1.Case-control study on two osteotomy techniques for the treatment of distal radial malunion.
Bing-bing ZHANG ; Zhi-guo YUAN ; Jian-jun SHAO ; Shi-ning YANG ; Xi-ping CHAI
China Journal of Orthopaedics and Traumatology 2015;28(7):622-627
OBJECTIVERadial corrective osteotomy is an established but challenging treatment for distal radial malunion. There is an ongoing discussion about whether an opening or closing-wedge osteotomy between should employed. The purpose of the present study was to retrospectively compare the clinical and radio graphic results between conventional opening-wedge osteotomy and closing-wedge technique.
METHODSFrom January 2004 and December 2012,42 patients with extra-articular distal radial malunion were managed with corrective osteotomy and were followed for a minimum of one year. Twenty-two patients (5 males and 17 females, ranging in age from 25 to 75 years old) were managed with radial opening-wedge osteotomy and implanting of interpositional bone graft or bone-graft substitute, and twenty patients (4 males and 16 females, ranging in age from 19 to 79 years) were managed with simultaneous radial closing-wedge and ulnar shortening osteotomy without bone graft. The selection of the surgical procedure was determined by the surgeon. Each patient was evaluated on the basis of objective radio graphic measurements, and functional outcomes were determined on the basis of clinical examinations, including range of wrist motion, grip strength, pain-rating score, Mayo wrist score, and Disabilities of the Arm, Shoulder and Hand (DASH) score.
RESULTSThe mean duration of follow-up was 36 months (ranged, 12 to 101 months) for the opening-wedge cohort and 28 months (ranged, 12 to 87 months) for the closing-wedge cohort. The two techniques were comparable in terms of complications. Post-operative volar tilt and ulnar variance were improved significantly in each cohort. The ulnar variance was more frequently restored to within defined criteria (22.5 to 0.5 mm) in the closing-wedge cohort than that in the opening-wedge cohort. The post-operative mean extension-flexion are of the wrist and Mayo wrist score were significantly better in the closing-wedge cohort. Differences in the pronation-supination arc, grip strength, pain-rating score, and DASH scores between these two cohorts were not significant.
CONCLUSIONThe closing wedge osteotomy technique is an effective reconstructive procedure for the treatment of extra-articular distal radial malunion. It is significantly better than the opening-wedge osteotomy technique in terms of the restoration of ulnar variance, the extension-flexion arc of wrist motion, and the Mayo wrist score.
Adult ; Aged ; Bone Nails ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; Humans ; Male ; Middle Aged ; Osteotomy ; Radius Fractures ; surgery ; Range of Motion, Articular ; Retrospective Studies ; Wrist Joint ; surgery ; Young Adult
2.Expression, purification of Staphylococcus aureus Efb protein and preparation of its functional antibody
Xin, ZHANG ; Ya-Ping, GAO ; Jie, DONG ; Yu, LIU ; Na, WU ; Ning-Sheng, SHAO ; Guang, YANG
Bulletin of The Academy of Military Medical Sciences 2009;33(6):531-534
Objective:To clone and express the Staphylococcus aureus Efb(extracellular fibrinogen-binding protein) protein in Escherichia coli, to purify the expression product and prepare its functional antibody and to detect the functions of Efb protein for further studies on S.aureus infection.Methods: Efb gene was amplified by PCR using S.aureus NCTC-8325 genome DNA as template and cloned into the recombinant expression vectors pET28a. E.coli BL21(DE3) with the plasmid was induced with IPTG for protein production. The protein was purified by Ni~(2+) affinity chromatography. The function of Efb protein was determined by complement activity assay and inhibition ELISA.The polyclonal antibodies were prepared by immunizing the animals. Results: The purified recombinant Efb was obtained, which could inhibit the CH50 and AH50 effectively. The functional poly-antibodies of Efb were prepared.Conclusion:Efb could inhibit the classical pathway and alternative pathway of complement activation, and the antibodies against to Efb could block the inhibition of the classical pathway of complement activation induced by Efb.
