OBJECTIVETo study the biological behavior of cardial cancer and its influence on surgical management.

METHODSComplete clinicopathologic data of 46 cases with cardial cancer undergoing radical gastrectomy was investigated retrospectively. The relationships between tumor Borrmann type, depth of invasion, growth pattern, lymph node metastasis and 5-year survival rate postoperative were analyzed.

RESULTSOf 46 cases, Borrmann type III, Type IV and Type II was 76% (35/46), 18% (8/46) and 6% (3/46) respectively; 5-year survival rate was 40% (14/35), 0 (0/8) and 100% (3/3) respectively. In respect of the depth of invasion, pT(2) was 31% (14/46) cases with 71% (10/14) lymph node metastasis; and 5-year survival rate was 64% (9/14). pT(3) was 15% (7/46) cases with 86% (6/7) lymph node metastasis; and 5-year survival rate was 57% (4/7). And pT(4) was 54% (25/46) cases with 92% (23/25) lymph node metastasis; and 5-year survival rate was 12% (3/25). The growth pattern in 87% (40/46) cases was infiltrative; and 5-year survival rate was 28% (11/40); the growth pattern in 13% (6/46) cases was expansive; and 5-year survival rate was 100% (6/6).

CONCLUSIOND(2)(+) radical total gastrectomy should be performed on the developed cardial cancer, and if necessary, resection of body and tail of pancreas should be chosen.

Aged ; Aged, 80 and over ; Cardia ; Female ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Prognosis ; Stomach Neoplasms ; pathology ; surgery

Objective To investigate the association of first permanent molars caries prevalence with primary molar caries experience.Methods Cluster,stratified and random sampling method,was used and 3680 children 6-to 10-year-old were randomizly selected from primary schools of Jinan city.Caries status of first permanent molars,decayed-missed-filled surfaces (DMFS,dmfs),decayed-missed-filled teeth (DMFT,dmft),were examined.Chi-square test was used to analyze the relationship among the caries incidence and the age,sex and to analyze the relationship between the first permanent molars DMFT,DMFS and the primary molars dmft,dmfs.Results Twenty point seven percent(763/3680) of children had first permanent molars caries,significantly higher(P ＜ 0.01) in girls [28.9％ (509/1762)] than in boys [13.2％ (254/1918)],a positive correlation (r =0.31,P ＜ 0.01) was found between caries experiences of primary molars (dmft) and caries of first permanent molars (DMFT).Higher permanent molar caries was found in subjects with dmft higher than 3 or dmfs higher than 19.Conclusions Sex,age and the caries experience of primary molars were factors closely related with the caries rate of the first permanent molars.Enhanced prevention is suggested on the first permanent molars of children who has higher prevalence of caries in primary molars.

AIMTo evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects.

METHODSA cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP, respectively. The mixed cDNA probes were then hybridized with cDNA microarray (each containing 4096 unique human cDNA sequences). The fluorescent signals were scanned and the values of Cy5-dUTP and Cy3-dUTP on each spot were analyzed and calculated. In situ hybridization was employed to detect the expression of Rap1A in the testes of 10 fertile and 39 azoospermic subjects.

RESULTSOne hundred and twenty-eight differentially expressed genes were found to be possibly related to azoospermia, of which 56 were up-regulated and 72, down-regulated genes. The mRNA expression of Rap1A in the spermatogenic cells of azoospermic was stronger than that in those of the fertile testes.

CONCLUSIONRap1A may play certain roles in the development of azoospermia.

Adult ; Gene Expression ; Humans ; In Situ Hybridization ; Male ; Oligospermia ; metabolism ; RNA, Messenger ; analysis ; Spermatozoa ; chemistry ; Testis ; chemistry ; rap1 GTP-Binding Proteins ; genetics ; physiology

OBJECTIVETo investigate the contamination state of Legionella in cooling water samples from different places in Wuxi city and reveal the molecular biological characteristics of Legionella strains.

METHODS112 parallel water samples (500 ml each) were collected from 56 sites in Wuxi city during year 2009 - 2010. The samples were used for Legionella test and quantitative culture. The isolated Legionella strains were used for serotyping, pulsed-field gel electrophoresis (PFGE), sequence-based typing (SBT), and intracellular growth were tested.

