Two patients with coexistence of thyroid disease and suspected Gitelman's syndrome underwent SLC12A3 gene analysis. The results confirmed that both patients were compound heterozygotes of SLC12A3 gene mutation. Three novel variants of SLC12A3 were found in this study. This report suggests that Gitelman's syndrome may coexist with other disorders associated with hypokalemia, such as Graves' disease.

Objective To observe the serum lipid level of patients with branch retinal vein occlusion (BRVO).Methods A total of 71 BRVO patients (BRVO group) were enrolled in this study.The patients included 31 males and 40 females,with an average age of (52.75 ± 10.2) years.All the patients were examined for visual acuity,slit lamp ophthalmoscopy combine with preset lens,fundus color photography and fundus fluorescein angiography (FFA) examination.Seventy-two age-and sex-matched normal subjects were enrolled in this study as control group.The subjects included 32 males and 40 females,with an average age of (53.10±9.5) years.The BRVO and control group were divided into four subgroup which including age with ＜40 years,40-49 years,50-59 years and ≥60 years.The plasma cholesterol and triglyceride level of BRVO group,control group,and age subgroups of BRVO and control group were comparatively analyzed.Results The average plasma cholesterol levels were (4.529±0.100) and (4.274±0.106) mmol/L in BRVO and control group,respectively.There was no difference between two groups (t=-1.738,P＞0.05).The average triglyceride levels were (1.500±0.129) and (1.319±0.095) mmol/L in BRVO and control group,respectively.There was no difference between two groups (t=-1.135,P＞0.05).There was no difference of average plasma cholesterol (t=-1.755,1.850,-1.892,-0.507) and triglyceride (t=0.846,-0.074,-1.288,-1.887) level in age subgroups of BRVO and control subgroup (P＞0.05).Conclusion There is no significant difference of serum lipid level between BRVO patients and controls.

Objective To evaluate the accuracy of multi-slice spiral computed tomography (MSCT) in preoperative TN staging and in judging surrounding mesenteric invasion.Methods 96 cases of patients with colon carcinoma which was historically confirmed were collected.Common practice scanning with inflation and dynamic contrast-enhancement scan were performed preoperatively and the images of plain scan,venous phase and arterial phase of contrast enhancement including multi-planar and curved planar reconstruction were obtained.TN staging and evaluation of surrounding mesenteric infiltration were made by MSCT.Surgical findings and pathological results were compared with MSCT diagnosis.The accuracy,sensitivity,specificity,false negative rate,false positive rate and consistency analysis between them were compared.Results The accuracy,sensitivity,speciality rate,false negative rate and false positive rate of TN staging of colon cancer,as well as mesenteric infiltration determined with MSCT were consistent with the clinical and historical results (Kappa ＞ 0.69,P ＜ 0.01),meaning good consistency.Conclusion MSCT for colon inflation is a useful method for revealing T staging (≤T2-T4),N staging and mesenteric infiltration of colon carcinoma preoperatively.It is also an important noninvasive method to provide surgeons with valuable information for making treatment plan of the tumor.

Child, Preschool ; Humans ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; virology ; Male

Arthritis, Juvenile ; classification ; diagnosis ; therapy ; Child ; Female ; Follow-Up Studies ; Humans ; Male ; Prognosis

Adult ; Antihypertensive Agents ; therapeutic use ; Blood Pressure ; drug effects ; Depression ; complications ; drug therapy ; physiopathology ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hypertension ; complications ; drug therapy ; physiopathology ; Male ; Middle Aged ; Phytotherapy ; Powders ; Treatment Outcome

