1.Progress Toward Molecular Determinants of the Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae
Guozhong TIAN ; Li ZHANG ; Zhujun SHAO
Chinese Journal of Vaccines and Immunization 2008;0(02):-
Haemophilus influenzae (Hi) is a pathogen exclusively found in humans. It causes a wide range of infections from the upper respiratory tract to serious invasive diseases. Such as pneumonia, septicemia and meningitis. Strains of Hi are usually classif ied into six serotypes a to f and nontypeable H. influenzae (NTHI) according to the antigenicities and compositions of their polysaccharide capsules. Hib was a common cause of serious infections in younger children. The polysaccharide-protein conjugate vaccines against Hib had almost eliminated H. influenzae as a cause of pediatric meningitis. However, NTHI remains an important pathogen, particularly in children and the elderly. Efforts to understand and control NTHI disease have been hampered by the diversity of these bacteria. This review introduced the study progress about pathogenic mechanism of NTHI. In order to provide the help for development of vaccine, clinic treatment and prevent the occurrence of diseases causing by NTHI.
2.Identification of Seven Species of Clematis(Clematis L.)by RAPD Analysis
Rong ZHANG ; Jienben SHAO ; Xueming TIAN
Chinese Traditional and Herbal Drugs 1994;0(11):-
Identification of seven species of Clematis L. (C. chrysocoma Fr.,C. amendii Fr.,C. fasciculiolia Fr.,C. ranunculoides Fr.,C. rubifolia C. H. Wright,C. florida Thunb. and C. brevicaudata DC. )was studied by RAPD analysis. Results showed that the differences of RAPD between different species were significant,but nonsignificant between same species obtained from different localities
3.Acupuncture at Yinlingquan (SP 9), Taichong (LR 3) mainly plus blood-letting therapy for 52 cases of gout.
Hong-Yan SHAO ; Tian-You HE ; Li ZHANG
Chinese Acupuncture & Moxibustion 2013;33(6):526-526
Acupuncture Therapy
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Adult
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Aged
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Bloodletting
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
4.Long snake moxibustion for 38 cases of gout.
Tian-You HE ; Li ZHANG ; Hong-Yan SHAO
Chinese Acupuncture & Moxibustion 2014;34(6):605-606
Acupuncture Points
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Adult
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Aged
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
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Moxibustion
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Treatment Outcome
5.Anesthesia for aortic valve insufficiency complicated with extremely dilated left ventvicle
Yulin TIAN ; Xiushu LUAN ; Yanbin SHAO ; Huixian LI ; Dongya ZHANG
International Journal of Surgery 2010;37(6):388-390
Objective To retrospectively review the experience of anesthesia for aortic valve insufficiency complicated with extremely dilated left ventricle.Methods The patients were premedieated with morphine 10 mg and scopolamine 0.3 mg IM.Anesthesia was induced with etomidate 0.1-0.2 ms/ks.sufentanil 1μ/kg and pipecuronium 0.1 mg/kg.After the patients were intubated,anesthesia wag maintained beween 1.0%and 1.5% isoflurane and intermittent Ⅳ boluses of sufentanil and pipecuronium.ECG,direct BP,SpO2,PET CO2,CVP and body temperature were monitored during anesthesia.Result All patients Were rehabilitated without ventricular fibrillation and arrhythmia.Conlusions Measures are taken before the surgery to maintain preload,increase heart rate,reduce afterload moderately and maintain myocardial contractility.After the surgery,maintenance of preload is helpful for ensuring adequate forward flow and benefcial to patients using dopamine and nitrolycerin.
6.A study of the expression of ANGPT1,ANGPT2 and VEGF in mouse model of acute myeloid Leukemia
Hong YANG ; Fengying TIAN ; Zeping ZHOU ; Liang SHAO ; You ZHANG
Chongqing Medicine 2015;(5):586-589
Objective To construct mouse model of acute myeloid leukemia and detect the expression of ANGPT 1 ,ANGPT2 and VEGF gene on the cells its as well as the clinical significance .Methods The HL‐60 cells were transfected to NOD/SCID mouse through abdominal injection to construct mouse model of acute myeloid leukemia .Then identify mouse model by histopathology and Flow Cytometry .The expression of ANGPT2 ,ANGPT1 and VEGF mRNA in the tumor tissues of mouse model was detected by real‐time fluorescence quantitative PCR .The expression of ANGPT2 will be analyzed on the survival time of mouse model by Spearman′s correlation method .Results Mouse model has been successfully identified by histopathology and Flow Cytometry .The expression of ANGPT2 and VEGF in mouse mode was significantly detected ,which was that of higher than normal group (P<0 .05) .The expression of ANGPT1 was lower than that of ANGPT2 and VEGF ,there was no significant difference between AN‐GPT1 and normal groups (P>0 .05) .The higher expression of ANGPT2 in mouse model had a short survival time in mouse with acute myeloid leukemia .Conclusion This study showed that ANGPT2 mRNA was over‐expressed in acute myeloid leukemia .The increasing expression of ANGPT2 mRNA may lead to poor prognosis in mouse with acute myeloid leukemia .
