ObjectiveTo explore the possible mechanism that Gadd45a and Egr-1together participate in the DNA damage repair,by analyzing the influence of hepatic ischemia-reperfusion injury (IRI) on the rnRNA and protein expression of hepatic cell cycle-related genes.Method Methods Forty-two SD rats were divided into normal control group,ischemia 30 min group and reperfusion 30 min,1h,2 h,3 h and 24 h groups.The hepatic specimens of rats at the corresponding time points after success of modeling were obtained. Chip experiment was used to screen out the key genes Gadd45a,Egr-1and GAPDH for target genes.We used Northern blot and RT-PCR to confirm the chip's results. The expression of related proteins was detected by using immunofluorescence and immunohistochemistry.Results Northern blot detection results showed that the RT-PCR products and the target genes for testing were concordant.The expression spectral chip signal and RT-PCR detection revealed that the expression of target genes had a 4-fold increase at early reperfusion period (1h) (up-regulation of Egr-1,Gadd45a and HSP70-1a by 9.39,8.28 and10.8 respectively.At the later stage of reperfusion (24 h),the expression of Egr-1,Gadd45a and Hspala was reduced to the normal levels, and the chip screening results were consistent with the RT-PCR test results.Fluorescence immunohistochemistry demonstrated that in the reperfusion1h and 2 h groups,the expression of Egr-1in nuclei of liver cells was positive. Immunohistochemistry indicated that the Gadd45 expression in the nucleus and cytoplasm was gradually increased with increasing reperfusion time.ConclusionGadd45a and Egr-1are the key genes of the nuclear factor (NF)-kB regulatory pathways,and involved in the regulation of cell cycle arrest at G2/M phase and repair of damaged DNA in rat hepatic IRI.

Eosinophils ; immunology ; Food Hypersensitivity ; etiology ; Gastritis ; etiology ; Humans ; Infant ; Male ; Milk Hypersensitivity ; complications ; immunology ; Milk Proteins ; immunology

Objective:To provide some suggestions for improving the pharmaceutical advertisement supervision system and standardizing the pharmaceutical advertisement market. Methods: Literature reviews, statistical analysis and interviews were adopted to study the status quo and difficulties of pharmaceutical advertisement supervision and pharmaceutical advertisement trends. Results:The top-level design of pharmaceutical advertisements supervision sys-tem has two defects:the separation between approval and penalty and the obstacles to cooperation at all levels of the drug administration. The worsening status quo of illegal drug advertising has elicited concern from scholars and regu-lators. However, there are still some difficulties in the supervision of drug advertisers, advertising agents, and pub-lishers. Conclusion:It is suggested that the drug advertising regulation system should be perfected by strengthening information supervision, establishing a coordination mechanism and consolidating self-discipline consciousness. Addi-tionally, laws and regulations should be promptly revised as an essential way to improve the effects of supervision.

Objective To find the features of cryo-injured infarcted model and the therapeutic effects of skeletal satellites cells(SCs) on myocardial infarction(MI).Methods SCs were implanted into the center of cryo-injured myocardium.Immunohistochemistry,confocal immunofluorescence double-labelling staining and transmission electron microscopy were used to study the state of SCs.Results After cryo-injured frontal wall of the left ventricle(LV) for approximately(15 s) by aluminium rod with(5 mm) in diameter,it made 43% weight of LV ischemia and 17% weight of LV infarcted.At 2nd week,injured area occured as transmural infarct.After transplantation,the implanted cells survived in large numbers.The transplanted cells proliferated remarkably at early phase and differentiated into many multinuclear myotubes after 2 weeks.Some of them developed into muscle fibers with better sarcomeres,but the gap junction was not formed between implanted cells and cardiac myocytes.Conclusions Cryo-injured infarcted model is a proper model to study myocardial infarction.Skelatal satellite cell transplantation will repair morphological structure partly of myocardial infarction area.

