Objective To investigate the effect of micro ecological preparation on imbalance of intestinal flora in pediatric blood cancer after chemotherapy.Methods The clinical data of 60 patients with blood cancer were retrospectively analyzed.All patients were divided into the observation group and control group by different trentment methods,30 cases in each group.The control group were received conventional chemotherapy,the observation group were given Lactobacillus triple viable Bifidobacterium piece(two piece once,tid,4 weeks for a course)on the basis of the control group,the intestinal flora(Enterobacteriaceae,Enterococcus,Bifidobacterium,Lactobacillus,Escherichia coli)were detected before and after chemotherapy,made a record of respiratory,urinary,digestive,skin infections after chemotherapy.Results The Bifidobacterium and Lactobacillus of the observation group after treatment were signifi-cantly higher than before treatment(t =3.947,3.636,all P <0.05),there were no difference in Enterobacter,Entero-coccus,Escherichia coli in the observation group before and after treatment(all P >0.05).The Enterobacter,Entero-coccus,Bifidobacterium,Lactobacillus,Escherichia coli of the control group were lower after treatment than before treatment(t =3.712,3.641,3.722,3.543,3.616,all P <0.05),the Enterobacter,Enterococcus,Bifidobacterium, Lactobacillus,Escherichia coli of the observation group after treatment were higher than that of the control group(t =3.327,3.641,6.378,7.367,3.364,all P <0.05).The respiratory tract infection,cholecystitis,colitis,urinary tract infections incidence of the observation group were 50.00%,6.67%,16.67%,23.33%,respectively,which were low-er than 93.33%,30.00%,63.33%,60.00% of the control group(χ2 =13.871,5.455,13.611,8.297,all P <0.05).Conclusion The intestinal flora may be caused for treatment of chemotherapy,which are the importmant rea-son of various infections after chemotherapy,the Probiotics can reconstruct the ecological balance of the gut,increase the number of probiotics,foster the growth of normal flora,adjust the intestinal flora,reduce the chance of infection, conducive to the successful completion of chemotherapy,which is worthy of clinical application.

Objective:To study the expression and significance of ADMA/NOS/NO/cGMP pathway in the maternal-fetal interface of patients with preeclampsia. Methods: 60 patients were selected and divided into severe preeclampsia group, mild preeclampsia group and gestational hypertensive group according to the disease condition, meanwhile 20 healthy pregnant women of singleton receiving cesarean section were chosen as control group. Levels of NO and cGMP in placenta of the four groups were detected and compared,and activities of total NOS in placenta were measured and compared. The expression of eNOS and iNOS in placental tissue was detected by immunohistochemistry SP method. High-performance liquid chromatography was used to detect the level of ADMA in HUVECs. Results:The levels of NO in placenta of severe and mild preclampsia groups were (7. 6±3. 6) and (11. 4±4. 3) μmol/g, which were statistically significantly lower than that in control group(P<0. 05). The levels of cGMP in placenta of severe and mild pre-clampsia groups were ( 3. 26 ± 0. 31 ) and ( 4. 53 ± 0. 42 ) pmol/g, which were aslo significantly lower than that in control group ( P<0. 05). In the four groups,the level of cGMP in placenta was positively correlated with the level of NO in placenta(r=0. 672). The activities of total NOS in placenta of severe and mild preeclampsia groups were (10. 4±3. 0)and (14. 8±1. 6)U/mg protein,which were statistically significantly lower than that in control group ( P<0. 05 ) . In placenta of the four groups, the activity of total NOS was positively correlated with the level of NO(r=0. 785). The expression of eNOS in placenta of severe and mild preeclampsia groups was statistically significantly lower than that in control group ( P<0. 05 ) , the expression of iNOS in severe preeclampsia group was significantly higher than that in the control group(P<0. 05). The levels of ADMA in HUVECs of severe preeclampsia group,mild pre-eclampsia group and gestational hypertensive group were statistically significantly higher than that in the control group(P<0. 05). The level of ADMA in HUVECs was negatively correlated with the level of NO in placenta of the four groups ( r=-0. 582 ) . Conclusion:ADMA/NOS/NO/cGMP pathway may play an important role in the pathogenesis of preeclampsia.

Objective To investigate the distribution of chronic disease and geriatric syndrome in hospitalized elderly patients with multimorbidity by chronic disease investigation and comprehensive geriatric assessment.Methods A total of 176 patients aged ≥60 years admitted into the geriatric department in our hospital were selected,and demographic factors were collected.Their chronic diseases and geriatric syndrome were recorded.The comprehensive geriatric assessment was consummated,and the biochemical indicators for chronic disease and geriatric syndrome were detected.Results All patients suffered from two or more kinds of chronic diseases.The top three most common diseases were hypertension (112 cases),diabetes (94 cases),and respiratory diseases (73cases).The sum of chronic diseases kinds were ≥3 in 137 patients,≥4 in 78 patients,≥5 in 40 patients.The amount of chronic diseases showed an increased tendency along with ageing.Totally 175 patients had more than one geriatric syndrome,the top three most common types were frailty and prefrailty(133 cases),visual impairment (117 cases),impaired daily activities (107 cases).There were significant differences in the number of geriatric syndrome among different age groups(x2 =16.989,P ＜ 0.001).Spearman analysis showed that the number of patients with geriatric syndrome were positively correlated with age(r =0.307,P＜0.001).Conclusions The prevalence of chronic disease and geriatric syndrome is high in hospitalized elderly patients with multimorbidity,which is increased with aging.We should pay attention to the screening of chronic disease and geriatric syndrome in multimorbid elderly patients