Objective To study the distribution and antimicrobial activity of marine actinomycetes from Jiaozhou Bay and to estimate its application potentials.Methods The strains were isolated using gradient dilution and streak plate techniques and the activity was determined by Oxford cup method and paper disk method.Results 516 strains of marine actinomycetes were isolated from various places along Jiaozhou Bay;The rates of active strains determined by both methods were 22.3% and 50.0%,respectively.Conclusion The estuary was rich in marine actinomycetes resources; paper disk method was a better method in screening active strains of marine actinomycetes.The results from both determination methods showed a good exploitability of the marine actinomycets from Jiaozhou Bay.

Objective:In order to detect blood oxygen saturation timely,accurately,and conveniently,a wireless blood oxygen saturation detection module has been developed.Methods:Based on wearable monitoring technology and ZigBee wireless communication technology,we designed a blood oxygen saturation module of finger cuff type.This system is composed of photoplethysmography detection micromodule,ZigBee access terminal,and data analysis program,which realize the functions of waveform display,SpO2 calculation,wireless date transmission and so on.Results:The results show that detection accuracy of photoplethysmography is 98%,and calculating error of SpO2 is less than 8.8% satisfying the performance index of blood oxygen saturation detection.Furthermore,ZigBee wireless communication technology and light source regulator can reduce energy expenditure effectively.Conclusion:With the characters of wireless,low power consumption,miniaturization,and high performance,it can truly reflect physiological condition of patients,and remove lines on them,which make it possible for mobile monitor.

Phonocardiogram contains a lot of information on the physiological and pathological cardiac function, so it can improve the diagnosis and treatment of cardiovascular disease to effectively diagnose cardiac sounds. This paper presents the design of a computer-based measurement and real-time analysis system of heart sounds. Applying computers to the analysis and auxiliary diagnosis of cardiac sounds, the system is real-time and intelligent.

Objective To: analyze the operator learning curve of prostatic artery embolization (PAE) in treatment of prostatic hyperplasia (PH). Methods: Data of the first to 60th PH patients treated with PAE by the same operator having more than 5 years' experiences of peripheral vascular interventional therapy were retrospectively analyzed. The patients were divided into groups A, B and C according to the treatment order (each n=20). PAE operation time, intraoperative X-ray exposure time and post-operation hospitalization were compared among groups. International prostate symptom score (IPSS) and quality of life score (QOL), prostate volume, maximum flow rate (Qmax) and post void residual (PVR) before and after operation were analyzed. Results: There was no statistical difference of prostate volume, IPSS score, QOL score, Qmax nor PVR among three groups before PAE (all P>0.05).There were significantly statistical differences of operation time and intraoperative X-ray exposure time (F=32.74, 13.57, both P<0.01) among 3 groups. Pairwise comparison showed the operation time and intraoperative X-ray exposure time in group A were significantly longer than those in group B (both P<0.01) and group C (both P<0.01), while the differences between group B and C were not statistical (P=0.22, 0.30). No statistical difference of post-operation hospitalization was found among 3 groups (F=0.17, P=0.84). Three months after PAE, IPSS score (t=14.66, 11.74, 29.02), QOL score (t=8.51, 8.19, 7.99), PVR (t=11.68, 12.71, 16.80) and prostate volume (t=14.75, 17.45, 27.42) decreased compared with those before operation in all 3 groups (all P<0.01), and Qmax (t=-11.72, -10.80, -7.74) also statistically increased (all P<0.01). Conclusion: PAE is effective for PH in short-term. After accumulating 20 cases of PAE operation experience, the operation time and X-ray exposure are significantly shortened, and the learning curve turn to the plateau stage.

