Objective To investigate the protective effects of green tea polyphenols(GTPs)on dopamine neuron loss in substantia nigra concomitant with a depletion of dopamine in corpus striatum induced by the N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)in mice as a model of Parkinson disease.Methods Male C57BL/6 mice were intraperitoneally injected with saline + saline(group A),saline + GTPs(group B),saline+MPTP(group C)and GTP+MPTP(group D)at 2-hour intervals for a total of 3 doses for MPTP and 5 doses for GTPs(10 or 50 mg/kg delivered).The animals were sacrificed 7 d after the last injection.Levels of dopamine and its metabolites in the corpus striatum were measured with high-performance liquid chromatography with an electrochemical detector(HPLC-ECD).Results Level of dopamine and its metabolites in the corpus striatum in group C decreased significantly compared with group A or B;however,those in group D(both 10 and 50 mg/kg)prevented these effects.Conclusion GTPs can protect the dopamine neurons from loss in MPTP-induced mice.

With the rapid development of modern life sciences, tens of thousands of noncoding RNAs have been discovered, their biological roles have been revealed.Non-coding RNA, as a research hotspot in the field of molecular biology, has been shown to regulate the development and progression of tumors.This paper mainly describes the current research of several non-coding RNA(miRNA, lncRNA, circRNA, tRF)in the regulation of tumor and its application in the precision medicine era.

Objective: Investigate the curative effect of acupuncture on dysphasia in hypophrenia children. Methods: One hundred and sixty children with hypophrenia and dysphasia were randomly divided into an acupuncture group of 80 cases, a language training group of 40 cases and a composite group of 40 cases for clinical observation. Total intelligence quotient (TIQ), vocal intelligence quotient (VIQ) and practical intelligence quotient (PIQ) were compared between pretreatment and posttreatment in the children themselves and between the groups before and after treatment. Results: The total efficacy rate was 77.5% in the acupuncture group, 82.5% in the composite group and 52.5% in the language training group. Conclusion: Acupuncture significantly raises TIQ, VIQ and PIQ in the sick children. Language training only raises VIQ to some degree.Acupuncture in cooperation with language training is an effective way to treat this disease.

0.05). After lamivudine treatment, the rate of the transform negative of HBV DNA in genotype C was significantly higher than that in genotype B, and the rate of the bounce of HBV DNA in genotype B was higher than that in genotype C (P 0.05). CONCLUSION: There may be a relation between the effect of lamivudine and the genotype of Hepatitis B virus. The effect of the lamivudine treatment may be better in the patients with genotype C.

Myopia is not only a global public health problem, but also a significant socio-economic problem. There are various hypotheses about the pathogenesis of myopia, which is basically the result of the combination of environmental and genetic factors. Although a large number of epidemiological studies have been carried out on the influencing factors of myopia, most of them are cross-sectional studies, longitudinal cohort studies are relatively few. This paper will summarize the influencing factors of myopia at homeland and abroad in recent years.

BACKGROUND: As magnetic resonance (MR) contrast, a large number of clinical and experimental researches have been done on superparamagnetic iron oxide (SPIO), while the report on the labeled cell passaged cells is rare.OBJECTIVE: MR imaging was performed to the labeled bone mesenchymal stem cells (BMSCs) and its passaged cells in vitro, in order to establish the base of monitoring magnetic labeled BMSCs with magnetic resonance imaging (MRI) in vivo.DESIGN, TIME AND SETTING: The cytological in vitro experiment was performed at the Imaging Research Institute and Institute of Rheumatology and Immunology of North Sichuan Medical College Hospital from June 2006 to January 2007. MATERIALS: Two clean female albino rats (Animal Center, North Sichuan Medical College), SPIO (Schering AG,Germany) were used in this study.METHODES: Bilateral femur and tibia bone marrow was extracted from rats. BMSCs were harvested and purified using the adherent method, and then labeled with 600 μL ferric oxide-polylysine compound (42 mg/L iron concentration) in vitro. MAIN OUTCOME MEASURES: Cell maker-positive rate and the MR signal intensity were respectively measured to the labeled cells and its passaged cells under the inverted microscope and magnetic resonance imaging. RESULTS: Following Prussian blue staining, labeling rate of SPIO labeled cells at the first passage was 100%. With increased passage, the labeling rate was reduced from the first to fifth passages. Compared with non-labeled PBS control, there was no significant difference in signal intensity in the first and second passages cells, but the signal intensity percentage was gradually decreased with signal intensity of increased cell passage from the third passage. Cell labeling rate was negatively correlated with T2~*WI signal intensity (r=-0.986 6, P <0.005). CONCLUSION: The iron particles in the magnetic labeled cells can be passaged to the offspring cells, and can be monitored in a certain period of time with MRI in vitro. These results firstly introduced that SPIO-labeled cell iron particles can decrease with cell passage.

Objective To explore the optimal situation of labeling bone mesenchymal stem cells (BMSCs) with superparamagnetic iron oxides (SPIO) mediated by poly-L-lysine (PLL), and determine the most optimal protocol of magnetic resonance imaging according to the patterns of MR in vitro. Methods BMSCs were isolated from white rat and purified, incubated with SPIO-PLL complexes at the range of concentrations (0, 4.2, 8.4, 21, 42, 84 ?g Fe per ml medium). The labeling ratio and distribution of SPIO particles in BMSCs, and the morphological evidence of abnormal visualization were evaluated by Prussian blue staining, fluorescent microscope and electron microscopy. MTT growth curves and magnetic resonance imagings were obtained at the range of concentrations. Trypan blue exclusion test was performed to elevate the viability of BMSCs labeled with PLL at the range of concentrations (0, 0.05, 0.25, 0.5, 1.0, 5.0 ?g PLL per ml medium). Results The cellular labeling ratio was strongly correlated to the concentrations of SPIO (P