RESULTSThe positive proportion of Legionella was 39. 3% (22/56) among all sampling sites. A total of 29 Legionella strains were isolated, and the serotypes include LP1, LP3, LP5 and LP6. LP1 serotype was the major one with a proportion of 65.5% (19/29). 29 Legionella strains got 17 PFGE types. There were 10 SBT types among 10 Legionella strains with different PFGE types. Comparing to LP1 strain (ATCC 33152), WX2011062 (LP6) and WX2011067 (LP5) had strong intracellular growth ability in mouse peritoneal macrophages J774 cell line (the amount of intracellular bacteria on day 0 after infection were (5.5 +/- 1.32) x 10(5), (3.9 +/- 0.60) x 10(5), (7.8 +/- 0.76) x 10(5) CFU/ml, respectively; the amount of intracellular bacteria on day 3 after infection were (58.3 +/- 1.61) x 10(5), (2700.0 +/- 655.74) x 10(5), (3066.7 +/- 208.17) x 10(5) CFU/ml, respectively).

CONCLUSIONThe Legionella contamination existed in cooling water samples from different places in Wuxi city. Legionella strains isolated showed high genetic variation. Some Legionella strains had vigorous intracellular growth ability.

Air Conditioning ; Animals ; Cells, Cultured ; Environmental Microbiology ; Legionella ; genetics ; growth & development ; isolation & purification ; Legionella pneumophila ; growth & development ; isolation & purification ; Macrophages ; microbiology ; Mice ; Serotyping ; Water Microbiology

OBJECTIVETo evaluate the serum concentrations of E- selectin, integrinbeta(1) subunit and intercellular adhesion molecule-1 in gastric cancer patients and their clinicopathological significance.

METHODSThe serum levels of adhesion molecules E- selectin,intercellular adhesion molecule- 1 (ICAM- 1), and integrinbeta(1) were measured by enzyme-linked immunosorbent assay (ELISA) in 47 health subjects (control group) and in 57 patients with gastric cancer (gastric cancer group) before operation and 7 days after operation. Serum levels of above three factors were compared between the two groups.

RESULTSThe serum concentrations of E- selectin, integrinbeta(1) subunit and ICAM- 1 were higher in gastric cancer group with positive rate of 24.6% ,33.3% ,28.1% respectively. ICAM- 1 and integrinbeta(1) were significant higher in gastric cancer group than that in the control group (P< 0.01),but there was no significant difference in E- selectin between two groups (P=0.64). Serum concentrations of E-selectin, ICAM-1,and integrinbeta(1) were significantly correlated with clinicopathological features as following: clinicopathological stage,invasion depth,lymph node involvement,and presence of distant metastases(P< 0.05,P< 0.01). The serum levels of E- selectin, ICAM- 1, and integrinbeta(1) were decreased significantly after radical resection of gastric cancer,but not in patients with unresectable tumor. Elevated levels of three molecules were significant prognostic factors for patients with gastric cancer,but it could not independently be used to evaluate tumor stage.

CONCLUSIONSSerum concentrations of E- selectin, ICAM- 1,and integrinbeta(1) may reflect tumor progression and metastasis.

Adult ; Aged ; Biomarkers, Tumor ; blood ; E-Selectin ; blood ; Female ; Humans ; Integrin beta1 ; blood ; Intercellular Adhesion Molecule-1 ; blood ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Serum ; metabolism ; Stomach Neoplasms ; metabolism ; pathology