7.Perioperative management of nasal septum perforation.
Tian-zhen ZHANG ; Shao-hong JIANG ; Qiang WANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(11):943-945
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Nasal Septal Perforation
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surgery
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Perioperative Care
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Young Adult
9.Application of 99mTc-DTPA renography in the determination of GFR in living kidney donors
Xiuyi ZHAO ; Yahui SHAO ; Jun TIAN ; Ben SUN ; Xiangtie LI ; Aimin ZHANG ; Junwen HAO ; Chuanfu LI
Chinese Journal of Organ Transplantation 2010;31(8):481-484
Objective To investigate the clinical application of 99mTc-DTPA renography in evaluating the glomerular filtration rate (GFR) in living donor kidney transplantation and to assess the dependence of GFR on age and gender in living kidney donors. Methods There were 212 consecutive potential donors in the study. The potential donor evaluation process included as follows: general health status, liver and kidney ultrasound, hepatitis virus infection and HLA-DR matching. If the results met the general requirements for the donor selection criteria, the GFR was measured using the 99mTc-IDTPA renography according to standard procedure (gates method). The GFR ≥ 1.33 ml/s was considered normal, < 1.17 ml/s was defined as the lower limit for donor GFR, and 1.17 ml/s ≤GFR < 1.33 ml/s further underwent measurement of creatinine clearance (CCr). If the CCr was normal, the GFR was considered normal, and otherwise, potential donors gave up kidney donation.All the donors meeting the donor selection criteria were divided into four age groups. On the other hand, the total donors were divided into the groups aged > 55 years and aged ≤ 55 years. The impact of gender and age on GFR was evaluated preoperation due to age-related changes and gender using Kendall's tau-b correlation coefficient. Results In 212 potential donors, 137 cases had a GFR ≥ 1.33ml/s, 55 cases 1.17 ml/s ≤ GFR < 1.33 ml/s and 20 cases GFR < 1.17 ml/s. Thirty-one cases of potential donors with 1.17 ml/s ≤ GFR < 1.33 ml/s gave up kidney donation due to abnormal CCr or other security considerations. 161 (56 females, 105 males) were qualified as successful donors, and the donor age was 42. 91 ± 11.90 years (range 20 to 62 years). The preoperative total GFR (ml/s) in living kidney donors was calculated as 1.51 ± 0.22 for males, it was 1.45 ± 0.18 for females respectively (P>0.05). Among the four age groups, there was no significant difference in GFR (P>0.05). The GFR in the donors aged > 55 years and aged ≤ 55 years was 1.48 ± 0.22 and 1.49 ±0.17 respectively (P>0.05). Correlation analysis revealed that the GFR in all the donors was not related with age (r = -0. 033, P = 0. 69). Also, there was no correlation between age and GFR in men and women(r= -0.053, P=0.571; r= -0.019, P=0.754). Conclusion 99mTc-DTPA renography is reliable and reproducible for the determination of GFR in living kidney donors. In view of acute donor shortage and if properly screened, kidneys with 1.17 ml/s≤ GFR < 1.33 ml/s can be used without increasing the risk to donor. The GFR is not correlated with the age and gender.
10.Clinical analysis of pulmonary alveolar proteinosis and cases with combination of severe infection
Hong ZHANG ; Chi SHAO ; Min PENG ; Xinlun TIAN ; Wenbing XU ; Boqiang CA
Chinese Journal of Postgraduates of Medicine 2009;32(10):24-26
Objective To describe and analyze the misdiagnosis and mistreatment status of pulmonary alveolar proteinosis (PAP), especially cases with combination of severe infection. Method The misdiagnosis and mistreatment of 84 PAP patients and 6 cases with combination of severe infection before admitting to PUMCH was analyzed retrospectively. Results 66.7%(56/84) had experienced misdiagnosis before admission. 32.1% (27/84) were misdiagnosed with idiopathic interstitial pneumonia (IIP) and 14.3% (12/84) with pulmonary tuberculosis. 88.1%(74/84) had received anti-infection treatment, 33.3%(28/84) received eortieosteroids, and 19.0%(16/84) received anti-tuberculosis treatment. The percentage of the pa-tients who had taken the bronchoscopic examination outside the PUMCH was 53.6%(45/84). The PAP pa-tients diagnosis was established through bronchoscopic examination (including BAL and TBLB) in 86.9% (73/84) patients. There were 6 patients who had severe infection when PAP established. All of these 6 cases had been misdiagnosed with IIP and received corticosteroids treatment, 2 of whom died. Conclusions The misdiagnosis and mistreatment happens commonly in PAP patients. The misuse of corticosteroids makes some PAP cases get the severe infection. The image feature and the examination of bronchoscopy, BAL and TBLB are the key point in avoiding misdiagnosis. The administration of corticosteroids in uncertain patients should be avoided.