[Objective]To discuss the treatment to post-apoplexy depression with acupuncture of strengthening brain and regulating mind.[Method] Select 60 cases;randomly divide them into acupuncture group and western medicine group with 30 cases in each.Both accept basic therapy,the acupuncture group is added with acupuncture of strengthening brain and regulating mind,the other with fluxetine;for successive 4 weeks make scores to HRMD mete table,nerve function lack degree and daily life Barthel index.[Result] Both scores had changes after treatment,however the acupuncture group was better than another group,with marked cure effect,meanwhile with quantity-efficacy relationship.[Conclusion] Acupuncture of strengthening brain and regulating mind can relieve depression,promote nerve function recovery and improve life quality.

Congenital cataract is one of the leading causes of children vision impairment and blindness.Among the many causative factors,inheritance plays a critical role in the development of congenital cataract.With the development of molecular biology techniques,more than 30 genes have been found to be associated with congenital cataract,and the number is still increasing.More pathogenic genes discovery and functional researches have the important values to understand the lens biological characteristics and the cataract pathophysiology mechanism.Discovering new ways to prevent cataracts may make it possible for human to achieve individualized genetic intervention.This article reviewed the recent advances in the study of lens embryology and genes related to congenital cataract.

Personality vulnerability refers to some parts of personality,because of which an individual can not cope with pressure,thus violating individual's physical and mental health.The mechanism of disease triggered by personality vulnerability includes psychological-physiological and behavioral mechanisms.The problems in this field need to be further studied.

Currently the employment situation of graduates in China tend to be grim.This article attempts to describe several representative psycological problems faced by graduate students during job hunting and to analyze their possible causes.On this basis,the author proposes some educational countermeasures against such job-hunting psycological problems.

Objective Ovariectomized rats are the most suitable model for the study of postmenopausal osteoporosis.However,the effect of ovariectomy on rat skin is little studied.The aim of this study was to observe the effect of ovariectomy on the skin aging indexes in rats.Methods 20 healthy 3-month-old female SD rats were randomly divided into sham group and ovariectomized (OVX) group.Serum estradiol was detected at 3 months after ovariectomy,the skin aging-related biochemical indexes such as catalase (CAT),glutathione peroxidase (GSH-Px),superoxide dismutase (SOD) activity and the content of malondialdehyde (MDA),hydroxyproline (HYP) and lipofuscin (LF) were also detected.The structure of skin tissue was observed and detected using a pathological image analysis system.Results The serum E2 of OVX rats was significantly lower than that in the sham group,the thickness of epidermis and dermis of OVX group was significantly thinner than that of the sham group (P<0.01),the amount of collagen fibers was decreased and their arrangement was very loose.Compared with the sham group,the activity of CAT,GSH-Px and SOD in the OVX group was significantly decreased (P<0.01),and the content of MDA was significantly increased (P<0.01).The content of HYP in skin tissue was significantly decreased,and LF content was significantly increased (P< 0.01) in the OVX group.Conclusions Skin aging can be induced at 3 months after ovariectomy in 3-month old rats,which may be a good model for the study of skin aging in postmenopausal women.

Objective:There are many different kinds of biological safety cabinets, it make some difficulties in the allocation. Scientific selection which based experimental requirements choose the right equipment match experiments, it can effectively prevent the spread of pathogenic microorganisms of operation personnel damage and environmental pollution.Methods: According to its working characteristics, different laboratory levels, detection items and actual operation of dangerous levels of pathogenic microorganisms, to determine the matched biological safety cabinet.Results: According to the different experiments correctly, choose the appropriate biological safety cabinet, bio-safety has been obviously improved, the incidence of adverse events decreased from 12% to 3%; the experimenter's security has improved.Conclusion: Through the use of biological safety cabinet clear and selection method, can not only improve biological testing of safety consciousness, but also strengthen the biological safety standardization, institutionalization and standardization of the management mechanism, avoid the use of biological safety, improve the quality of the efficiency of laboratory work and experiment, laboratory personnel biological safety.

Ph-like acute lymphoblastic leukemia (Ph-like ALL) involves various genomic alterations associated with high risk factors and poor clinical outcome, such as IKZF1, CRLF2, JAK1/2, EBF1-PDGFRB, ATF7IP, EPOR, SH2B3.JAK/STAT pathway and PI3K/mTOR pathway are likely to be the targets of Ph-like ALL.Here,the above genomic alterations and treatment progress are reviewed.

Bronchial asthma is one of the most common chronic respiratory diseases in children.Airway remodeling that makes asthma difficult to control is an important pathological progress of asthma.Toll-like receptors (TLR), a kind of pattern recognition receptors, are expressed in a variety of human airway structure cells, such as airway epithelial cells, smooth muscle cells, mucus gland cells, etc.It is reported that TLRs play important roles in the regulation of airway remodeling in asthma.This article briefly summarizes the role of TLR in airway remodeling of asthma.

Mycoplasma pneumoniae (MP) is one of the most common agents of community-acquired pneumonia in children.MP has been reported in 10％ ～ 40％ of cases of community-acquired pneumonia and shows an even higher incidence during epidemics.MP infections display a spectrum of symptoms and signs,ranging from asymptomatic infection to severe and potentially fatal pneumonia or extrapulmonary manifestations.The pathogenesis of extrapulmonary manifestations remain largely unknown.This review classifies extrapulmonary manifestations due to MP infection into three categories:direct type,indirect type and vascular occlusion type.These manifestations include neurological,cardiac,hematological,dermatological and other symptoms.This article presents an overview of extrapulmonary manifestations and pathogenesis of MP infections in children to improve the comprehension for diagnosis.

Objective To investigate the resistance of imipenem resistant Pseudomonas aeruginosa (IRPA),metallo-β-lactamase (MBLs) producing and prevalences of the coding genes of IRPA in ICU.Methods Eighty-six clinical strains of Pseudomonas aeruginosa in ICU continuously collected were analyzed retrospectively.Pseudomonas aeruginosa and its drug susceptibility was identified by BioMerieux VITEK-32 system,and double disk synergy test was used to screen the MBLs producing IRPA.Then,the MBLs phenotype positive IRPA were confirmed by PCR to explore the genotype of them.Results Except polymyxin B with 100.0％ sensitivity and amikacin with higher sensitivity,the percentage of drug resistance for other antibiotics was more than 46％.MBLs phenotype positive IRPA accounted for 48.8％ (42/86) by double disk synergy test and genotype positive accounted for 47.7％(41/86) by PCR in MBLs phenotype positive IRPA.Among these,the most common was IMP-1 which constituted for 26.7％ (23/86).What's more,there were 15.1％(13/86) strains IRPA which carried multidrug resistant genes.Conclusions The main mechanism of drug resistance of IRPA in ICU is MBLs producing and IMP-1 is the most prevalent genotype,and IRPA with multidrug resistant genes is serious.Thus,monitoring of MBLs should be strengthened to help to use antibiotics rationally and prevent transmission of these genes.