Aim To explore the role of prenatal exposure to lipopolysaccharides(LPS)on aortic morphology in the 6-week offspring rats.Methods Twelve pregnant rats were randomly divided into three groups: control group,LPS group,L+P(pyrrolidinedithiocarbamate,PDTC)group.The rats were intraperitoneally administered vehicle,LPS(0.79 mg·kg-1),or LPS plus PDTC(100 mg·kg-1).LPS was given on the 8th,10th and 12th day,where as vehicle and PDTC were given daily from the 8th to the 14th day during gestation.Offspring body weight was measured at 6-week,and histopathological alteration of the thoracic aorta was observed by transmission electron microscopy,the thoracic aorta mRNA and protein expression of connexin(Cx)molecules including Cx37,Cx40,Cx43 and Cx45 in offspring rats were detected by real time PCR,Western blot and confocal laser-scanning microscope.Results Body weight at 6-week offspring rats was significantly higher in LPS group than in control group whatever male or female(P<0.01);and that in L+P group was significantly lower than in LPS group in male rats(P<0.05),but not in female rats.The LPS group thoracic aortas exhibited lesions,including impaired endothelial cells,and the vascular smooth muscle cells were arranged disorder and migration and proliferation;the number of gap junction was increased and longer than control group;the pathological changes were improved to some extent in L+P group.Cx43 mRNA and protein expression in LPS group was obviously lower than in control group(P<0.05),which could be partly reversed in L+P group(P<0.05).There was no significant difference in Cx37,Cx40 or Cx45 mRNA or protein expression among each group.The results of protein expression with confocal laser-scanning microscope was the same as those of Western blot.Conclusion Maternal LPS exposure during pregnancy leads to vascular changes in 6-week offspring rats,which might last until neonatal stage,and is closely correlated to hypertension in adult offsprings.

With the development of clinical related disciplines, the update and establishment of relevant standards/guidelines at home and abroad, GBZ 185-2006 Diagnostic Criteria for Occupational Medicamentose-like Dermatitis due to Trichloroethylene (hereinafter referred to as “GBZ 185-2006”) was unable to meet clinical needs. Therefore, the GBZ 185-2006 was revised based on the principles of evidence-based medicine, in accordance with relevant laws/regulations and relevant standards/guidelines in combination with review of research data on occupational medicamentose-like dermatitis due to trichloroethylene (OMDT) home and abroad, and the development of clinical practice and clinical related disciplines. The main modifications include: adding terms and definitions of OMDT, modifying the description of clinical manifestations of the diagnostic principles, adjusting the description of latency, deleting the diagnostic requirement of the incidence probability, adding the specific allergen patch test as the etiological diagnostic index, standardizing the application scope, operating procedure and precautions of the specific allergen patch test. In addition, the relevant content of “Basic Characteristics and Clinical Types of Skin Damage of Medicamentose-like Dermatitis due to Trichloroethylene” in Appendix A is improved, the treatment principles are revised, and the content of new progress in treatment, artificial liver application, are added. The revised GBZ 185-2024 Diagnostic Standard for Occupational Medicamentose-like Dermatitis due to Trichloroethylene is more scientific and practical, and can provide technical basis for the standardized diagnosis and treatment of OMDT in medical and health institutions.

OBJECTIVETo explore the role of prenatal exposure to lipopolysaccharides (LPS) on aortic morphology in the neonatal offspring rats.

METHODSTwelve pregnant rats were randomly divided into three groups: control group, LPS group, and PDTC (pyrrolidinedithiocarbamate, LPS+PDTC) group. The rats were intraperitoneally administered vehicle, LPS (0.79 mg/kg) , or LPS plus PDTC (100 mg/kg) , respectively. LPS was given on the 8th, 10th and 12th days, whereas vehicle and PDTC were given daily from the 8th to the 14th day during gestation. Histopathological alteration of the thoracic aorta was observed by hematoxylin-eosin staining and transmission electron microscopy, thoracic aortic mRNA and protein expression of connexin (Cx) molecules including Cx37, Cx40, Cx43 and Cx45 in offspring was detected by Real Time PCR and confocal laser-scanning microscope, respectively, offspring body weight was measure at day 1 and week 1.

