Objective To provide reliable and scientific data for supervision assessment and control of regional ambient air quality in Beijing through the investigation of ambient air quality in Chaoyang District. Methods Three local regions of Chaoyang Park Dabeiyao and Dongzhimen were selected as the measurement areas the determinations of air quality of consecutive 5 days and 4 times a day8 00 12 00 16 00 20 00 were conducted 6 items were included PM10 CO NO2 SO2 and negative ion concentration. Results The daily averages of CO and SO2 were lower than level 2 standard limitation for 5 days’ measurement in 3 regions. 2 of 5 days the daily average of NO2 in Dongzhimen exceeded the standard limitation0.01 mg/m3. 2 of 5 days the daily average of PM10 in 3 regions exceeded the standard limitation  0.01- 0.04 mg/m3. Conclusion Preliminary analysis was carried out based on ambient air quality data in 3 local regions the air pollution often emerged on some days in Beijing. The supervision and control should be conducted according to the characters of regional environmental quality pollution and meteorology.

Objective To explore the characteristics of electrocardiographic (ECG) changes after transcatheter closure of ventricular septal defect (VSD). Methods Changes in ECGs of 215 patients after transcatheter closure of VSD from 2002 to 2004 were studied. Results The ECGs of all 215 patients were normal before operation while after operation, ECGs remained normal in 137 patients but 78 patients turned out to be abnormal. Transient atrioventricular interference dissociation occurred in 17 patients and their ECGs recovered to normal after 5 days. Left anterior bundle branch block occurred in another 17 patients and 15 of them returned to normal state 6 days after the procedure. Either right bundle block or left one occurred in 22 patients and their ECGs remained abnormal until they were discharged from the hospital. Advanced or Ⅲ? atrioventricular block occurred in 11 patients, 9 of them returned to normal state, but 2 patients required pocemaker implantation. Conclusion Transcatheter closure of VSD interferes the cardiac conducting system. Most patients can restore normal state, but few may leave behind sequelce.

Objective: To observe the therapeutic effects of warm-needling technique on peripheral facial paralysis. Methods: Warm-needling technique and electroacupuncture were employed to treat 30 cases of facial paralysis, respectively. The same acupoints, Cuanzhu(BL 2)towards Yuyao(Ex-HN 4), Yingxiang(LI 20) towards Xiaguan(ST 7), Taiyang(Ex-HN 5)towards Xuanlu(GB 5), Dicang(ST 4) towards Jiache(ST 6), and Chengjiang(CV 24) towards Daying(ST 5), were used. Results: After 33 treatments, the warm-needling technique has an effective rate of 83.3%, while the electroacupuncture 67.7%. Conclusion: The therapeutic effect of warm-needling technique was better than that of electroacupuncture method.

Carcinoma, Renal Cell ; complications ; Humans ; Kidney Neoplasms ; complications ; Lymphoma, Non-Hodgkin ; complications ; Male ; Middle Aged

Objective To explore the molecular mechanism of hypophrenia induced by Down syndrome (DS).Methods Ts65Dn mice were used as DS animal model.Three female mice and three male mice of three to twelve weeks old were mated.Among the 17 first-generation mice alive,five mice remained Ts65Dn trisomy and 12 mice were normal.Five Ts65Dn mice and five normal mice were selected randomly as Ts65Dn group and control group,and bred till 16 to 18 weeks old for experiments.Differential proteins in hippocampus of mice were tested by isobaric tags for relative and absolute quantitation (iTRAQ).Expressions of the differential proteins in Ts65Dn group were detected compared with those in control group.Results A total of 2805 proteins were identified in hippocampus of Ts65Dn group and control group,and significant differences were observed in the expressions of 374 proteins.Compared with those in control group,expressions of 195 proteins increased and 179 reduced in Ts65Dn group.Sorted by P value from low to high,the seven proteins with the lowest P value were uncharacterized protein C2orf47 homolog,isoform 2 of filamin A-interacting protein 1-like,zinc finger protein,isoform 1 of pericentriolar material 1 protein,SEC23 interacting protein,BAG family molecular chaperone regulator 3 and serpin H1.Conclusions Differential proteins are observed in hippocampus of Ts65Dn mice,perhaps closely correlating to neurological defects.The new technology of iTRAQ helps to screen and identify differential proteins in hippocampus.

