1.Effect of Musicotherapy on Agitation Behaviors in Patients with Aged Dementia
Yu-mei LI ; Lin-ping CHENG ; Shao-kuan HUANG ; Yongping DENG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(10):901-903
ObjectiveTo explore the effect of musicotherapy on agitation behaviors in patients with aged dementia (AD).Methods22 AD patients were treated with different musicotherapies, and examined with Mini-Mental State Examination (MMSE), Cohen-Mansfield Agitation Inventory (CMAI) and self made sociality psychological inventory before and after treatment.ResultsAfter musicotherapy, the incidence rate of agitation behaviors of AD patients decreased significantly ( P<0.001). The incidence rates of agitation behaviors among patients with low, middle and high MMSE scores were significantly different ( P<0.001). After musicotherapy, both depressions of patients' cognitive function and scores of agitation behaviors were significant different compared with that before treatment (P<0.01). Effectual rates of musicotherapy on sociality psychological emotion, communication, language and intercourse of this group patients were 86.36%, 90.91%, 72.73% and 77.27%, respectively.ConclusionMusicotherapy can effectively alleviate the symptoms of agitation behaviors in AD patients.
2.Preparation of monoclonal antibodies against neutrophil gelatinase-associated lipocalin (NGAL) and development of an antibody-based chemiluminescence immune quantification assay
Jialong QI ; Jia SHAO ; Kuan PENG ; Mingcong HUANG ; Liwen DENG ; Shaowei LI ; Jun ZHANG ; Ningshao XIA ; Ying GU
Chinese Journal of Biochemical Pharmaceutics 2015;37(4):5-9
Objective To obtain monoclonal antibodies ( mAbs ) against neutrophil gelatinase-associated lipocalin ( NGAL ) and a chemiluminescense immune quantification assay based one paired mAbs.Methods Six-to-eight weeks old female BALB/c mice were immunized with the purified recombinant human NGAL antigen( rhNGAL) that was produced by the Escherichia coil expression system.The spleen was fused with hybridoma for screening anti-NGAL monoclonal antibodies by indirect ELISA.Western blot was implemented to identify the reactivity with native NGAL. Results The rhNGAL antigen was found to form disulfide cross-linked dimers and present excellent immunogenicity.The reaction titer of the immune serum of NGAL immunized mice was about 106.Thirty mAbs were screened by indirect ELISA, hereinto;the EC50 values of mAb23C12 and 38D10 were 0.034 g/mL, 0.022 g/mL respectively.The antibodies pair, 38D10/23C12-SAE labeled with AcridiniumEster(AE), were shown to work well in chemiluminescense immune response quantitative detection which was screened by NGAL standardand clinical urine samples.This detection can resolve positive and negative samples with a statistically significant difference (P<0.0001).And the correlation coefficient R2between NGAL quantitative results and that of the Abbott's NGAL chemiluminescence immune assay kit was greater than 0.97.The detection linear range was 10-1500 ng/mL, analytical sensitivity of the method was 0.63 ng/mL.Conclusion Highly purified rhNGAL antigen and specific anti-NGAL monoclonal antibodies are generated in this study.The detection capability of method is comparable with that of the international commercial kit.
3.Relationship between apolipoprotein E, D10S1225 polymorphisms and late-onset Alzheimer's disease.
Tai-song ZHANG ; Hua-qiao WANG ; Wei-yi WANG ; Yun-shao HE ; Shao-kuan HUANG
Chinese Medical Journal 2006;119(4):294-299
BACKGROUNDThere were some papers published in the Jonrnal of Science, December 2000 suggesting that one or more important susceptibility genes for late onset Alzheimer's disease (LOAD) were located on the long arm of chromosome 10. Linkage analysis showed maximum lod score close to D10S1225 loci, which indicated the loci might contribute to the etiology of Alzheimer's disease (AD).
METHODSFifty-nine LOAD patients and 107 controls were recruited. Apolipoprotein E (ApoE) genotypes were determined by reverse dot blotting hybridization assay. The D10S1225 was genotyped by 12% nondenaturing polyacrylamide gels electrophoresis and analyzed by silver staining. Statistical analysis was used to compare genotype and allele distributions between LOAD group and control group for ApoE and D10S1225 polymorphisms.
RESULTSApoE epsilon 4 was significantly higher in LOAD group in comparison with the control group (chi(2) = 6.530, P = 0.011). Seven different alleles of D10S1225 have been identified. The length of these gene fragments were 178 bp, 181 bp, 184 bp, 187 bp, 190 bp, 193 bp, and 196 bp, respectively. A total of 21 different genotypes were observed. There was no relationship between D10S1225 polymorphism and LOAD (chi(2) = 4.488, P > 0.05). Conclusion This study suggests that ApoE epsilon 4 is a risk factor for LOAD, however, the results indicated that there is not any possible linkage for disequilibria with a nearby AD risk gene near D10S1225.
Aged ; Aged, 80 and over ; Alleles ; Alzheimer Disease ; genetics ; Apolipoproteins E ; genetics ; Female ; Genotype ; Humans ; Linkage Disequilibrium ; Male ; Middle Aged ; Polymorphism, Genetic
4.Application of Montreal Cognitive Assessment in screening mild cognitive impairment in elderly patients
Hai-Yuan LI ; Yan-Ping WANG ; Shao-Kuan HUANG ; Shao-Qing YANG ; Sheng-Qiang CHEN ; Yong-Ping DENG ; Zhi-Chao CHEN
Chinese Journal of Neuromedicine 2009;8(4):376-379
Objective To assess the value of Montreal Cognitive Assessment(MoCA)in screening mild cognitive impairment(MCI)in elderly patients.Methods Thirty-two elderly patients with MCI and 50 healthy elderly subjects were examined with neuropsychological tests using Mini-mental State Examination(MMSE)and the MoCA. Results With a cut-off score of 26,the MoCA had a sensitivity of 96.87%and specificity of 76%for detecting MCI,and the sensitivity and specificity of MMSE were 56.25%and 96%,respectively.For the MoCA sub-items,the elderly patients with MCI had significantly lower scores than the healthy elderly in all the cognitive function measures (P<0.05)except for abstraction and fixed orientation.In MMSE sub-items,significant differgnces were found between the two groups in only calculation/attention and delayed recall(P<0.05).Conclusions The MoCA is a highiy sensitive scale for screening MCI in elderly patients,which allows comprehensive assessment of the cognitive function of MCI patients and is applicable in detecting MCI in patients with normal MMSE results.
5.Effects of High-frequency Repetitive Transcranial Magnetic Stimulation in Different Frequencies on Upper Limb Function after Ischemic Stroke
Chang-lin XIAO ; Cui-huan PAN ; Yan CHEN ; Nan HU ; Shao-kuan HUANG ; Qian LI ; Zhen FU ; Xiu-jun OU ; Li-juan LUO
Chinese Journal of Rehabilitation Theory and Practice 2019;25(5):557-563
Objective:To observe the effect of 3 Hz and 10 Hz repetitive transcranial magnetic stimulation (rTMS) on upper limb motor function and activities of daily living in patients with ischemic stroke. Methods:From June, 2016 to September, 2017, 60 inpatients with ischemic stroke were randomly divided into sham rTMS group (