RESULTSBody weight at 1 day and 1 week-old offspring was significantly lower in LPS group than in control group (P < 0.01), which were significantly higher in PDTC group compared to LPS group (P < 0.01): [1 day: control group (7.425 ± 0.146) g, LPS group (6.742 ± 0.128) g, PDTC group (7.137 ± 0.141) g; 1 week: control group (20.173 ± 3.982) g, LPS group (13.264 ± 2.581) g, PDTC group (17.863 ± 3.412) g]. In 1 week-old offspring of LPS group, the thoracic aortas exhibited lesions, including impaired endothelial cells, thickening and fibrous changes of intimae, and migration and proliferation of vascular smooth muscle cells; the number of gap junction was decreased versus control group and pathological changes were similar between PDTC group and LPS group. Cx43 protein expression in LPS group was obviously lower than in control group and which could be partly reversed in PDTC group. Expression of Cx43 mRNA was significantly lower in 1 day and 1 week offspring of LPS group compared to control group (P < 0.05), which could be reversed in PDTC group (P < 0.05) (1 day: control group 1.530 ± 0.296, LPS group 1.226 ± 0.209, PDTC group 1.619 ± 0.324; 1 week: control group 9.357 ± 1.917, LPS group 7.204 ± 1.165, PDTC group 9.271 ± 1.514).

CONCLUSIONOur results indicate that maternal LPS exposure during pregnancy leads to vascular changes in neonatal offspring which might increase the susceptibility to adult hypertension.

Animals ; Aorta, Thoracic ; drug effects ; pathology ; Connexin 43 ; metabolism ; Female ; Lipopolysaccharides ; toxicity ; Maternal Exposure ; Pregnancy ; Prenatal Exposure Delayed Effects ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate the association and interaction between smoking and the nicotine acetylcholine receptor subunits alpha 5(CHRNA5) gene polymorphisms on lung cancer in Chinese men.

METHODSA case-control study was employed with a total of 204 male lung cancer patients and 821 healthy control subjects enrolled in the study. All the subjects were interviewed under a structured questionnaire with the contents on socio-demographic status and smoking behavior. Venous blood samples were collected to measure single nucleotide polymorphism of rs17486278 in CHRNA5. A series of multivariate logistic regression models were performed to assess the association and interaction between smoking and the CHRNA5 gene polymorphisms on lung cancer.

RESULTSAfter controlling for potential confounding factors, data from the multivariate logistic regression analysis showed that individuals with smoking >15 cigarettes per day would significantly increase the risk of lung cancer when compared to the non-smokers (OR = 3.49, 95%CI:2.29-5.32). However, no associations between CHRNA5 rs17486278 polymorphisms and lung cancer were found. Furthermore, those who smoked 1-15 cigarettes per day had a positive interactive effect between rs17486278 CC genotype and lung cancer (OR = 16.13, 95% CI:1.27-205.33). Results from further stratified analysis on smoking behaviors and rs17486278 genotypes indicated that when compared with non-smokers on rs17486278 AA genotype, those individuals who smoked 1-15 cigarettes per day with rs17486278 CC genotype, individuals smoking >15 cigarettes per day with AA genotype and individuals smoking >15 cigarettes per day with AC genotype, all had a higher risk of developing lung cancer, with their OR value as 8.14(95% CI:1.17-56.56), 3.84 (95% CI:1.30-11.40) and 5.32 (95% CI:1.78-15.93), respectively.

CONCLUSIONThere was an interaction between smoking and CHRNA5 gene polymorphism on lung cancer.

Asian Continental Ancestry Group ; Case-Control Studies ; Genotype ; Humans ; Logistic Models ; Lung Neoplasms ; genetics ; Male ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide ; Receptors, Nicotinic ; genetics ; Risk ; Smoking