Hypoxic preconditioning (HPC) refers to exposure of organisms, systems, organs, tissues or cells to moderate hypoxia/ischemia that is able to result in a resistance to subsequent severe hypoxia/ischemia in tissues and cells. The effects exerted by HPC are well documented. The original local in situ (LiHPC) is now broadened to remote ectopic organs-tissues (ReHPC) and extended crossly to cross pluripotential HPC(CpHPC) induced by a variety of stresses other than hypoxia/ischemia, including cancer, for example. We developed a unique animal model of repetitive autohypoxia in adult mice, and studied systematically on the effects and mechanisms of HPC on the model in our laboratory since the early 1960s. The tolerances to hypoxia and protection from injury increased significantly in this model. The adult mice behave like hypoxia-intolerant mammalian newborns and hypoxia-tolerant adult animals during their exposure to repetitive autohypoxia. The overall energy supply and demand decreased, the microorganization of the brain maintained and the spacial learning and memory ability improved but not impaired, the detrimental neurochemicals such as free radicals down-regulated and the beneficial neurochemicals such as adenosine(ADO) and antihypoxic gene(s)/factor(s) (AHGs/AHFs) up-regulated. Accordingly, we hypothesize that mechanisms for the tolerance/protective effects of HPC are fundamentally depending on energy saving and brain plasticity in particular. It is thought that these two major mechanisms are triggered by exposure to hypoxia/ischemia via oxygen sensing-transduction pathways and HIF-1 initiation cascades. We suggest that HPC is an intrinsic mechanism developed in biological evolution and is a novel potential strategy for fighting against hypoxia-ischemia and other stresses. Motivation of endogenous antihypoxic potential, activation of oxygen sensing--signal transduction systems and supplement of exogenous antihypoxic substances as well as development of HPC appliances and HPC medicines such as AHFs are encouraged based on our basic research on HPC. HPC may result in therapeutic augmentation of the endogenous cytoprotection in hypoxic-ischemic or suffering from other diseases' patients. Evolutionary consideration of HPC and clinical implications of HPC are both discussed to guide future research. The product of AHF is expected to be one of the most effective first aid medicines to rescue patients in critical condition. HPC is beginning to be used in surgery and is expected to be developed into a feasible adaptive medicine in the near future.
Animals ; Brain ; physiology ; Disease Models, Animal ; Hypoxia, Brain ; physiopathology ; Hypoxia-Inducible Factor 1 ; Ischemic Preconditioning ; Mice ; Signal Transduction

ObjectiveTo study the application of glucose descriptive chart in monitoring of stress hyperglycemia,and provide continuous,dynamic change of blood glucose levels for clinic. Methods80 patients with stress hyperglycemia who adopted intensive insulin therapy were randomly divided into the experimental group and the control group with 40 cases in each group.The experimental group used glucose descriptive charts to record blool glucose,the control group used the traditional registration form to record blood glucose.The incidence of hypoglycemia and evaluation of medical staff for the glucose descriptive chart were compared between two groups. ResultsThe incidence of hypoglycemia in the experimental group was significantly lower than the control group.The medical staff considered that glucose descriptive chart helped understand the dynamic change of blood glucose,and provided a more comprehensive basis for regulating the use of insulin. ConclusionsApplication of glucose descriptive chart in monitoring of stress hyperglycemia can provide continuous and dynamic change of blood glucose,which facilitates the medical staff to monitor the blood glucose and decrease the incidence of hypoglycemia.

