Objective To detect the changes of serum complement 3(C_3) and C_4 in neonates with hypoxic-ischemic encephalopathy(HIE) and its effects on non-specific immunological function.Methods Sixty-seven neonates with HIE and 30 normal full-term infants were recruited in the study.According to characteristic of immunological function and severity of HIE,the neonates were divided into 1-3 day group(n=32)and 4-6 day group(n=35).In each group,it was divided into mild group and moderate-severe group.The complement in serum was detected by radial immunodiffusion assay with Behring Nephelometer 100 Analyzer.Results In 1-3 day group,levels of C_3,C_4 in serum were lower than those in control group(P

0.05).In 4-6 day group:percentages of CD3+ and CD4+ cells and ratio of CD4+/CD8+ in neonates with HIE were all obviously lower than those in control group(P0.05).The level of percentages of CD3+ and CD4+ cells and ratio of CD4+/CD8+ in 9 subjects with moderate-severe HIE were remarkably higher during recovery stage than those during acute stage(P

Aged ; Coronary Artery Disease ; Female ; Heart Ventricles ; Humans ; Vascular Fistula

Objective To observe the therapeutic effect of Qiliqiangxin capsule combined on refractory heart failure.Methods 60 patients with refractory heart failure were randomly recruited into a control group and a therapeutic group. The control group received the conventional treatment, and the therapeutic group took Qiliqiangxin capsule on the base of the conventional treatment (3 times a day, 4 granules/each time) combined with 120 mg cAMP meglumine. Treatment in both group lasted 14 days. Results: After the treatment, the therapeutic group showed improvement in the symptoms of heart failure. Comparing with the control group, the difference was significant (P＜0.05). Conclusion The therapeutic effect of Qiliqiangxin capsule combined on refractory heart failure is satisfied and can improve the quality obviously.

Objective To improve hand hygiene compliance among healthcare workers (HCWs)in a stomatological hos-pital,and effectively reduce healthcare-associated infection(HAI)rate.Methods According to WHO hand hygiene guide-line and implementation scheme of hand hygiene,a systemic and step by step hand hygiene promotion plan was formulated, the plan was divided into five steps:full alert,baseline investigation,improvement,tracking,and retrospective analysis. Hand hygiene compliance among HCWs before and after the promotion of activities was analyzed and compared. Results Through hand hygiene promotion activities,the questionnaires showed that average score of knowledge about hand hygiene among HCWs enhanced from 58.33 to 77.40 (increased by 32.69%);the average hand hygiene compliance rate increased from 24.28%(59/243)to 49.81%(133/267),the difference was significant(χ2 =35.331,P =0.000);hand hy-giene status at five moments for hand hygiene improved markedly.Conclusion Hand hygiene promotion has a significant in-fluence in hand hygiene among HCWs in this stomatological hospital.

Adult ; Aged ; China ; epidemiology ; Chronic Disease ; Cooking ; Fatty Liver ; epidemiology ; Female ; Humans ; Hyperlipidemias ; epidemiology ; Male ; Mass Screening ; Middle Aged ; Neoplasms ; epidemiology ; Obesity ; epidemiology ; Occupational Diseases ; epidemiology ; Surveys and Questionnaires

Animals ; Arteritis ; complications ; immunology ; Coronary Artery Disease ; etiology ; pathology ; Coronary Vessels ; pathology ; Models, Animal ; Rabbits

