1.Changes in electrocardiographics of 215 patients after transcatheter closure of ventricular septal defect
Hong SHAO ; Yuying WANG ; Jing ZOU
Chinese Journal of Interventional Cardiology 2003;0(05):-
Objective To explore the characteristics of electrocardiographic (ECG) changes after transcatheter closure of ventricular septal defect (VSD). Methods Changes in ECGs of 215 patients after transcatheter closure of VSD from 2002 to 2004 were studied. Results The ECGs of all 215 patients were normal before operation while after operation, ECGs remained normal in 137 patients but 78 patients turned out to be abnormal. Transient atrioventricular interference dissociation occurred in 17 patients and their ECGs recovered to normal after 5 days. Left anterior bundle branch block occurred in another 17 patients and 15 of them returned to normal state 6 days after the procedure. Either right bundle block or left one occurred in 22 patients and their ECGs remained abnormal until they were discharged from the hospital. Advanced or Ⅲ? atrioventricular block occurred in 11 patients, 9 of them returned to normal state, but 2 patients required pocemaker implantation. Conclusion Transcatheter closure of VSD interferes the cardiac conducting system. Most patients can restore normal state, but few may leave behind sequelce.
2.P-450-dependent epoxygenase pathway of arachidonic acid is involved in myeloma-induced angiogenesis of endothelial cells.
Jing, SHAO ; Qiubai, LI ; Hongxiang, WANG ; Fang, LIU ; Jiangang, JIANG ; Xiaojian, ZHU ; Zhichao, CHEN ; Ping, ZOU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(5):596-601
P-450-dependent epoxygenase pathway of arachidonic acid and the products of epoxyeicosatrienoic acids (EETs) have been demonstrated to be involved in angiogenesis and tumor progression. This study examined the expression of EETs and the role of the pathway in the angiogenesis of multiple myeloma (MM). MM cell lines of U266 and RPMI8226 were cultured, and the EETs levels (11, 12-EET and 14, 15-EET) in the supernatant were determined by ELISA. Human umbilical vein endothelial cells (HUVECs) were cultured and used for analysis of the angiogenesis activity of the two MM cell lines, which was examined both in vitro and in vivo by employing MTT, chemotaxis, tube formation and matrigel plug assays. 11, 12-EET and 14, 15-EET were found in the supernatant of the cultured MM cells. The levels of the two EETs in the supernatant of U266 cells were significantly higher than those in the RPMI8226 cell supernatant (P<0.05), and the levels paralleled the respective angiogenesis activity of the two different MM cell lines. 17-octadecynoic acid (17-ODYA), as a specific inhibitor of P450 enzyme, suppressed HUVECs proliferation and tube formation induced by MM cells. Furthermore, 17-ODYA decreased the EET levels in the supernatant of MM cells. These results suggest that EETs may play an important role in the angiogenesis of MM, and the inhibitor 17-ODYA suppresses this effect.
3.Correlation of pathology in chronic hepatitis B to viral markers in serum and hepatic tissue.
Jing HE ; Shao-jie XIN ; Jing-min ZHAO ; Song-shan WANG ; Shao-li YOU ; Zheng-sheng ZOU
Chinese Journal of Experimental and Clinical Virology 2005;19(3):264-266
OBJECTIVETo investigate the relation of the viral markers in serum and those expressed by hepatocytes to pathological lesions of hepatic tissue in patients with chronic hepatitis B.
METHODSThe relation of viral markers including HBsAg, HBsAb, HBeAg, HBeAb, HBcAb and HBV DNA in serum of 647 patients with chronic hepatitis B and HBsAg, HBcAg expressed by hepatocytes in 418 of these patients to pathological lesions of hepatic tissue was determined.
RESULTSViral markers in serum and those expressed by hepatocytes in patients with chronic hepatitis B were closely correlated with pathological lesions of hepatic tissue.
CONCLUSIONThe degree of inflammation and fibrosis in hepatic tissue is milder in serum HBsAg, HBeAb, HBcAb positive and HBV DNA negative patients but more serious in those with negative hepatocytic expression of HBsAg and HBcAg. HBV DNA is not significantly associated with pathological lesions of hepatic tissue.
Adolescent ; Adult ; Child ; Child, Preschool ; DNA, Viral ; blood ; genetics ; Female ; Hepatitis B Core Antigens ; blood ; Hepatitis B Surface Antigens ; blood ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; genetics ; immunology ; physiology ; Hepatitis B, Chronic ; blood ; pathology ; virology ; Host-Pathogen Interactions ; Humans ; Infant ; Infant, Newborn ; Liver ; pathology ; virology ; Male ; Middle Aged ; Young Adult
4.2-year clinic effect of fluoride drop on dental caries prevention of primary teeth.
Shao-min LI ; Xu FAN ; Jing ZOU
West China Journal of Stomatology 2005;23(2):136-137
OBJECTIVETo study the preventive effects of fluoride drop on dental caries for children.