Objective To study the chemical constituents of Smilax china.Methods The compounds were isolated by silica gel,Sephadex LH-20,ODS chromatography,and semi-preparative HPLC.Their structures were elucidated on the basis of MS and NMR spectroscopic analyses.Results Seven flavonoids and four stilbenes were isolated and identified as dihydrokaempferol-5-O-?-D-glucoside(Ⅰ),engeletin (Ⅱ),isoengeletin(Ⅲ),dihydroquercetin-3-O-glycoside(Ⅳ),3,5,7,3',5'-pentahydroxy-flavanonol (Ⅴ),astilbin(Ⅵ),quercetin-3'-Oglycoside(Ⅶ),piceid(Ⅷ),scirpusin A(Ⅸ),resveratrol(Ⅹ),and oxyresveratrol(Ⅺ).Conclusion CompoundsⅣ—Ⅸare isolated from this plant for the first time,among which the ~(13)C-NMR data of compoundⅨis reported firstly.

Objective To evaluate the degree of erectile dysfunction in the benign prostate hyperplasia (BPH) of men with moderate and severe lower urinary tract symptoms (LUTS) .Methods Total 140 patients with moderate and severe LUTS of BPH were investigated using the International Prostate Symptom Score (IPSS) and International Index of Erectile Function-5 (IIEF-5). Erectile dysfunction was less than 21 scores, but erection was more than 21 scores. Regression analysis was used to determine the degree of any correlation among age, the total IPSS, the moderate and severe IPSS score and the various erectile function scores.Results The mean age of the patients was (69 2?7 18) years. The mean total IPSS score was 23 3?7 7, consisting of 45 patients (32%) for moderate IPSS score and 95 patients (68%) for severe IPSS score. The mean IIEF-5 score was 6 8?8 02. There were erection patients in 11 cases (7 9%) and erectile dysfunction patients in 129 cases (92 1%). On the other hand, 41 cases (91%) for moderate IPSS score and 88 cases (93 6%) for severe IPSS score exists erectile dysfunction. There were no significant correlation among IIEF-5 score and patients age (?=0 417), the total IPSS (?=0 235), the moderate (?=0 07) and severe IPSS score (?=0 332). Conclusions About 90% BPH men complicated with LUTS have erectile dysfunction. Erectile dysfunction is related to age and the extent of LUTS. LUTS is not an important factor of erectile dysfunction. The high morbidity of erectile dysfunction in BPH men with LUTS should be consider in the treatment of their urinary symptoms.

TA method for the content determination of methionine sulfoxide and methionine sulfone in compound amino acid injection (18AA-II) was established in order to investigate their level in 155 batches of this product, and to explore the reason for the generation of these two impurities.The determination was performed on an Agilent Poroshell 120 EC-C18 column with mobile phases of sodium acetate/tetrahydrofuran solution (A) and sodium acetate solution -acetonitrile-methanol (B, 200∶400∶400) (gradient elution) at the flow rate of 0.5 mL/min.The excitation wavelength and the emission wavelength of the fluorescence detector were 233 nm and 441 nm, respectively.The column temperature was 40 °C, and the injection volume was 8 μL.The contents of methionine sulfoxide and methionine sulfone from 155 batches of compound amino acid injection (18AA-II) was determined using this method, and the residual oxygen content was detected by headspace gas analyzer.The results showed that the linear range of methionine sulfoxide and methionine sulfone were 0.128 1-10.250 0 μg/mL (r = 0.999 9) and 0.261 0-10.440 0 μg/mL (r = 0.999 8), respectively.The limits of quantitation were 0.13 μg/mL and 0.26 μg/mL, respectively; the limits of detection were 0.04 μg/mL and 0.09 μg/mL, respectively.RSDs of precision, stability and repetitive test were all lower than 1.3%.The recoveries ranged 98.00%-100.79% (RSD = 1.15%, n = 9) and 98.19%-102.31% (RSD = 1.33%, n = 9).The content level of oxidized related substances from different manufacturers showed significant difference, showing relevance with the residual oxygen content to some extent, yet no significant correlation with the added amount of antioxygen (sodium pyrosulfite).The method is validated to be useful for the content control of methionine sulfoxide and methionine sulfone in compound amino acid injection (18AA-II).It is quite necessary to include the determination of oxidized related substance into the quality specification.Manufacturers should strengthen the control of remaining oxygen in their products.

