investigate the gene mutation frequencies and patterns of β-thalassemia (β-thal) in the minority populations of Guizhou province.

METHODSThree thousand and five hundred couples in the reproductive age were screened by using automatic hemocyte analyzer and hemoglobin autoanalyzer-variant. The diagnostic criteria for β-thal were: the mean corpuscular volume (MCV) was ≤ 82 fl, and the HbA(2) level was ≥ 3.5%. A total of 194 positive samples were detected and further identified by PCR-reverse dot blot (PCR-RDB) assay for 18 common β -thal mutations in Chinese population. Those subjects with positive phenotypes but without the 18 common β-thal mutations were subjected to DNA sequence analysis of the β-globin gene.

RESULTSOne hundred and eighty-nine samples with gene mutations were observed from the 3500 samples, with the incidence of β-thal being 5.4%. A total of 10 different β-thal mutations were identified from the 189 diagnosed samples. The five most common mutations were as the following: CD17 (43.9%), CD41-42 (38.6%), IVS-II-654(10.1%), -28 (2.6%) and CD71-72 (1.6%). In addition, a novel β-globin gene mutation (-CD53) allele was detected. One rare mutation of IntM was observed.

CONCLUSIONThe minority population in Guizhou province is of high risk of β-thal. It is recommended that more attention should be paid to detect the carriers of β-thal in the population in reproductive age by hematologic screening and common gene diagnosis in the area with high risk of β-thal.

Adult ; Base Sequence ; China ; ethnology ; DNA Mutational Analysis ; Ethnic Groups ; genetics ; Female ; Humans ; Male ; Middle Aged ; Mutation ; Young Adult ; beta-Globins ; genetics ; beta-Thalassemia ; genetics

Arthroplasty, Replacement, Knee ; adverse effects ; Female ; Humans ; Knee Joint ; diagnostic imaging ; surgery ; Middle Aged ; Radiography