The Guangdong Province Laboratory Animals Ordinance(the GPLAO),officially enacted and implemented in October 2010,marked a new phase of legal management in the administration of laboratory animals in Guangdong Province.The GPLAO clearly authorizes the laboratory animal monitoring institutes in Guangdong Province to conduct laboratory animal quality monitoring and assume corresponding legal responsibilities.It also specifies the standards for the production and use of laboratory animals,while bringing attention to laboratory animal welfare and ethics.Through the formulation of supporting documents,the active promotion of the standardization and regulation of administrative law enforcement,and the implementation of measures to delegate power,streamline administration,and optimize government services,significant improvements have been made in the standardized management of laboratory animals in Guangdong Province and in supporting the innovative development of biomedicine.Moreover,notable achievements have been made in areas such as the construction of experimental animal technology platforms,standardization work,the preservation and development of unique resources,and talent pool development.However,with the advancements in science and technology,social economy,and legislative work,some aspects in the GPLAO have been identified for improvement.These include the need for clearer provisions on laboratory animal welfare,more specific guidelines for the management of genetically engineered animals,improved regulations on the safety and welfare of practitioners,further refined implementation of the"one-vote veto system",continued promotion of laboratory animal standardization,and the exploration of effective mechanisms for resource sharing.This paper reviews and summarizes the institutional characteristics,post-legislation implementation,and effectiveness of the GPLAO,as well as the issues and countermeasures identified during the implementation.It concludes the laboratory animals management practices in Guangdong Province during the past 10 years,aiming to provide a reference for laboratory animal legislation.

Objective:To explore arboviruses carried by midges in Shizong county, Yunnan province.Methods:A total of 74 batches of 3705 midges collected from Shizong county, Yunnan province in July 2013 were detected by RT-PCR method, and then the amplified bands were cloned and sequenced. MegAlign in DNAStar software was used for homology analysis, MEGAX software ALIGN for sequence alignment and genetic evolution analysis based on Maximum Likelihood (ML) method .Results:Among 74 batches of midge samples collected in Yunnan province, 600 bp electrophoresis bands were amplified in 3 batches: SZC33, SZC42 and SZ625C8-2, while no electrophoresis bands were amplified in the other 71 batches of midge samples. After cloning, we selected 5 clones for sequencing, among which the 5 clones of sample SZC42 obtained 589 nt sequences. BLASTx comparison showed that the 5 cloned sequences of SZC42 had the highest amino acid homology(69%) with the non-structural protein gene of Culex bastrovirus-like(CAVL) virus found in mosquitoes collected in the United States, which was designated as Midge bastrovirus-like virus (MAVL). The result of genetic evolution analysis showed that MAVL detected in midges formed a single evolutionary branch, and it’s homology of nucleotides and amino acids with other astrovirus(AV) was less than 71.4%. Further analysis revealed that MAVL had a close genetic evolution relationship with CAVL (NC_040647) detected in American mosquitoes, AV (MF042208) detected in Brazilian sewage and AV (NC_032426) detected from Vietnamese bats, and the homology was 61.4%-66.2% (nt) and 67.7%-71.4% (aa), while far genetic evolution and low homology of nucleotides and amino acids with other AV sirains.Conclusions:A new astrovirus (MAVL) was detected in midges collected from Shizong county, Yunnan province in 2013.