METHODSThe comparative method was carried out in 1 200 children aged from 3 to 4. The subjects were divided into three groups: fluoride drop group, fluoride toothpaste group and control group. The research period was 2 years.
RESULTSThe results demonstrated that new dfs was 1.35 +/- 0.57 in the fluoride drop group after one year, 1.85 +/- 0.57 after two years. The new dfs was 1.53 +/- 1.09 in the fluoride toothpaste group after one year and 2.75 +/- 1.33 after two years. There was no significant difference of the dfs reduction between fluoride drop and toothpaste group.
CONCLUSIONFluoride drop is effective in the prevention of dental caries in Chinese kindergarten and primary school children.
Ambulatory Care Facilities ; Cariostatic Agents ; therapeutic use ; Child ; DMF Index ; Dental Caries ; prevention & control ; Female ; Fluorides ; therapeutic use ; Humans ; Male ; Tooth, Deciduous ; Toothpastes
5.Analysis of oral microflora early colonized in infants.
Jing ZOU ; Xue-dong ZHOU ; Shao-min LI
West China Journal of Stomatology 2004;22(2):126-128
OBJECTIVETo observe the dynamic changes of oral microflora early colonized in infants.
METHODSThe oral swab samples for the study were taken in 1 day, 1, 3, 6, 9, 12 months after birth from 12 healthy neonates. By choosing suitable diluted concentration, the samples were incubated aerobically, facultative anaerobically and anaerobically. The strains were identified by observing colony characteristics, Gram staining and biochemical tests.
RESULTSS. salivarius was the most frequent microflora, followed by S. mitis, S. sanguis, S. gordonii and S. mutans occurred in oral cavity after tooth eruption. Veillonella spp. can be detected in oral cavity of 1-month-old babies, A. odontolyticus was isolated from 8.3% infants of more than 3 months old. L. acidophilus maintained the lower prevalence in oral cavity of babies. Leptotrichia buccalis and Capnocytophaga spp. occurred in oral cavity of some dentate infants.
CONCLUSIONS. solivarius and S. mitis are predominant species in oral cavity of the infants, Veillonella spp. is the first and the most anaerobic species appeared in oral cavity of healthy babies. A. odontolyticus is the first actinomyces detected in oral cavity. With the increasing months, kind and amount of microflora increase dramatically.
Actinomyces ; isolation & purification ; Colony Count, Microbial ; Female ; Humans ; Infant ; Infant, Newborn ; Longitudinal Studies ; Male ; Mouth ; microbiology ; Mouth Mucosa ; microbiology ; Saliva ; microbiology ; Streptococcus ; classification ; isolation & purification ; Streptococcus mitis ; isolation & purification ; Streptococcus mutans ; isolation & purification ; Streptococcus sanguis ; isolation & purification ; Veillonella ; isolation & purification
6.Clinical value of cupping spot effect.
Chang-Chun JI ; Li-Ping HUANG ; Gai-Qin YANG ; Jing-Yu ZHAO ; Wei ZOU ; Xiao-Chuan GUO ; Shao-Ming LIU
Chinese Acupuncture & Moxibustion 2014;34(12):1217-1220
The cupping spot is considered as one kind of skin change due to cupping treatment. With literature regarding cupping spot, the influencing factors and value of cupping spot in clinical diagnosis and treatment were analyzed, which could make a further exploration on the action mechanism of cupping treatment. The literature showed that the formation of cupping spot was related with cupping temperature, pressure, cup-retaining time, cupping area, individual difference and health condition, etc; cupping spot had the ability to assist diagnosis, prevent disease, cure disease and evaluate clinical efficacy. Previous studies on cupping spot have already made some progress, and played a positive significance on finding cupping rule and studying its mechanism. However, the research for this area is still in the primary stage, which needed deeper study to reveal scientific connotations of cupping spot.
Acupuncture Therapy
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instrumentation
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Humans
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Skin
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anatomy & histology
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blood supply
7.Effects of fluoride drop or fluoride ion infiltration in toddler period on demineralization of first permanent molar of children.
Ye ZHANG ; Jing ZOU ; Shao-min LI ; Jun-qi LING
West China Journal of Stomatology 2005;23(4):289-291
OBJECTIVETo observe the effect of fluoride drop or fluoride ion infiltration on demineralization of the first permanent molar of children.
METHODS45 children applied by fluoride drop over 3 years in Chengdu and 40 controls were selected. 45 children applied by fluoride ion infiltration more than 3 years in Xiamen and 40 controls were selected in this study. Demineralization of the different sites of the first permanent molar was evaluated by KaVo DIAGNOdent. The effect of FD and FII in toddler period on demineralization of the first permanent molar was evaluated.
RESULTSEarly demineralization of the first permanent molar in children without fluoride drop were more severe than that of children with fluoride drop in toddle period (P < 0.01). There was no significantly difference between demineralization of first permanent molar in children with fluoride ion infiltration and control group (P > 0.05).
