1.Seroreversion of serological tests for syphilis in neonates born to mothers with syphilis: a follow-up study
Xianghui LI ; Fang YANG ; Shaofeng HUANG ; Dongping LU ; Rong ZHANG ; Baoqing DENG
Chinese Journal of Dermatology 2012;(12):851-854
Objective To observe the seroreversion of serological tests for syphilis in neonates born to mothers with syphilis and to evaluate the influence of antisyphilitic treatment on the time of seroreversion in uninfected neonates.Methods A total of 115 neonates born to mothers with syphilis were recruited in this study,and underwent toluidine red unheated serum test (TRUST),Treponema pallidum particle assay (TPPA)and 19S IgM-TPPA at birth.Follow up was scheduled at 1,3,6,9,12,18 and 24 months of age,and cancelled as soon as both TRUST and TPPA became negative during the 24 months.Both TRUST and TPPA were carried out at each follow-up visit.Kaplan-Meiter survival analysis and Wilcoxon rank-sum test were conducted to assess these data by using SPSS 13.0 software.Results At birth,TPPA was positive in all (100%) of the neonates,TRUST in 97 neonates (84.3%) with the titer ranging from 1 ∶ 1 to 1 ∶ 256,19S IgM-TPPA in 3 neonates who were diagnosed with congenital syphilis (CS).CS was ruled out in the other 112 neonates with negative 19S IgM-TPPA after follow up.Of the 3 neonates with CS,all became negative for TRUST within 12-18 months after birth,2 remained positive for TPPA at 24 months of age,1 remained positive for TPPA at 20 months of age,and all were still followed at the time of this writing.The cumulative seroreversion rate was 82.2%,98.9% and 100%for TRUST at 0-3,4-6 and 7-9 months of age,respectively in 90 uninfected neonates,3.1%,30.2%,85.4% and 100% for TPPA at 0-3,4-6,7-9 and 10-12 months of age,respectively in 96 uninfected neonates.Of 16 neonates who missed some follow-up visits,all turned negative for TRUST and TPPA within 15 months after birth.As far as the TP-seropositive uninfected neonates were concerned,no statistical differences were observed in the seroreversion time of TRUST or TPPA between neonates receiving antisyphilitic treatment and those without antisyphilitic treatment (x2 =0.54,2.41,respectively,both P > 0.05).The seroreversion of TRUST occurred earlier than that of TPPA (Z =10.45,P < 0.01).Conclusions Most uninfected neonates born to syphilitic mothers turn negative for TRUST within 6 months after birth,and for TPPA within 12 months.And the seroreversion of TRUST usually occurs earlier than that of TPPA.Antisyphilitic treatment shows no significant influence on the seroreversion time of TRUST or TPPA in these seropositive uninfected infants.
2.IDENTIFICATION OF BACTERIUM D-97 PRODUCING TREHALOSE FROM STARCH OR MALTOOLIGOSACCHRIRIDES
Shaofeng RONG ; Ji XU ; Haiping ZHANG ; Jing YANG ; Zuo DUAN ; Zhonggui MAO ;
Microbiology 1992;0(02):-
A bacterium D 97 whose enducellular enzymes can produce trehalose from starch or maltooligosaccharides was isolated from Datian in Northeast of China The morphological, Cultural and Physiological Characteristics of trehalose prodicing bacterium D 97 were described in this paper There are galactose and lysine in its cell wall but no diaminopimelic acid (DAP) arabinose The content of G+C mol% is 61 3 The full lenth of 16s rDNA (770bp) has been tested and compared with the type strains of all known Arthrobacter sp in Genebank, the results showed the similarity values of 16s rDNA sequence between bacterium D 97 and Arthrobacter nicotinovorus were 97 98%, therefore bacterium D 97 was nameed Arthrobacter nicotinovorus D 97 In this paper we also carried out some comparisons of Physiological Characteristics between Arthrobacter nicotinovorus D 97 and Arthrobacter sp Q36
3.The reliability of the Chinese version of the kinesthetic and visual imagery questionnaire among stroke patients
Hua LIU ; Jun ZHOU ; Shaofeng YANG ; Qingzhao SHI ; Yuqi CHEN ; Yang LI ; Xiangjiang RONG ; Tong ZHANG
Chinese Journal of Physical Medicine and Rehabilitation 2017;39(4):259-263
Objective To develop kinesthetic and visual imagery questionnaires applicable to Chinese stroke survivors and evaluate their test-retest reliability,inter-rater reliability and internal consistency.Methods The English version of a kinesthetic and visual imagery questionnaire (KVIQ) was translated into Chinese using wellaccepted questionnaire translation procedures.Thirty stroke survivors were each assessed twice using two versions of the translation (the KVIQ-20 and the KVIQ-10) by two experienced raters with an interval of 7 days between the tests.The test-retest reliability,inter-rater reliability and internal consistency of the visual imagery score (visual imagery subscale),the kinesthetic imagery score (kinesthetic imagery subscale) and the total scores for the KVIQ-20 and KVIQ-10 versions were analyzed.Results The test-retest coefficients,the inter-rater reliability correlation coefficients and Cronbach's alphas for the KVIQ-20 version ranged from 0.879 to 0.945,from 0.894 to 0.936 and from 0.867 to 0.919,respectively.The corresponding measurements for the KVIQ-10 vcrsion were 0.914 to 0.953,0.852 to 0.900 and 0.827 to 0.878.Conclusion Both the KVIQ-20 and KVIQ-10 Chinese instruments have shown good testretest and inter-rater reliability and good internal consistency in assessing stroke survivors.Either is an effective tool for assessing their motor imagery ability.