OBJECTIVE:To know the utilization of essential medicines in outpatient department of Sichuan Shuangliu Maternal and Child-care Hospital,and provide reference for the rational drug use. METHODS:The types,names,formulations,consump-tion sum and utilization of essential medicines in the outpatient department of the hospital from 2012 to 2013 were statistically ana-lyzed and ranked. The affordability of top 10 drugs in the list of DDDs was evaluated. RESULTS:Compared with 2012,the con-sumption sum proportion of essential medicines in the outpatient department of the hospital in 2013 was increased by 5.80%. The top 10 essential medicines in the list of consumption sum were mainly anti-microbial drugs,anti-anemia drugs and Chinese patent medicines. The relative daily wages of Kangfuxin solution and Amoxillin and clavulanate potassium tablets were respectively 2.12 d and 2.05 d,with poor affordability,and in 2012,they ranked the 5th and 8th in the list of DDDs;the relative daily wages of Xiao-er changweikang granule and Xingpi yanger granule were respectively 2.28 d and 2.14 d,with poor affordability,and in 2013, they ranked the 8th and 10th in the list of DDDs. CONCLUSIONS:The utilization of essential medicines in the hospital is basical-ly good,with a trend of increasing. However,the similar essential medicines with lower prices should be considered when essential medicines are used in the clinic.

The serum sialic acid level and its relation to stroke in syndrome X was observed in 21 syndrome X patients with history of stroke,21 syndrome X patients without history of stroke, 21 simple NIDDM patients and 21 healthy subjects matched for age, sex, duration of diabetes and HBAlc level.The results showed that the serum sialic acid level was significantly higher in all NIDDM patients than in normal subjects(P

This article analyzes the characteristics of lymph node metastasis in proximal advanced gastric cancer,in order to guide the extent of stomach resection (proximal or total gastrectomy) and the resection of spleen or splenic preservation. The metastasis of lymph node in proximal advanced gastric cancer has certain characteristics, which can provide a significant guidance for lymphadenectomy. Total gastrectomy plus splenectomy is a feasible radical surgery for proximal advanced gastric cancer, because of the requirement of lymphadenectomy.

Objective To observe the the therapcutic efficacy of differential antihypertensive treatments on the elderly acute cerebral hemorrhage with different amounts of bleeding. Methods 86 elderly patients with acute cerebral hemorrhage combined by hypertension were randomly divided into two groups:active antihypertensive group and regular antihypertensive group (n=43 for each group),among which 62 cases aged (68.9±5.3) years were in hemorrhage of small amount (volume ＜30 ml),24 cases aged (70.8 ± 5.7) years in large amount hemorrhage (volume ≥30 ml).The patients in active antihypertcnsive group received intravenous antihypertensive drugs within 1 hour of treatment in the following 7 d to reach systolic pressure 140-160 mm Hg.In contrast,in the control group the patients' systolic pressure reached 180-200 mm Hg at early stage.The hematoma volume was measured by CT before treatment and at 3 d and 7 d after treatment.NIH stroke scale (NIHSS)was used before treatment and 7 d and 28 d after treatment Results In the patients with small amount of bleeding,NIHSS scores and the total efficacy rate at 28 d after treatment and hematoma size at 3 d after treatment were better in active antihypertensive group than in regular antihypertensive group [(4.8±2.7) scores,86.67％ aud (2.4±0.6) ml vs.(7.6±3.9) scores,53.13％ and (8.1±3.1) ml,all P＜0.01]. However,for the patients with large amount of bleeding,there were no statistical differences in the above indexes between the two differential strategies (all P＞0.05).Conclusions Active antihypertensive therapy may inhibit early hematoma volume,improve prognosis and enhance clinical efficacy for the patients with small bleeding,but no clinical significance for the patients with large amount of bleeding.

The immune-gold-silver stain and image analysis methods were used to check up estrodial receptor (ER) in rat heart at myocardial infarction (MI) conditions.Animals were divided randomly into four groups: one serves as normal control, another as sham-operation, the third was treated as myocardial infarction and the last one estrogen (E_2) was injected after MI. The results showed that all the parts of the heart have ER,but the distributions are not equal in atrium and ventricle, the former is less than that of the latter (0.1687?0.005, 0.2072?0.006, P

OBJECTIVE:To discuss the role of licensed pharmacists in retail drug stores.METHODS:The current situation of licensed pharmacists’role playing in retail drug stores and the influcing factors were analysed.RESULTS&CONCLU?SION:The licensed pharmacist system remains to be perfect in China,we suggust that the licensed pharmacists'role should be further strengthed from two links,i.e.“access”and“employment”.