Objective To investigate the rituximab plus bendamustine (R-Benda) therapeutic effect for relapsed extragastric mucosa-associated lymphoid tissue (MALT) lymphoma.Methods Ten patients (three females and seven males) with relapsed extragastric MALT lymphoma undergoing therapy with R-Benda were defined.Bendamustine was given at a dose of 90 mg/m2 on days 2 and 3 in nine patients and at 70 mg/m2 in one patient, while all received 375 mg/m2 rituximab on day 1.Results Nine patients received six courses of therapy,while one patient discontinued therapy after five courses for personal reasons, while one elderly patient had progressive disease after three courses.Tolerance of therapy was excellent, and all except one patient responded.Eight patients achieved the complete remission, one achieved the partial remission, and one patient progressed.Toxicities were mild and mainly hematological.After a median follow-up of 24 months (range, 5-43 months), 9 patients were alive.Conclusion R-Benda regime has high activity and good tolerance for patients with relapsed extragastric MALT lymphoma.

OBJECTIVE:To provide the support for pharmacists in their clinical pharmaceutical care.METHODS:The hospital pharmaceutical care system was set up by employing Visual FoxPro6.0and developing series of database according to the characteristics of clinical pharmacy.RESULTS&CONCLUSION:This system has the characteristics of convenience,flexibility,and fastness,which can result in more scientific and accurate pharmaceutical care.

Acute-On-Chronic Liver Failure ; diagnosis ; Humans

ObjectiveTo investigate the clinical application of ultrasound-guided mammotome surgery in breast tumor.Methods579 breast tumors from 273 patients underwent minimally invasive surgery by ultrasoundguided mammotome system.ResultsThrough 0.5cm incisal opening,all tumors were exactly,completely resected and no infaust surgery complication happened.ConclusionThe way of minimally invasive surgery of breast tumor by ultrasound-guided mammotome system was simple,precise,safe,minimally invasive and was accurate diagnosis.So popularizing this minimally invasive techniques was deserved.

Objective To investigate the changes of serum homocysteine and it s relationship with oxidative stress and diabetic microangiopathy.Methods Eighty health participants were recruited as control 100 type 2 diabetis patients without diabetic microangiopathy were recruited as DM group,100 type 2 diabetis patients with diabetic nephropathy were recruited as DN group,and 100 type 2 diabetis patients with diabetic retinopathy were recruited as DR group.Their serum levels of homocysteine,malonaldehyde(MDA),superoxide dismutase (SOD)and glutathione(GSH)were measured.Results The homocysteine was(98.86 ± 21.46),(198.95 ±19.35),(138.65 ± 15.25)ng/L in the DM,DN and DR group respectively,which were signigicantly higher than that of(62.48 ± 15.36)ng/L in the control group(F =7.95,P ＜ 0.01).MDA was(17.49 ± 1.64),(22.47 ± 1.86)and(22.47 ± 1.86)mmol/L,which was significantly higher than that of(11.86 ± 0.48)mmol/L in the control group(F =6.89,P ＜0.01).The homocysteine and MDA in the DN and DR group were both significantly higher than those in the DM group(P ＜ 0.01).The SOD and GSH was(107.80 ± 15.62)mg/L and(179.26 ± 25.8)mg/L in the DM group,(79.86 ± 14.63)mg/L and(143.36 ± 21.75)mg/L in the DN group,(89.34 ± 12.75)mg/L and(156.96 ± 19.35)mg/L in the DR group,which were significantly higher than those of(128.32 ± 19.21)mg/L and(237.38 ± 27.31)mg/L in the control group(F =7.89 and 8.76 respectively,P＜0.01).The SOD and GSH in the DN and DR group were both significantly lower than those in the DM group(P ＜ 0.01),and the DN group was significantly lower than the DR group(P ＜ 0.01)Serum homocysteine was positively correlated with MDA(r =0.79,P ＜ 0.05),and negtively correlated with SOD and GSH(r =-0.71 and-0.78,P ＜0.01).Conclusion Diabetic microangiopathy patients have higher serum homocysteine level and severe oxidative stress.Oxidative stress were related to higher serum homocysteine level.The higher serum homocysteine level and oxidative stress might play an important role in development of diabetic Microangiopathy