Homo sapiens longevity assurance homologue 2 (LASS2) is a novel gene isolated from a human liver cDNA library by our laboratory, and it is a human homologue of the yeast longevity assurance gene LAG1 (Saccharomyces cerevisiae longevity assurance gene). According to our previous results, LASS2 could interact with subunit c of vacuolar type H(+)-ATPase (V-ATPase), and the overexpression of LASS2 could inhibit the cell growth of a human hepatocellular carcinoma (HCC) cell line, SMMC-7721. In order to understand the role of the interaction between LASS2 and V-ATPase in HCC cell growth, we transiently transfected plasmid pCMV-HA2-LASS2 into HCCLM3, a HCC cell line without the significant expression of endogenous LASS2. The pH-sensitive fluorescence probes, BCECF and BCECF-AM, were used to measure the intracellular and extracellular H(+) concentrations of HCCLM3 cells respectively. The effect of LASS2 gene on apoptosis was evaluated with Annexin-V/FITC and propidium iodide (PI) by flow cytometry. Western blot was used to detect cytochrome c (Cyt c) in the cytosol and mitochondria, as well as pro-caspase-3 in cytosol. The results showed that the cell growth of LASS2-transfected HCCLM3 cells was significantly inhibited compared with that of the mock control. LASS2 transfection increased intracellular H(+) concentration of HCCLM3 cells, while decreased extracellular H(+) concentration. Moreover, LASS2 transfection significantly enhanced the apoptosis of HCCLM3 cells. In LASS2-transfected cells, the amounts of Cyt c increased in the cytosol, while decreased in the mitochondria. Meanwhile, the expression of pro-caspase-3 in the cytosolic extracts was decreased. These results implicate that LASS2 gene might increase intracellular H(+) of HCC cells via the interaction with V-ATPase, thereby inducing cell apoptosis through mitochondrial pathway.
Apoptosis ; Carcinoma, Hepatocellular ; pathology ; Caspase 3 ; metabolism ; Cell Line, Tumor ; Cell Proliferation ; Humans ; Liver Neoplasms ; pathology ; Membrane Proteins ; metabolism ; RNA, Small Interfering ; Sphingosine N-Acyltransferase ; metabolism ; Transfection ; Tumor Suppressor Proteins ; metabolism ; Vacuolar Proton-Translocating ATPases ; metabolism

Abstract: Objective　To construct SARS-CoV-2 receptor binding domain molecular probe for monoclonal memory B cell sorting and obtain RBD specific neutralizing antibodies from peripheral blood mononuclear cells (PBMCs) of COVID-19 convalescents by single-cell sorting. Methods　The SARS-CoV-2 RBD sequence was downloaded from GenBank, and the Avi tag and 6-histidine tags were added at the C-terminal. After codon optimization, it was chemically synthesized, cloned into the pDRVI1.0 vector, expressed after transfection of 293F cells, and biotinylated consequently. RBD-specific B cells were sorted out with this probe1 from the PBMCs of convalescents recovered from COVID-19. After B cells were lysed, the variable regions of heavy chain and light chain were amplified, cloned into the antibody expression vector, and transfected into 293F cells to express the antibody. Then the antibody was purified from the supernatant using protein A column and SARS-CoV-2 pseudovirus was used to test their neutralizing activity. Results　RBD-Avi probe was produced and successfully biotinylated sequentially with an efficiency of 30%-50%. Western blot analysis revealed that the biotinylated probe was recognized by the antibodies purified from COVID-19 convalescent plasma. Using this probe, 7 and 16 RBD-specific memory B cells were successfully isolated from the PBMCs of two convalescent individuals, accounting for 0.24% and 0.17% of the total cell population, respectively. After amplifying the variable regions of antibody heavy and light chains from the lysed B cells, 7 and 12 pairs of antibody heavy-light chains were obtained. A total of 16 antibodies were expressed in the convalescent individuals, and most of the purified antibodies showed neutralizing activity against the pseudovirus, with IC50 values of 6 antibodies below 1 μg/mL. The IC50 values of XJ-A9 and SCF-F1 against the wild-type pseudovirus were 0.07 μg/mL and 0.35 μg/mL, respectively. Conclusion　The SARS-CoV-2 RBD molecular probe constructed in this study has good antigenicity, and the isolated antibodies present neutralizing activity against wild-type SARS-CoV-2 pseudovirus.

Objective To understand lifestyle and self-management ability of high risk population of cardiovascular and cerebrovascular disease,and explore the effect of health education.Methods 2 400 high-risk in cardiovascular and cerebrovascular disease,35-85 years old,were selected and underway health intervention.Lifestyle and self-management ability were surveyed by questionnaire four times in two years.The heath status was compared.Results Self management ability was different in different populations: female(1 003 cases)was significantly higher in males(850 cases); higher degree of education ranked above the lower education level;higher income ranked above the lower income persons.The Chi square values were 134.69,155.86,85.90,respectively and the all P values were less than 0.05.Self-management ability was positively correlated with the number of visits(x2 =69.55,P ＜ 0.05).Fasting blood glucose level was(5.32 ± 0.63)mmol/L,2 h blood glucose after meal was(6.35 ±2.48)mmol/L,HbA1c was(4.8 ± 1.4)％,TC was(3.2 ± 1.4)mmol/L,TG was(1.5 ±0.7)mmol/L,and LDL-C was(1.7 ±0.6)mmol/L,respectively after intervention,and those index before intervention were(7.24±2.18)mmol/L,(11.17 ±3.44)mmol/L,(9.1 ±3.4)％,(4.1 ±1.2)mmol/L,(4.1 ± 1.2)mmol/L,(3.1 ± 1.2)mmol/L,respectively,and the differences were statistically significant(F=1.535,2.124,1.191,0.298,5.073,1.282; P＜0.05).Conclusions Population at high risk of cardiovascular and cerebrovascular diseases has low self-management ability.Reinforcement of health intervention could improve their self-management ability and promote their health status.