CONCLUSIONFluoride drop in toddle period can help the remineralization of newly erupted first permanent molar of children. There is no effect of fluoride ion infiltration on demineralization of newly erupted first permanent molar of children.
Child ; Child, Preschool ; Fluorides ; Humans ; Molar ; Phosphates ; Tooth Eruption
8.Correlations of P21-activated kinase 1 expression to clinicopathological features of gastric carcinoma and patients' prognosis.
Juan-Xia WANG ; Yong-Ning ZHOU ; Shao-Jing ZOU ; Tao-Wen REN ; Zhi-Yi ZHANG
Chinese Journal of Cancer 2010;29(7):649-654
BACKGROUND AND OBJECTIVERecent studies proved that P21-activated kinase 1 (PAK1) is highly expressed in many kinds of tumor and plays an important role in genesis, development, and metastasis of tumor. We aimed to detect the expression of PAK1 in gastric carcinoma and to analyze its relationship with clinicopathological features and prognosis of gastric carcinoma.
METHODSTissue microarray and immunohistochemical staining were performed to detect PAK1 in paraffin specimens of 189 gastric carcinomas, 54 paracancer tissues, 40 lymph nodes and 30 healthy tissues. Clinicopathologic features and follow-up data of the patients were analyzed by the Chi2 test and the Kaplan-Meier method.
RESULTSPositive rate of PAK1 was 73.0% in gastric carcinoma, 57.4% in paracancer tissues and 23.3% in healthy controls (Chi2 = 29.364, P < 0.05). Expression of PAK1 was significantly correlated with tumor size, tumor differentiation, lymph node metastasis, Lauren classification and invasive depth (all P < 0.05). The positive rate of PAK1 was significantly higher in primary gastric carcinomas than in metastatic lymph nodes (75.0% vs. 52.5%, Chi2 = 4.381, P < 0.05). Survival analysis using the Kaplan-Meier method showed that the expression of PAK1 was a predictor for poor prognosis of the patients with gastric carcinoma (Chi2 = 6.857, P < 0.01).
CONCLUSIONSExpression of PAK1 is an early molecular event in the tumorigenesis of gastric carcinoma. It is also closely correlated the development of gastric carcinoma and the patients' prognosis.
Adult ; Aged ; Female ; Follow-Up Studies ; Humans ; Lymph Nodes ; metabolism ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Staging ; Prognosis ; Risk Factors ; Stomach Neoplasms ; metabolism ; pathology ; Survival Rate ; Tumor Burden ; p21-Activated Kinases ; metabolism
9.Role of Baicalein in the regulation of proliferation and apoptosis in human myeloma RPMI8226 cells.
Qiu-bai LI ; Yong YOU ; Zhi-chao CHEN ; Jian LÜ ; Jing SHAO ; Ping ZOU
Chinese Medical Journal 2006;119(11):948-952
Apoptosis
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drug effects
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Arachidonate 12-Lipoxygenase
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analysis
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physiology
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Cell Cycle
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drug effects
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Enzyme Inhibitors
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pharmacology
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Flavanones
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pharmacology
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Humans
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Lipoxygenase Inhibitors
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Multiple Myeloma
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drug therapy
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pathology
10.Study on the overlapping growth of hepatoma cells in vitro.
Hongbing WANG ; Xiaobing ZOU ; Yanzi YANGBEN ; Jing SHAO ; Xiaodong YANG ; Weidong GAO
Journal of Biomedical Engineering 2008;25(3):652-657
This study inquired into the formation of the "overlapping growth" of hepatoma cells through quantitative characterization on the growth of hepatoma cells in situ by means of morphological observation, Image Tool computer analytic system, statistical analysis as well as the experimental methods of cell mechanics and biochemistry. The results were as follows: (1) The ability of hepatoma cells to regulate cell morphological deformation was better than that of hepatic cells; (2) While we were using micropipette aspiration technique to suck the "overlapping growth plaque" of hepatoma cells, the "overlapping growth plaque" fell off from the substrate, leaving a blank area; (3) Integrin expression of hepatoma cells was more obvious than that of hepatic cells; (4) Fibronectin (Fn) down-regulated the integrin expression in the hepatoma cells cultured on the Fn coated surface, enhanced the cells' adhesion ability and morphological stability, but reduced the formation and aggregation of the round cells. These results indicated (1) The so-called overlapping growing area was actually formed by many closely arrayed and piled round cells; (2) The production of round cells may be caused by integrin abnormal expression and the effect on the hepatoma cells adhesion stability; (3) The formation of "overlapping growth plaque" in hepatoma cells is related to the round cells' congregation induced by the high frequency morphological transformation.
Carcinoma, Hepatocellular
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metabolism
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pathology
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Cell Adhesion
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Cell Proliferation
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Fibronectins
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metabolism
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Humans
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Image Processing, Computer-Assisted
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Integrin beta Chains
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biosynthesis
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genetics
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Liver Neoplasms
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metabolism
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pathology
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Tumor Cells, Cultured