Benzene ; analysis ; chemistry ; Benzene Derivatives ; blood ; urine ; Humans

Objective To study the effect of suppressing metastasis between KAI1 and ME491 on pancreatic cancer.Methods Normal pancreases and pancreatic cancers were analyzed using Northern blot,in situ hybridization and immunohistochemistry.Results The expression of KAI1and ME491 was higher in pancreatic cancer than that in normal pancreases,KAI1 expression was higher in pancreatic cancer without metastasis than that in pancreatic cancer with metastasis(P0.05).Conclusion The gene of KAI1 may be involved in the metastasis of pancreatic cancer,ME491 gene may have no effect on the metastasis of pancreatic cancer.

Objective To evaluate the application effect of the problem based learning in clinical teaching of orthopedics. Methods We chose sixty five-year program students of Peking univ-ersity health science center as research object. All students were randomly divided into experimental and control groups, with thirty students in each group. Experimental group was given PBL teaching combined with lecture based learning (section-based learning, LBL), while the control group only received the LBL teaching. Two groups were given 8 hours of teaching experiments. After the end of the study, the teaching effect of the two groups was evaluated by the theory course and clinical skills. The questionnaire was distributed to the 2 groups. The scores of both experimental group students and control group students in theory courses and clinical skills were analyzed using SPSS 19.0 software using t-test and x2 test. Result The score of the experimental group was (53.7 ±3.2) in the knowl-edge-based grades exam, while the control group was (52.3±2.2), showing no obvious difference when compared to the control group's grades (P>0.05). The score of the experimental group was (24.0±1.5) in the hands-on technique grades exam, while the control group was (22.3±1.6). The difference in grades showed statistical significance (P<0.05). Feedback survey results showed that the experimental group's teaching satisfaction degree was also significantly higher than the control group, and the dif-ference was statistically significant (P<0.05). Conclusions The application of PBL in clinical teach-ing in orthopedic clinical teaching can improve students' learning interest and self-study ability, and helps to develop students' clinical thinking ability. But the role of PBL teaching in improving students' medical knowledge level is not obvious.

ObjectiveTo examine the attachment style,the anxious and avoidant related attachment in adolescent patients with somatoform disorders.MethodsAll of the 66 patients who were diagnosed as somatoform disorder and 80 controls completed the assessments of common information,experiences in close relationships scales.All the data was analyzed by SPSS13.0.ResultsCompared with control group,somatofonn disorder group had higher ratio of insecure attachment,and there were statistically significant differences between them (29.5％vs 81％,x2 =40.584,P＜0.01 ).The scores of avoidant and anxious related attachment in somatoform group were significant higher than those in control group ( separately 70.51 ± 15.08 vs 62.58 ± 14.18,t =4.449,P ＜0.0l ;64.25 ± 18.91 vs 51.49 ± 14.13,t =3.210,P ＜ 0.01 ).ConclusionThere is a higher incidence of insecure attachment and higher level of anxious and avoidant related attachment in adolescent patients with somatoform disorder.The most common style is dismissing attachment.

Objective To establish and optimize ISSR-PCR systems of Chrysanthemum morifolium and lay the foundation for its genetic diversity research. Methods Based on the analysis of variance, an orthogonal design was used to optimize the ISSR-PCR amplification system on C. morifolium by four factors (Taq polymerase, Mg2+, dNTP, and primer) at three concentration levels, respectively. Results A suitable ISSR reaction system was constructed with the 20 ?L reaction system containing 1.00 U Taq polymerase, 2.00 mmol/L Mg2+, 0.20 mmol/L dNTP, and 0.50 ?mol/L primer. Conclusion ISSR-PCR is significantly influenced by the concentration of Taq polymerase, Mg2+, and dNTP. This ISSR-PCR system could provide clear bands, reliable reaction system, and abundant polymorphisms . It is proved to be suitable for the study of the genetic diversity of C. morifolium