Objective According to the lack of timeliness in the current domestic disease notification system,an effective solution is proposed to solve the problem.Methods Based on research and analysis of the health information exchange standards HL7 (Health Level 7),which is widely used in the world,a solution on the base of HL7 standards is proposed in this study,to store,transfer,analyze and publish data.Results The solution allows medical institutions and the centers for disease control connect seamlessly,ensuring the data transfer in time,and publishing the data through Web service.Conclusion The solution can keep the people abreast of the latest status of the epidemic,and do their own protection as well as promote social stability.

Objective To investigate the characteristics of marital quality and social support status of the patients with breast cancer during the period of chemotherapy and the correlation of both factors.Methods Seventy-seven patients with breast cancer were divided into beginning-, middle-and final-staging groups of chemotherapy.And they were studied with Olson marital quality questionnaire and Social Support Rating Scale.Results The total scores of marital quality and other factors were the lowest in the mid-chemotherapy group.Among three groups,the mean of the objective support and availability were gradually increased with the chemotherapy going.The score of objective support was higher in the final than beginning chemotherapy groups (P =0.009).There was a positive correlation between marital satisfaction and the objective support (r =0.248, P =0.036), and between the total score of quality of marriage and subjective support (r =0.286, P = 0.015).Conclusion The marital quality of breast cancer patients receiving chemotherapy is poor,and subjective or and objective support might improve the patient's marital quality.

Objective: To establish a method of restriction fragment length polymorphism (RFLP) to determine the origin of sika deer bones.Methods: The DNA in the bone samples was extracted after decalcification, and then amplified using polymerase chain reaction (PCR).The origin of the samples was further identified using RFLP analysis.Results: The bone samples of sika deer and red deer could be distinguished from those of pig, bovine and dog by PCR.And the samples of sika deer and red deer could be further distinguished by RFLP through the analysis of the length of restriction enzyme XbaI.Conclusion: A RFLP method is established to determine the origin of sika deer bones.

Objective To explore the optimal condition for the differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in vitro by using DMSO.Methods SD rat MSCs were isolated from rat bone marrow and cultured,then induced by 1.0% DMSO for 24 h.The cultured cells were observed through phase-contrast microscopy.Immunohistochemical technique and laser scanning confocal microscopy(LSCM) were used for detecting the expression of desmin,?-sarcomeric actin and C-TnT.The ultrastructures were examined with transmission electron microscopy.Results MSCs induced by DMSO were identified by the positive staining for desmin,?-sarcomeric actin and C-TnT.Transmission electron microscopy showed that paralleled myofilaments and a lot of rough endoplasmic reticulum were formed.Conclusion MSCs can be induced to differentiate into cardiomyocyte-like cells by DMSO.

Objective To Study on CXCR1/CXCR2 antagonist G31P anti-inflammatory reaction mediated by neutrophils.Methods Detect whether G31P can block chemotaxis of neutrophils induced by human IL-8 and inhibit the release of IL-8 by epithelia of segmental bronchus;establish HEK293 cell line transfected by pcDNA3.0-CXCR1 ,2,4 and detect the chemotaxis of IL-8 for HEK293 ;establish the experi-mental model of pneumonia induced by the P.aeruginosa,take count of the nucleated cells in the bronchoal-veolar lavage fluid(BALF),analyze myeloperoxidase(MPO) of lung tissue and observe the histopathology changing of it.Results G31P can inhibit the chemotaxis for neutrophils and transfected HEK293 cell line,inhibit the A549 releasing of inflammatory mediators;the proportion of neutrophils declines in G31P treat-ment group,pathology examination appears clear discrepancy.Conclusion G31P can block the chemotaxis of chemotactic factor with ELR+ CXC to neutrophils,block the combination of chemotactic factor with its re-ceptor CXCR2,block the CXCR2 on the surface of alveolar epithelia and vascular endothelial cells.Accordingly,neutrophils recruiting to topoinflammation can be prevented.