Objective To explore the best way and the security of hypertrophic scar treatment by surgery combined with isotopic 90 Sr-90 Y or triamcinolone acetonide.Methods Hypertrophic scar of rabbit ears were divided into direct treatment group (Group A received 90 Sr-90Y irradiation,Group D received triamcinolone acetonide),two days 90 Sr-90 Y irradiation after surgery repair group (Group B),one week treatment after surgery repair group (Group C received 90Sr-90 Y irradiation,Group E received triamcinolone acetonide),saline injection group (Group F received saline directly,Group G received saline injection after surgical repair one week later),Group H received nothing,and then the histological changes were compared in each group.Results The 90Sr-90 Y exposure groups (Groups A,B,C) and triamcinolone acetonide injection groups (Groups D and E),the number of fibroblasts,collagen fiber surface density,microvessel counts and α-SMA-positive particle optical density values were significantly lower than the saline groups (Groups F,G) and blank control group.The most obvious group was Group B; Groups A,C,D,and E were not statistically significant.The number of melanoma cells of 90Sr-90Y exposure group (Groups A,B,and C) was significantly lower than other groups,with statistically significant difference.Atypical cells were not found in 90Sr-90 Y applicator exposure group.Conclusions Isotope 90 Sr-90 Y and triamcinolone acetonide inhibits the proliferation of the rabbit ear hypertrophic scar,the scar tissue fibrosis is reduced significantly,but there is no significant difference between the two effects.Early isotope 90 Sr-90 Y intervention on hypertrophic scar can achieve better results.Hypertrophic scar by isotope 90 Sr-90 Y radiation is easy to cause depigmentation,but generally does not cause cancer.

Forensic medical talents with creative ability should have ability to find.analyze and solve problems from complex cases.According to teaching practice on the basis of PBL this article illustrates application methods of PBL teaching characters in the training of forensic medical talents with creative ability.

Objective To explore the influence of preoperative chemotherapy (POCT) on telomerase activity(TA) of breast cancer and its mechanism. Methods TA was detected in 40 primary breast cancer specimens. Forty patients were divided into two groups: (1)POCT group. 32 patients, who underwent POCT using CMF scheme; (2) control group . 8 patients, who did not receive any POCT or radiotherapy. Results TA in POCT group(TA=0.499) was significantly lower than that in control group (TA=1.018). TA in patients received one , two or three times of POCT was 0.812,0.492 and 0.280 respectively. The difference of TA in patients received 1 time POCT and receive ≥ 2 times POCT was significant (P

Objective To investigate the expression and its rule of transferrin (Tf) in brain tissue at different time after experimental intracerebral hemorrhage (ICH) in rats. Methods The fresh quantitative autologous blood was infused into the right caudate nucleus of rat sterotaxically to build up experimental ICH model. At 6h, 24h, 72h and 7d after operation, the rats were sacrificed and the brain tissues were made for immunohistochemistry analysis of Tf. The water content of the brains was also assayed.Results Compared with saline group, Tf-immunostaining positive cells in the tissue surrounding hematoma and in the ipsilateral pallium in ICH rats were increased significantly during 7 days, peaked at 72 hours ( P

Objective To investigate the expression of neuron nitric oxide synthase (nNOS) mRNA of brain tissue at early stage of intracerebral hemorrhage (ICH) in rats and the intervention effect of Didangtang.Methods 72 male Sprague-Dawley rats were randomly divided into four groups: intracerebral hemorrhage group, Didangtang treatment group, NOS inhibition group and normal saline group. Quantitate fresh autologous blood was infused into right caudate nucleus of rat sterotaxically to build up experimental ICH model and normal saline was instead in control group. The neurological function deficit scores were observed by Bederson method (3 grades) at 6 h, 24 h and 72 h after operation. At the same time points, the rats were sacrificed and the brain tissues were taken out for the measurement of nNOS mRNA by technique of hybridization in situ.Results Neurological function deficit scores of the rats were significantly improved both in intracerebral hemorrhage group and Didangtang treatment group at 72 h after operation(all P

0.05).(4) There was significant correlation between CD62p and CD63 ( P 0.05). Conclusions Patients with cerebral infarction show evidence of enhanced platelet activation and increasment of MPV and MAR,which may relate to the pathological process of cerebral infarction. Moreover MPV and MAR can reflect the clinical severity of cerebral infarction better than PLT. Anti-platelet aggregation treatment should be further taken in patients with cerebral infarction.

0.05), but they did at 48 h(P

The changes of retinal nuclear DNA content in rats after death was detected and the relationship between degradation of retinal nuclear DNA and postmortem interval (PMI) was analyzed. Ninety healthy adult SD rats, female, weighing 250+/-10 g, were randomly divided into 15 groups. At 20 degrees C, the retinal cells were withdrawn every 2 h within 0 to 28 h after death and stained with Feulgen-Vans. Index of density (ID), integral absorbance (IA) and average absorbance (AA) in retinal nucleus were analyzed by image analysis system. And the obtained data were subjected to linear regression analysis by using SPSS12.0 software. The results showed that in retinal nucleus, AA and IA were gradually declined with the prolongation of PMI, while ID had an increased tendency. Within 28 h after PMI, the regression equations were as follows: Y(AA )=-0.009X(AA )+0.590 (R(2)=0.949), Y(IA )=-0.097X(IA )+18.903 (R(2)=0.968), Y(ID)=0.122X(ID)+2.246 (R(2)=0.951). It was concluded that retinal nuclear DNA after death in rats was degraded gradually and had a good correlation with PMI.

AIM:To investigate the interplay between the neuropeptide Y(NPY) and renin-angiotensin system, and relationship to the pathogenesis of hypertension. METHODS: The method of cellular culture, MTT colorimetric assay and quantitative immunocytochemistry through ACAS570 were performed for the effect of neuropeptide Y on proliferation of cultured rat vascular smooth muscle cell (VSMC) and losartan treatment. RESULTS:It was observed that exposure of VSMC to neuropeptide Y could stimulate the proliferation of VSMC and caused increase respectively in MTT OD values and expression of proliferating cell nulear antigen(PCNA) but losartan interfered with NPY stimulating effects on VSMC and decreased MTT OD values and expression of PCNA. CONCLUSION:These results demonstrated that the NPY could promote proliferation of VSMC, this effect was partly preformed through angiotensin Ⅱ receptor.

AIM: To observe expression of proliferating cell nuclear antigen (PCNA), platelet derived growth factor (PDGF) and c-myc in cultured vascular smooth muscle cells which is effected by neuropeptide Y (NPY) and losartan (the receptor blocking agent of angiotensin Ⅱ) therefore exploring effects of NPY on the generation of hypertension and its relationship with losartan reverse treatment in molecular biology. METHODS: Applied the method of quantitative immunocytochemistry through ACAS570. RESULTS: 24 hours exposure of vascular smooth muscle cell to NPY caused an increase in expression of PCNA, PDGF and c-myc respectively. But losartan could reverse these expressions by NPY, decreased the expression of PCNA, PDGF and c-myc. CONCLUSION: NPY is closely related to the generation of hypertension. But losartan could reverse these effects of NPY.