Objective To investigate the clinical effects of the pulse indicator continuous cardiac output (PiCCO) in goal-optimized fluid management on patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with respiratory failure.Methods A total of 71 AECOPD patients with respiratory failure who were hospitalized in department of Critical Care Medicine in our hospital were selected from October 2010 to September 2013.The PiCCOtechnology was applied to monitor intrathoracic blood volume index (ITBI) and extravascular lung water index (ELWI).We compared the relationship of ELWI with ITBI and oxygenation index,and a simple correlation analysis was used for statistical analysis.Results A significantly negative correlation was found between ELWl and oxygenation index (r=-0.743,P＜0.01).ELWI =14 ml/kg was defined as the cutoff value for the subgroup analysis.A negative correlation still was found between ELWI and oxygenation index in the subgroup with ELWI ＜ 14 ml/kg,but it had no significant difference (r=-0.533,P=0.080),while a significantly negative correlation was found in the subgroup with ELWI≥14 ml/kg (r=-0.961,P＜0.01).There was no significant correlation between ELWI and ITBI (r=0.477,P=0.072).ITBI=1 000 ml/m2 was defined as the cutoff value for the subgroup analysis.There is no significant correlation between ELWI and ITBI in the subgroup with ITBI＜1 000 ml/m2(r=0.338,P=0.116),but in the subgroup with ITBI≥1 000 ml/m2,a significantly positive correlation between ELWI and ITBI was found (r=0.677,P＜ 0.01).Conclusions The results suggest that when ELWI is higher than 14 ml/kg,ELWI becomes an important influencing factor for oxygenation,which can be decreased to improve oxygenation in patients with AECOPD,and when ITBI≥ 1 000 ml/m2,ITBI can be reduced by decreasing ELWI.PiCCO has an important clinical significance on a goal-optimized fluid management in AECOPD patients with respiratory failure.

Animals ; Cardiovascular Diseases ; genetics ; pathology ; Cell Proliferation ; Genes ; Microfilament Proteins ; genetics ; Muscle Proteins ; genetics ; Muscle, Smooth, Vascular ; pathology ; Rats

Background Diabetic retinopathy (DR) is a common microvascular complications of the retina,retinal vascular smooth muscle cells of large conductance calcium-activated potassium channels (BK) is a major factor in regulating vasomotor and hemodynamic.Currently,functional changes of BK channel in retinal artery smooth muscle cells (RASMCs) and its role in DR were rarely reported.Objective This study was to investigate the early vascular damage mechanisms in DR by detecting the changes of BK channels current,calcium concentration and open probability (NP0) of BK channel with different calcium concentration in RASMCs of normal and diabetic rats.Method Fifty SPF SD 8-12 weeks old rats were randomly divided into normal control group and diabetic model group.Forty diabetic rats was intraperitoneally injected with 60 mg/kg streptozotocin to form type 1 diabetic model,10 rats (the normal control group) were injected sodium citrate solution with the same manner.Fluorescent probe was applied to detect calcium concentration in rat RASMCs;RASMCs were isolated by using enzyme digestion,and BK-channel electric currents and calcium concentrations in the RASMCs were measured by whole-cell patch clamp technique and fluorescence assay,respectively.The NP0 of BK channel was measured by single patch clamp technique.Results Diabetic models were successfully established in 36 rats with the success rate 90％.When stimulation voltage is greater than 60 mV,the current density of BK channel in RASMCs of diabetic model group decreased;when stimulating voltage was 100 mV,the BK channel currents of RASMCs in the normal control group and diabetic model group were (100±23) PA/PF and (50 ± 7) PA/PF,the difference was statistically significant (t =19.80,P ＜ 0.05).After adding specific BK channel blocker African scorpion toxin 100 nmol,the BK channel current in the normal control group significantly reduced,and that in the diabetes model group was not significantly changed;the calcium ion concentrations in RASMCs were (123±11)nmol/L and (255± 10)nmol/L in the normal control group and diabetic model group,the difference was statistically significant (t =32.50,P＜0.05).When stimulation voltage was 60 mV,with increasing calcium ion concentration,the NP0 of BK channel increased (F =15.28,P＜0.05).Conclusions The electric current and NP0 of BK-channel are obviously reduced and the calcium concentration is evidently elevated in RASMCs of diabetic rats,suggesting that the abnormal of BK-channel is probably one of the important causes of retinal artery abnormal contraction in diabetic rats.