3.Biosynthesis and assay of crude T-2 toxin
Yan-hong, CAO ; Shao-ping, WANG ; Yang, HUI ; Xiao-yan, FU ; Wei, DONG ; Ning, LIU
Chinese Journal of Endemiology 2008;27(5):552-554
Objective To isolate Fusarium species from Kashin-Beck disease(KBD)area and biosynthesize cnlde T-2 toxin.Methods T-2 toxin.producing Fusarium was isolated from corns produced in KBD area and purifted.The purifted funsi were identified according to the traits of colony,appearance of thallus and characters of conidium and then weIe cultivated in sterile Corn culture media.After extraction with organic solvent and purification by silica gel chromatography column,the quality and quantity of the toxin in the extracts were estimated by thin,Layer chromatography and high-performance liquid chromatography.Results The toxin-producing strain was Fusarium tricinctum. The com cuIture media inoculated with this strain produced about 250 mg of crude T-2 toxin per kg. Conclusions This experiment has indirectly further confirmed pollution of T-2 toxin-producing Fmarium existed in
4.Detection of early glial responses in the retina,optic nerve and optic chiasm in acute ocular hypertension rat model
Shao-dan, ZHANG ; Wei-yi, LI ; Wen-qian, WANG ; Ping, HUANG ; Ning-li, WANG ; Chun, ZHANG
Chinese Journal of Experimental Ophthalmology 2012;30(8):704-708
Background Glial cells perform specialized function in many aspects of the development,homeostasis,and function of neurons.Retinal ganglion cells (RGCs)and glia interactions are critically important in glaucomatous neurodegeneration.However,the precise mechanisms of glial activation and ganglion cells damage are still remained unclear. Objective This study was to assess the early responses of glial cells in the retina,optic nerve and optic chiasm in rat models of acute high intraocular pressure (IOP),and to examine the expression of nestin,a neuronal progenitor marker,in the reactive glias. Methods Acute high IOP of 110 mmHg was induced in the right eyes of 6 clean adult female Wistar rats by infusing normal saline solution into the anterior chamber for 60 minutes.Three normal matched Wistar rats were used as controls.The rats were sacrificed by overanaesthesia and sections of retina,optic nerve and optic chiasm were collected on 3 days and 7 days after the injection.Rat retina was examined by Nissl staining to illustrate the gross structure changes.Loss of axons of RGCs in the optic nerve was assessed by immunostaining of β Ⅲ-tubulin.Double labeling of glia] fibrillary acidic protein (GFAP) and nestin was performed in sections of retina,optic nerve and optic chiasm to evaluate the glial responses.The use of the animals complied with Statement of Animal Ethic Committee of Peking University Third Hospital. Results In control rats,GFAP-positive glial cells were observed in the retina,optic nerve and optic chiasm,where only weak positive response for nestin was noticed.Three days after acute IOP elevation,thickness of inner plexus form layer was significantlydecreased in comparison with the control rats.A loss of 46% RGCs was found in the rats with ocular hypertension.Obvious increase of GFAP expression was displayed in the retina,and processes of GFAP-positive glia cells extended into outer retina accompanied with significant up regulation of nestin.Axons in the optic nerve demonstrated a tendency of degeneration.Nestin expression increased significantly in the GFAP-positive glias in the optic nerve.Cross-sectional area of optic chiasm corresponding to the injured retina decreased relative to its countcrpart.Astrocyte like GFAP and nestin-colabeled glials were observed in this part of optic chiasm.The pathological changes of the retina,optic nerve and optic chiasm in hypertensive eyes aggravated on 7 days. Conclusions Acute ocular hypertension induce early onset of RGCs loss and axon degeneration.Neuronal injury is accompanied with glial reaction.Reactive glial cells express neuronal progenitor markers.The structural changes of the optic nerve and optic chiasm occur simultaneously with the high IOP.
5.A new method for quick analysis of tanshinone II(A) and salvianolic acid in Fufang Danshen tablets by AOTF-near infrared spectroscopy.
Ning WANG ; Shao-Song CAI ; Hong WEI ; Hai-Ping DONG
China Journal of Chinese Materia Medica 2008;33(3):261-264
OBJECTIVETo establish a new method using AOTF-Near infrared spectroscopy (NIRS) for quick determination of tanshinone II(A) and salvianolic acid B in Fufang Danshen tablets.