Objective To explore the application of nursing performance distribution system in high quality of nursing care so as to improve the job enthusiasm of nursing staff and improve the quality of nursing work in hospital.Methods Nursing performance distribution system was implemented in the high quality nursing units,then,the satisfaction of patients and doctors on the nursing care,the quality of nursing,nursing errors occurrence were compared before after the nursing performance distribution system was implemented.Results After the implementation of performance appraisal system,the quality of nursing work was significantly improved,that compared with the performance distribution system not implemented [(96.38 ± 0.91 ) vs (92.36 ±0.72),(98.26 ±0.90) vs (90.38 ±0.75),(97.39 ±0.93) vs (89.26 ±0.76),(98.69 ±0.96)vs (91.57±0.78),(97.28±0.74) vs (89.93 ±0.76),(98.57 ±0.98) vs (91.23 ±0.78),(99.26 ±0.93)vs (91.38 ± 0.79 );t =36.647,90.990,82.833,10.910,636.529,60.461,13.177,respectively;P ＜ 0.05 ) ].And the satisfaction of patients and doctors on nursing care both was significantly improved ( P ＜ 0.05 ),nursing errors occurrence was significantly reduced after the performance appraisal system implemented ( x2 =0.845,P ＜ 0.05 ).Conclusions Nursing performance distribution system implementing into the quality of nursing care management reflect the principle of good payment for good work,payment according to work,and it is a scientific and efficient management mode for nursing management.Performance assossment can ensure the stability and continuity of quality improvement.

Objective To study the protection of total Hedysaripolysaccharide in lipopolysaccharide (LPS) induced acute lung injury (ALI) of mice.Methods The mice were randomly divided into 6 groups:normal group,model group (8 mg · kg-1 lipopolysaccharide),control group (dexamethasone 3 mg· kg-1),and experimental groups in low,middle and high dose (50,100,200 mg · kg-1 total Hedysaripolysaccharide).Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10)levels in serum were measured with enzyme-linked immunosorbent assay.Total antioxidant capacity (T-AOC),the activities of super oxide dismutase (SOD) and malondialdehyde (MDA) contents in serum were detected by spectrophotometer.And DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis.Results The serum TNF-α and IL-10 levels in model group were (156.23 ± 7.39),(44.24 ± 2.89) ng · L-1,which in middle and high dose of experimental groups were (139.45 ± 6.57),(35.30 ± 1.88);(120.13 ± 6.81),(33.11 ±2.71) ng · L-1,the difference was significant(all P ＜0.01).T-AOC,SOD activities in middle and high dose of experimental groups were (5.91 ± 0.10),(70.00 ± 3.04);(6.10 ± 0.09),(76.21 ± 4.03) U · mg-1,which were obviously higher than those of model group which were (5.56 ± 0.13),(67.68 ± 4.86) U · mg-1 (all P ＜0.01).MDA contents of middle and high dose experimental groups were (5.01 ± 0.11),(4.17 ± 0.12) nmol · mg-1,which were obviously lower than that of model group with(5.55 ±0.11) nmol · mg-1 (all P ＜0.01).DNA tail length (TL) were (5.81 ±0.10),(5.24 ±0.10) μm,tail moment (TM)were (2.86 ±0.12),(2.71 ±0.16) μm,and Olive tail moment(OTM) were (2.67 ±0.10),(2.17 ±0.18) μm in middle and high dose of experimental groups,which were significantly lower than those of model group:TL was (6.45 ± 0.08) μm,TM was (3.22 ± 0.14) μm,and OTM was (3.35 ± 0.12) μm,the differences were all significant (all P ＜ 0.01).Conclusion Total Hedysaripolysaccharide had a protective effect against the lipopolysaccharide-induced acute lung injury.The possible mechanisms may be due to the regulation the proportional imbalance of TNF-α and IL-10,as well as the balance oxidative and anti-oxidative.