Background As a new tonometer,it is necessary to assess the clinical value of Icare rebound tonometer.Objective This study was to compare the intraocular pressure(IOP)values measured by Icare with that measured by GAT,and discuss the clinical value of leare rebound tonometer. Methods IOP measurement was performed on 152 eyes of 78 subjects with suspicious glaucoma,glaucoma,refractive error and normal examinnee by Icare and GAT respectively.The Icare IOP was measured firstly and then the GAT IOP was carried out with the 3-or 5-minute interval.The IOP values were compared between Ieare and GAT.This study was approved by Ethic Committee of Wuhan General Hospital of Chinese PLA.Written informed consent was obtained from each subject prior to this study. Results The mean IOP values of Icare and GAT were(19.16±5.03)mmHg and(18.41±4.52)mmHg respectively.The differences between Icare IOP and CAT IOP were less than or equal to 1 mmHg in 96 of 105 eyes(63.2%).The positive correlation was found between the Icare IOP and GAT IOP(r=0.940,P<0.01).The Ieare IOP was lower than that of GAT when IOPIcare<16 mmHg,however,the IOP of Icare were higher when IOPIcare≥6 mmHg;the IOP of Icare were higher than that of GAT in the total CCT range.The correlation coefficients of IOP of Icare or CAT with CCT were 0.341(P<0.01)and 0.333(P<0.01),respectively. Conclusion Compared with GAT,Icare is more feasible in clinic because it is practicable and reliable.

Objective To observe the anatomic relationship between the lateral sural artery perforators and the nutrient vessels of the lateral sural cutaneous nerve that were combined to design a compound muscle flap.And to observe the effect of this flap clinical application.Methods Ten adult and fresh cadaver lower limbs were observed the anatomic relationship between the lateral sural artery perforators and the nutrient vessels of the lateral sural cutaneous nerve,and were combined to design a compound muscle flap.Clinically applied in 5 cases,and the flap sizes ranged from 6 cm × 9 cm to 9 cm × 12 cm.Results After into the muscle,the lateral sural artery issued 3-5 perforating branches (OD:0.2-1.8 mm) along the way,which provided nutrition for the lateral head of the gastrocnemius and the surrounding skin and involved in the formation of shallow and deep fascia vascular network with the nutrient vessels of the lateral sural cutaneous nerve.From Feburary 2009 to November 2011,the compound pedicled muscle flap were clinically applied in 5 cases to repair the skin defects of the anterior knee and proximal tibia.Flaps in 4 cases completely survivedand 1 case had partial superficial necrosis on the distal part of the flap,which healed after dressing change.Conclusion The compound pedicled muscle flap has two sets of blood supply that could expand the area of the flap,which could guarantee its feeling.This kind of flap could repair the complicated skin and soft tissue defects around the knee joint.

Objectives To observe the effects of electroacupuncture (EA) treatment of different intensities on learning-memory function and expression of β-amyloid protein 1-40 (Aβ1-40) in hippocampus CA1 region in rats with vascular dementia (VD) and to seek the best intensity of EA treatment.Methods A total of 60 male SD rats (SPF grade) were enrolled.Eight rats were selected as sham operation group with random number table method;others were used to copy the VD model with the modified four-vessel occlusion (4-VO) method.According to the random number table method,the successful model of the rats (n=24) were completely randomly divided into a model group,a 1 am EA group (frequency,2/15 Hz,intensity,1 mA,needle retention time,20 min),and a 3 mA EA group (frequency,2/15 Hz,intensity,3 mA,needle retention time,20 min;n=8 in each group).DU20 (BaiHui) and DU14 (DaZhui) in the EA group were acupunctured once a day for 10 d,and took a rest for 2 d as 1 treatment course.After 2 treatment courses,Morris water maze test was used to detect the ability of learning and memory of rats in each group.Fluorescent quantitative polymerase chain reaction was used to detect the expression level of Aβ1-40 mRNA.Results The mean escape latencies of the water maze test from 2 to 5 days in the sham operation group,model group,1 mA EA group,and 3 mA EA group were 46.8±1.9,40.6±2.3,24.6±1.5,19.4 ±1.2 s;56.3±3.5,51.2±2.6,45.9±2.1,40.8±1.4 s,52.7±1.5,46.0±2.3,31.3±1.2,27.7±1.6 s;and 50.8±3.9,41.5±2.1,29.0±1.1,25.6±1.3 s,respectively;the first time across the original platform were 23.3±1.6,53.9±1.3,30.2±1.4,and 28.1±0.8 s,respectively;the first across the original platform within 120 s were 9.4±0.9,2.6±0.5,6.4±0.7,and 7.2±0.9,respectively;the expression levels of Aβ 1-40 mRNAs in the CA1 regions were 17.3±1.1,40.7±1.1,24.0±1.7,and 22.4±1.8,respectively.There were significant differences among the groups (the F values were 195.88,861.605,103.876,and 380.609,respectively;all P<0.01).Compared with the model group,the mean escape latencies,the first time across the original platform were reduced significantly.Compared with the model group,the times across the original platform within 120 s were increased significantly.Compared with the model group,the gene expression level of Aβ 1-40 mRNA in the center of CA1 region was decreased significantly,and the 3 mA EA group was significantly superior to that of the 1 mA EA group.The difference was statistically significant (all P<0.05).Conclusion EA may improve the learning and memory ability in VD rats and lower the expression level of Aβ 1-40 mRNA in the CA1 region of hippocampus.Effect of 3 mA EA is better than that of the 1 mA EA.