The prevalence of obesity has become one of the most problems that affect human health in 21th Century.The offspring of obese mothers have a higher rate of perinatal morbidity and an increased risk of long-term health problems.Maternal obesity has been associated with high risk of adverse pregnancy outcomes such as stillbirth,congenital malformation of fetus,and an increased rate of cesarean delivery,especially in emergency cesarean section.The notion that an abnormal maternal metabolic environment,through epigenetic modifications,may lead to permanent changes in key organs that underlie fetal/juvenile programming of adult disease,such as obesity,cardiovascular disease,type Ⅱ diabetes and other metabolic syndrome.Therefore,by the weight control of obese gestational age women or the prevention of excessive weight gain during pregnancy,it will significantly decrease the affection of obese mothers on the fetus,reduce the occurrence of adult obesity,diabetes,cardiovascular disease and metabolic syndrome.

Objective:To observe changes of cerebral nerve cell apoptosis of the rat after acute stroke and the preventive and treating effect of Jiannao Decoction on the apoptosis.Methods:SHRsp rats were used to establish the model of acute stroke,which were treated with Jiannao Decoction.Symptoms and signs of the nervous system and changes of pathological structures of the hipocampus were investigated before and after establishment of the model,and changes of cerebral nerve cell apoptosis before and after establishment of the model and medication were investigated with TUNEL,immunohistochamical and Western blot methods. Results:The incidence rate of stroke in the prevention group was significantly lower than that of the model group(P0.05).Conclusion:Jiannao Decoction has significant protective action on brain in the rat with stroke,which is related with inhibiting the effect of nerve cell apoptosis and conduction of apoptosis-related signals.

0.05). The body surface area of recipients was positively correlated and the ratio of allograft weight to body surface area negatively correlated with the mean sCr in the 2～7 days after transplantation( P

Objective To observe the clinical efficacy of reinforcing-reducing needling methods in treating peripheral facial paralysis. Method Seventy facial paralysis patients were randomized into two groups to compare the reinforcing-reducing needling methods and conventional acupuncture. The House-Brackmann (H-B) scale was observed and compared prior to the treatment, right after the treatment, and respectively after 30-day, 3-month, and 6-month treatments, and the clinical efficacy was evaluated after 6-month treatments. Result The H-B score of the reinforcing-reducing manipulation group was superior to that of the conventional group (P<0.05), and the therapeutic efficacy of the manipulation group was more significant than that of the conventional group (P<0.05). Conclusion The reinforcing-reducing needling methods can produce a better therapeutic efficacy in treating peripheral facial paralysis compared to conventional acupuncture.

Objective:Describe the prevalence of depressive disorder and the care-seeking of depressed individuals among inpatients in general hospitals in Beijing.Methods:2 925 inpatients ≥15 years of age from 40 general hospitals (selected by stratified random sampling) were first screened by psychiatric nurses and then psychiatrists used DSM-IV criteria to generate a 'gold standard' diagnosis in subjects screened positive and 10% of those screened negative. Results:Adjusted point prevalence, one-year prevalence, and lifetime prevalence of any type of depressive disorder were 7.04%, 7.47% and 9.96%, respectively; corresponding prevalences of Major Depressive Disorder were 3.83%, 4.33%, and 6.11%, respectively. Of the 123 depressed patients only 12 (9.8%) had ever received treatment. The prevalence was significantly higher in females than in males(5.18%vs 3.59%,RR=1.44 95%CI:1.01~2.05), in patients from Level-2 hospitals than in those from Level-3 hospitals(6.28%vs 3.31%,RR=1.90 95%CI:1.31~2.75), and in patients on internal medicine wards than in those from surgery (6.08%vs3.60%,RR=1.69 95%CI:1.15~2.49)or other wards(6.08%vs 3.01%,RR=2.02 95%CI:1.21~3.37).Conclusions:The prevalence of depressive disorder among inpatients in Beijing's general hospitals is high but very few are diagnosed or treated. Different programs to increase the recognition and treatment of depression in general hospitals need to be developed and regularly monitored.

Objective To study the diagnosis and treatment of multiple-ligament injury to the knee and report the clinical outcomes of the disease.Methods A total of 51 patients with multiple-ligament tear in the knee were enrolled in this study.The lesion involved the ACL and MCL in 21 cases,ACL and PLC in 1,PCL and MCL in 4,PCL and PLC in 6,ACL,PCL,and MCL in 6,ACL,PCL,and PLC in 3,ACL and PCL in 8,and PCL and patella tendon in 2.Among the patients,14 patients had acute Ⅲ grade MCL tear and 3 had acute Ⅲ grade PLC injury;open suture repair were performed on these cases,and then arthroscopic surgery was carried out for other injured ligaments after 3-week immobilization.For 2 patients who had chronic Ⅲ grade MCL injury and 3 cases that were suffering from chronic Ⅲ grade PLC tear,open surgery for ligament reconstruction was employed,arthroscopy were used afterwards to remodel the ACL and PCL.In the 51 patients,totally 39 ACLs were injured,20 of these ligaments were reconstructed under a arthroscope using Hamstring tendon,17 were repaired with BPB by arthroscopy,and 2 were remodelled by implanting allogeneic tendons.In addition,29 PCLs tear were diagnosed,among which arthroscopic PCL reconstruction with autologous Hamstring tendon was performed on 19,allogeneic ligaments were used in 2,and the other 8 injured PCL were not repaied surgically because the lesion was relieved after immobilization(improved from grade Ⅲ to