METHODHPLC was used as a reference method to determine the contents of tanshinone II(A) and salvianolic acid B in Fu Fang Dan Shen tablets. Multivariate calibration models based on PLS1 algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods.
RESULTRMSECV (root-mean-square error of cross-validation) of the models for tanshinone II(A) and salvianolic acid B were 0.0103 and 0.1868 respectively. The correlation coefficients of the calibration models were 0.9873 and 0.9832 respectively. External validation with external validation samples proved that the relative coefficient of the predicted value and the truth value were R2 = 0.9743 and R2 = 0.9886 respectively, with measured recycle rates of 103.0% and 99.0%.
CONCLUSIONNIRS can be used in the determination of tanshinone II(A) and salvianolic acid B, which sets up the foundation of product-line control of Fuang Danshen tablets.
Benzofurans ; analysis ; Diterpenes, Abietane ; Drugs, Chinese Herbal ; chemistry ; Phenanthrenes ; analysis ; Reproducibility of Results ; Spectroscopy, Near-Infrared ; methods ; Tablets ; chemistry
6.Study on the identification of HIV/AIDS and its surveillance system in rural areas of central China.
Jun WEI ; Na HE ; Shao-ping NING ; Shao-liang DONG ; Shao-xian JIA ; Mei-yang GAO ; Chao-wei FU ; Cheng-li LI
Chinese Journal of Epidemiology 2005;26(9):680-683
OBJECTIVETo explore the effective strategies for human immunodeficiency virus (HIV) surveillance and identification in rural areas of central China.
METHODSIn a selected rural prefecture area of central China, an epidemiological investigation was conducted for all reported HIV/AIDS cases. A historical and analytic review was performed, with particular interests in examining the HIV epidemic reporting and identification system.
RESULTSAmong all 626 reported HIV-infected individuals in the selected prefecture, 86.1% (539/626) of them were infected through commercial plasma donation or clinically operated blood transfusion. With respect to disease surveillance and identification in the area, 52.2% (327/626) of all the cases were reported by hospitals or clinics. The number and proportion of HIV/AIDS cases identified or reported at county, prefecture, and provincial levels were 207 (33.1%), 303 (48.4%) and 116 (18.5%), respectively. The number and proportion of HIV/AIDS identified through specific epidemiological investigations and/or voluntary testing had been increased in recent years. In addition, among HIV/AIDS cases that were clinically identified, the proportion of those who were identified as outpatients had steadily increased,with the highest proportion (59.3%) observed in 2004.
CONCLUSIONSIn rural areas of central China where the major mode of HIV transmission was through commercial plasma donation or clinically operated blood transfusion, hospitals and clinics seemed to have played and would continue to play important roles regarding HIV identification and surveillance. The role of institutions or settings at the prefecture level regarding HIV identification and surveillance should not be ignored.
Acquired Immunodeficiency Syndrome ; epidemiology ; transmission ; Adolescent ; Adult ; Aged ; Ambulatory Care ; statistics & numerical data ; Child ; Child, Preschool ; China ; epidemiology ; Demography ; Female ; Hospitalization ; statistics & numerical data ; Humans ; Male ; Middle Aged ; Rural Population ; statistics & numerical data ; Young Adult
7.Clonality analysis of multifocal bladder tumors.
Shao-bo ZHANG ; Yan-ping ZHANG ; Li-na LIU ; Yan-ning ZHANG ; Shou-fang HUANG ; Liang CHENG
Chinese Journal of Pathology 2007;36(1):5-6
Carcinoma, Small Cell
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genetics
;
pathology
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Carcinoma, Transitional Cell
;
genetics
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pathology
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Clone Cells
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DNA, Neoplasm
;
genetics
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Humans
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Loss of Heterozygosity
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Lymphatic Metastasis
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Microsatellite Repeats
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Urinary Bladder Neoplasms
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genetics
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pathology
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X Chromosome Inactivation
8.Postmortem MSCT diagnosis of whiplash injuries in a traffic accident: a case report and review of the literature.
