Objective To find a new method for quality control of polysaccharides by establishing the fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan. Methods The polysaccharides extracted from Guifu Dihuang Wan were hydro-lyzed partially under appropriate hydrolysis condition with acid,and the fingerprint profiles of the hydrolyzates was obtained by HPLC. Microsoft Excel 2002 was applied for calculating the similarity of fingerprint profiles,and informatton analysis of these profiles was performed by a software for evaluating fingerprint profiles of traditional Chinese medicine. Results Fingerprint profiles of the hydroly-zates of polysaccharides from 10 of Guifu Dihuang Wan products,produced by different manufacturers,were obtained. It was observed that the similarity of eight products was greater than 0.99,while the similarity of the other two was 0.92 and 0.94,respectively. Con-clusion This method is technically feasible for the quality control of polysaccharides from Guifu Dihuang Wan.

Objective To develop an HPLC method for the determination of ginsenoside Rb1 in Jupizhuru decoction,and de-termine the amount of ginsenoside Rb1 in 10 batches of Jupizhuru decoction composed of different batch herbs. Methods The HPLC analysis was carried out on a Phenomenex Luna C18 column with column temperature at 30℃,flow rate 1.0 ml/min,detection wave-length 203 nm and mobile phase acetonitrile and 0.1%phosphoric acid by gradient elution. Results The method was found to be lin-ear within the ranges of 5-500μg/ml(r=0.9999),and the average recovery of ginsenoside Rb1 was 95.5%(RSD 1.77%,n=6). Con-clusion The determination method is suitable for the quality control of ginsenoside Rb1 in Jupizhuru decoction.

OBJECTIVETo establish a method for content determination of polysaccharides in Periplocaforrestii.

METHODThe conversion coefficient of P. forrestii. polysaccharide to glucose was obtained by refined polysaccharides, and then the content of crude polysaccharides was determined by sulfuric-phenol method.

RESULTThe content of crude polysaccharides in P. forrestii. was 54.68% (RSD 3.0%, n = 6), and the average recovery was 101.7% (RSD 6.0%, n =6).

CONCLUSIONThe method was simple, rapid, and accurate.

Chromatography, High Pressure Liquid ; methods ; Colorimetry ; Drugs, Chinese Herbal ; chemistry ; Flowers ; chemistry ; Periploca ; chemistry ; Phenols ; analysis ; Polysaccharides ; analysis ; Spectroscopy, Fourier Transform Infrared ; methods

Tanshinone II A, which was known unique to the salvia, was separated and purified by silica gel column chromatography and recrystallisation from an ethyl acetate-soluble portion (the anti-inflammatory active portion) of ethanol extract of Periploca forrestii. The diterpenoid quinone was obtained from the Periploca for the first time.
Diterpenes ; analysis ; isolation & purification ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Periploca ; chemistry ; Quinones ; analysis ; isolation & purification