Objective To study the developmental patterns and aging characteristics of anterior sacral foramina and posterior sacral forami-na in adolescents aging from 12 to 18 years old through CT imaging scanning and three-dimensional reconstruction measurement,and to provide the anatomical basis for the diagnosis and treatment of sacral vertebra diseases.Methods The 30 adolescents without any the problems of nerv-ous system were selected to conduct 64-slice spiral CT in the pelvic cavity.The original data were input into three-dimensional reconstruction software Mimics with the form of DICOM,and the relevant indicators were measured and analyzed.Results There was no significant difference in the height,width and distance of inner edge of anterior sacral foramina and the posterior sacral foramina in different gender and sides (P >0.05).The physical development of human increases gradually with age and there were significant differences.Conclusion There is a regular change process in the relevant indicators of anterior sacral foramina and posterior sacral foramina in adolescents.But the clinical appli-cation must be combined with the results of individual imaging,the technology of reverse engineering and the support of rapid prototype manu-facturing so as to meet the satisfactory results of individualized treatment.

Ergosterol is an economically important metabolite produced by yeast. To improve the production of ergosterol by Saccharomyces cerevisiae YEH56 (pHXA42) from molasses, a cheap and regenerative material, different strategies were applied. First, Plackett-Burman design and central composite design were applied to screen the significant factors in fermentation medium using ergosterol yield (g/L) as the response value. Ergosterol yield reached 371.56 mg/L by using the optimal fermentation medium in shake-flask culture (total sugar in molasses 40 g/L, KH2PO4 1 g/L, K2HPO4 1.86 g/L, CuSO4 x 5H2O 17.5 mg/L, FeSO4 x 7H2O 13.9 mg/L, MgSO4 x 5H2O 12.3 mg/L, corn steep liquor 10 mL/L), which was increased by 29.5% compared with the initial culture. Second, ergosterol yield was increased by 62.1% using a pH-control strategy in a 5-L bioreactor. Third, ergosterol production was improved further by using molasses feeding strategy. After 38 h fermentation, ergosterol yield reached 1 953.85 mg/L, which was 3.2 times of that in batch fermentation. Meanwhile, ergosterol production rate was increased by 42.7% compared with that in the batch culture.
Culture Media ; Ergosterol ; biosynthesis ; Fermentation ; Industrial Microbiology ; Molasses ; Saccharomyces cerevisiae ; genetics ; growth & development ; metabolism

Objective To discuss the treatment strategy of knee dislocation and to evaluate its outcome.Methods Thirty-six patients with unilateral knee dislocation treated with individualized protocol were studied retrospectively,including 22 males and 14 females with an average age of 35 years (range,19-72 years).There were 30 acute and 6 chronic knee dislocations.According to the modified Schenck's classification of knee dislocation,there were 7 KD- Ⅰ and 23 KD-Ⅲ cases in the acute category,and all 6 chronic cases were KD-Ⅲ.Seven acute KD- Ⅰ and 6 chronic KD-Ⅲ cases underwent one-stage arthroscopic surgery.In 23 acute KD-Ⅲ cases,2 were treated conservatively with splint or brace due to advanced age,18 with staged surgery,3 with one-stage surgery due to concomitant vascular and nerve injury.Functional and clinical evaluation was conducted at final follow-up.Results All 36 patients were followed up for an average of 27 months (range,18-36 months).The Lysholm score (82.0±11.4),Tegner score (5.5±1.3),and knee range of motion (118.3°±19.2°) at final follow-up showed a statistically significant improvement compared with preoperative results (P＜O.O1).Eight (23.5％) patients had residual knee instability:posterior drawer test and Sag sign were positive (++ or +++) in 8 knees,valgus instability (++) in 1 and varus instability (+++) in 1.The remaining 28 knees were stable.Conclusion Special attention should be paid to rotational knee dislocation with single cruciate ligament rupture.Properly individualized treatment plan is the key to optimal outcome.Arthroscopic surgery can lead to successful outcome.Early one-stage arthroscopic surgery is recommended for acute KD-Ⅰ and chronic KD-Ⅲ dislocation,staged arthroscopic surgery for acute KD-Ⅲ dislocation.

Objective To observe the anti-inflammation effect of antidromic electronic stimulation of rat dorsal roots. Methods Cross-excitation between adjacent primary afferent fibers and subcutaneous injection of 2% FM were adopted here as two inflammatory models. The firing rate and mechanical threshold of single units after cross-excitation were collected. The phosphorylated cAMP response element binding protein (p-CREB) level in spinal cord induced by formalin was visualized with immunohistology. Results Antidromic electronic stimulation (ADES) of dorsal roots could significantly inhibit the p-CREB over expression to FM injected in the contralateral hindpaw, and the increase in firing rate and mechanical threshold induced by cross-excitation were also inhibited by the ADES. Conclusion Antidromic electronic stimulation of the dorsal root may produce a systemic anti-inflamma-tion capacity.