BACKGROUND: Cryopreservation can decrease tissue and organ immunogenicity. The effects of cryopreservation on cell immunogenicity are disputed.OBJECTIVE: To investigate the effects of liquid nitrogen cryopreservation on osteoblast immunogenicity. DESIGN: Randomized,controlled ,paired-sample experiment. SETTING: This study was performed in the Laboratory Center, Qilu Hospital Affiliated to Shandong University between July 2003 and March 2004. MATERIALS: Four New Zealand rabbits of either gender were included for this study. 3H-TdR was provided by Nuclear Medicine Institute of Shandong University. METHODS: Osteoblasts were cultured from the tibial periosteum of New Zealand rabbits and then cryopreservated in the liquid nitrogen for 3 months and defrosted. Cryopreservated and thawn osteoblasts were set as cryopreserved group and freshly cultured osteoblasts were set as non-cyropreserved group. Major histocompatibility complex (MHC)-I positive rate was examined by flow cytometry assay prior to and after cryopreservation. Simultaneously, mixed lymphocyte-osteoblast cultures were established. Lymphocyte stimulation index was calculated after counting the flares using β liquid scintilloscope. MAIN OUTCOME MEASURES: MHI-I antigen positive rate and lymphocyte stimulation index prior to and after cryopreservation of osteoblasts. RESULTS: MHI-I antigen positive rate and lymphocyte stimulation index of osteoblasts was significantly higher in the non-cryopreserved group than in the cryopreserved group (P＜0.01). CONCLUSION: The immunogenicity of cryopreserved osteoblasts was significantly decreased. Liquid nitrogen cryopreservation is an ideal method to decrease the immunogenicity of osteoblasts.

Objective To systematically review the efficacy and safety of resina draconis capsule in the treatment of pressure ulcers.Methods We searched the PubMed,Embase,The Cochrane Library,CBM, CNKI,VIP,and WanFang Data from their inception to August 2016. Two reviewers independently screened literature,extracted data and assessed the risk of bias of included studies. The meta-analysis was performed by using RevMan 5.3 software.Results A total of 36 RCTs involving 1902 patients were included. The results of meta-analysis showed that:as for the total effective rate,resina draconis capsule was superior to conventional disinfectant/preservative [RR=1.24, 95%CI(1.15,1.34),P<0.00001] and antibiotics [RR=1.17,95%CI (1.09,1.26),P<0.0001],and there was no significant difference with Shirun Shaoshang cream [RR=1.36, 95%CI(0.88,2.11),P=0.17]. In terms of recovery rate,resina draconis capsule was superior to conventional disinfectant/preservative [RR=1.87,95%CI(1.41,2.49),P<0.00001] and antibiotics [RR=2.11,95%CI (1.77,2.51),P<0.00001],and there was no significant difference with Shirun Shaoshang cream [RR=1.54, 95%CI (1.00,2.38),P=0.05]. As regard to the wound healing time,resina draconis capsule was superior to conventional disinfectant/preservative [MD=-10.50,95%CI(-15.52,-5.48),P<0.0001],antibiotics [MD=-8.59,95%CI(-11.45,-5.73),P<0.00001],and Shirun Shaoshang cream [MD=-7.00,95%CI (-8.34,-5.66),P<0.00001]. No obvious event of adverse effect was reported.Conclusions According to the current evidence,the efficacy of resina draconis capsule is superior to conventional disinfectant/preservative, antibiotics,and Shirun Shaoshang cream in treatment of pressure ulcers.

Objective To explore the effectiveness of da Vinci Robot Xi system-assisted single-incision laparoscopic surgery for treating benign ovarian lesions in children and adolescents.Methods From June 2020 to March 2023,13 cases of benign ovarian lesions were operated by using a specially designed 4-channel single-incision laparoscopic device assisted by the da Vinci Robot Xi system.The docking between the da Vinci Robot and the patient was completed,and the affected ovaries were cut open with a non-powered scissors to expose the tumor tissue.The tumor tissue was removed as completely as possible along the edge of the tumor,and an absorbable suture was used to seal the wound followed by restoring the umbilical structure.Results All the 13 cases underwent ovarian benign lesion resection through a single incision in the umbilical region,without conversion to open surgery.The surgical time was 81-246 min,with an average of 161.4 min.The intraoperative bleeding volume was 5-50 ml,with an average of 21.2 ml.During the operation,2 cases of ovarian torsion and 3 cases of ovarian benign lesion rupture(with postoperative drainage tube placement)were found.Postoperative pathological findings showed 9 cases of mature teratoma,2 cases of serous cystadenoma,and 2 cases of simple cyst.The postoperative hospitalization time was 2-7 d,with an average of 4.2 d.There was 1 case of postoperative incision infection who was cured after antibiotic treatment.The follow-up period was 6-36 months,with an average of 21.1 months.No tumor recurrence or complications occurred,and all the patients had hidden scars on the umbilical incision.The patients and their families were satisfied with the postoperative appearance.Conclusion The application of da Vinci Robot Xi system-assisted single-incision laparoscopic surgery in the treatment of benign ovarian lesions in children and adolescents is safe and feasible,which can be considered as an option for treating such conditions.