Objective:To analyze the clinical effect and influencing factors of closed reduction and percutaneous cannulated screw fixation in the treatment of femoral neck fracture in the adolescents, and to provide evidence for surgical treatment of femoral neck fracture in the adolescents. Methods:The clinical effect and influencing factors of 36 cases of femoral neck fracture treated by closed reduction and percutaneous cannulated screw fixation were analyzed. The time of operation, the time of fracture healing evaluated with X-ray image, the evaluation on the function after operation by Harris score of hip joint,and the occurrence of complications of the patients were observed.Results:All the cases were followed up for 6-24 months, average 16 months. One case of all patients had avascular necrosis of the femoral head, and accounted for 2.78% of all the patients;2 cases of all fractures were nonunion, and 5.56%.The remaining 33 cases were completely healed.The HHS-harris hip score results showed that 26 were excellent, 6 cases were good, and 2 cases were poor;the excellent and good rate was 88.89%.Conclusion:Closed reduction and percutaneous cannulated screw fixation in the treatment of adolescent femoral neck fracture is an effective program of operation;timing of operation, good reduction, and rigid internal fixation can decrease the femoral head necrosis and the incidence of complications.

The suppressive effects of T. reglii on mouse cellular and humoral immune responses were studied. Lymphocyte proliferation effect induced with ConA,and PFC and OHS reactions with TD Ag(TNP-SRBC)were effectively suppressed by the drug. But the similar effects on LPS and TI Ag (TNP-SRBC) have not been found. The results shown that target cells of T. reglii effects may be T lymphocytes and the drug may not have a direct effect on B cells. The drug also shown a suppressive effect on IL-2 production by mouse spleen cells. The phenomenon suggests that the suppressive effects of T. reglii on humoral immune response may be indirectly mediated through suppressing helper T cells.

Five fractions,Ⅰ(R_f0.06)、Ⅱ(R_f0.21)、Ⅲ(R_f0.45)、Ⅳ(R_f0.60)、Ⅴ(R_f0.75)have been obtained after HT-toxin from race 1 of Exserohilum turcicum 99-2 isolated by TLC. In all of these fractions,only fractions Ⅱ had specific toxicity to the corn leaves with Ht1 gene. Then fractions Ⅱ-1、Ⅱ-2、Ⅱ-3 were isolated from fractions Ⅱ by HPLC purification,and the bioassay result showed only fraction Ⅱ-3 was toxigentic to corn leaves with Ht1 gene but non-toxigentic to corn leaves without Ht1 gene. Fractions Ⅱ-1、Ⅱ-2 and Ⅱ-3 were scanned by UV-Vis spectrophotometer. It was shown that the fractions Ⅱ-1 and Ⅱ-2 had analogous spectrum,and especially the fraction Ⅱ-3 had a special peak at 300nm.

Objective: To study the chemical constituents in the whole herb of Coreopsis lanceolata and to investigate the antibacterial activity. Methods: The compounds in the whole herb of C. lanceolata were isolated and purified by column chromatography and identified based on spectral analyses (MS, NMR). The antibacterial activities of compounds 1-8 were screened. Results: Fifteen compounds including eight sesquiterpenes were isolated from the chloroform fraction in the whole herb of C. lanceolata and were identified as 1β, 5α-diangeloyloxy-eudesm-(15)-ene (1), 1β, 6α-dihydroxyeudesm-4(15)-ene (2), 10α-hydroxyoplopan-4-one (3), (7R*)-opposit-4(15)-ene-1β, 7-diol (4), (7R*)-opposit-4(15)-ene-1β, 8-diol (5), (7R*)-opposit-4(15)-ene-1β, 11-diol (6), (7R*)-opposit-4(15)-ene-1β, 7α-diol (7), 4(15)-eudesmene-1β, 7α-diol (8), p-hydroxy cinamic acid (9), 3-methoxy4-hydroxy-benzoic acid (10), methyl-p-hydroxybenzoate (11), 3, 4-dihydroxy-benzaldehyde (12), β-sitosterol (13), friedeline (14), and friedelinol (15). Conclusion: Compounds 1-15 are obtained from the whole herb of C. lanceolata for the first time. The bioassays show that the compounds 3 and 4 have the stronger inhibition against Staphylococcus aureus.