Min CHEN ; Ping HUANG ; Lei WAN ; Jian-Hua ZHANG ; Ning-Guo LIU ; Dong-Hua ZOU ; Zheng-Dong LI ; Yu SHAO ; Zhi-Qiang QIN ; Yi-Jiu CHEN
Journal of Forensic Medicine 2014;30(2):148-150
A 45-year-old male car driver died in a traffic accident of four cars rear-end collision on the highway. He was found to have died after a respiratory and cardiac arrest at the scene. No sign of skin injuries was observed from the external inspection. The autopsy was not permitted by the family members because of the local culture. Multislice computed tomography (MSCT) was applied to the current case, showing dislocation of C3-4 cervical vertebrae with II degree, C4 vertebral plate fractures, and spinal stenosis. Post-mortem MSCT confirmed the diagnosis as whiplash injuries. MSCT was verified to be effective in showing the severity of whiplash injuries, thus providing certain objective evidence for medicolegal expertise.
Accidents, Traffic
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Autopsy
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Humans
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Male
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Middle Aged
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Multidetector Computed Tomography
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Spinal Fractures
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Whiplash Injuries/diagnostic imaging*
9.Severe hypokalemia due to combination use of dexamethasone and calcium gluconate for diabetics with infection: a case report.
Shun-qi HAN ; Zhi-qiang QIN ; Kai-fei DENG ; Jian-hua ZHANG ; Ning-guo LIU ; Dong-hua ZOU ; Zheng-dong LI ; Yu SHAO ; Ping HUANG ; Yi-jiu CHEN
Journal of Forensic Medicine 2014;30(4):304-306
The combination use of dexamethasone and calcium gluconate can be applied to hypersensitivity. Severe hypokalemia is a usual complication of dexamethasone and calcium gluconate therapy, which occurs frequently with therapeutic use. Fatal cases, accidental and intentional, occur frequently in forensic practice. The current case report presented a 43-year-old man with diabetes mellitus with infection, to whom dexamethasone and calcium gluconate were administered in the private clinic. With the development of such clinical symptoms of severe hypokalemia as quadriplegia, he was confirmed to have severe hypokalemia through a biochemical test before dying of arrhythmia. And also it presented pathophysiologic mechanism underlying severe hypokalemia as well as suggestions for clinical practice regarding combination use of dexamethasone and calcium gluconate.
Adult
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Anti-Inflammatory Agents/adverse effects*
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Calcium Gluconate/adverse effects*
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Dexamethasone/adverse effects*
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Diabetes Mellitus
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Fatal Outcome
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Humans
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Hypokalemia/chemically induced*
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Male
10.Research Advances in Postmortem Chemistry.
Shun-qi HAN ; Zhi-qiang QIN ; Kai-fei DENG ; Jian-hua ZHANG ; Ning-guo LIU ; Dong-hua ZOU ; Zheng-dong LI ; Yu SHAO ; Ping HUANG ; Yi-jiu CHEN
Journal of Forensic Medicine 2015;31(4):287-297
Postmortem chemistry is becoming more and more essential in routine forensic pathology and has made considerable progress over the past years. Biochemical analyses of vitreous humor, blood, urine and cerebrospinal fluid may provide important information in determining the cause of death or in elucidating forensic issues. Postmortem chemistry may be essential for the determination of cause of death when morphological methods (diabetes mellitus, alcoholic ketoacidosis and electrolytic disorders) cannot detect the pathophysiological changes involved in the death process. It can also provide many information in other forensic situations, including myocardial ischemia, sepsis, inflammation, infection, anaphylaxis and hormonal disturbances. The most recent relevant research advances on glucose metabolism, liver function, cardiac function, renal function, sepsis, inflammation, infection, anaphylaxis and hormonal aspect are hereby reviewed.
Anaphylaxis
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Autopsy/trends*
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Biomarkers/analysis*
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Body Fluids/chemistry*
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Death
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Diabetes Mellitus
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Forensic Pathology/methods*
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Humans
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Postmortem Changes
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Sepsis
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Vitreous Body