Objective To determine pathogens and epidemiological characteristics of adults with acute respiratory infection (ARI) in Beijing area.Methods During 2015,a total of 2 700 cases of ARI were sampled and detected for 9 respiratory pathogens including Legionella pneumophila type1 (LP1),mycoplasma pneumoniae (MP),qrickettsia (COX),chlamydia pneumoniae (CP),adenovirus (ADV),respiratory syncytial virus (RSV),influenza virus type A and B (INFA,IFVB) and parainfluenza viruses type1,2 and 3 (PIVs) using indirect fluorescence immunoassay.Results A total of 620 cases of ARI were tested positive with positive rate of 22.97％ (620/2 700).MP had the highest prevalence followed by LP1,INFB,COX,CP,PIVs,INFA,ADV and RSV in turn.There were 109 cases found mixed infection with the proportion of 17.58％ (109/620).Mixed infection of LP1 along with MP was the most common pattern.The highest detection rate of pathogens was observed in November,whereas the lowest in April in terms of months (x2 =31.288,P＜ 0.01).Different pathogens had distinct prevalent features.The prevalence of male was significant higher than that of female (x2 =6.402,P =0.011).As for stratificated by age,the middle-aged people had the highest infection rate (x2 =9.094,P=0.059).There was no significant difference between the out-and in-patient in terms of infection rate (x2 =0.114,P=0.736).Conclusion MP,LP1 and INFB accounted for ARI of adults in Beijing area during 2015.

Fifty five patients with alcohol induced-mental disorder (study group) and 43 local inhabitants without history of alcohol abuse (control group) were surveyed with family environment scale (FES-CV) and generic quality of life inventory-74 (GQOLI-74). The total score and the scores of all dimensions except material life in GQOLI-74 of study group were significantly lower than those of control group(P ＜0. 05). Compared with control group, the scores in FES of study group were lower for factors of cohesion, expressiveness, active-recreational orientation, moral-religious emphasis and organization in the patient's family, while the scores for conflict and control were higher( P ＜ 0. 05 or P ＜ 0. 01 ). The results indicate that family environment is closely correlated with quality of life in patients with alcohol-induced mental disorder, and family therapy would improve their quality of life.

Objective To analyze respiratory syncytial virus infection in infants and young children during 2014 and explore the relationship of infection status with the level of interleukin-4 in serum samples.Methods Totally 150 children with ARI were recruited in this study,and collected throat swabs from them.RSV was identified and differentiated into subgroups A and B by real-time RT-PCR.The total of IL-4 concentration in serum samples was measured by ELISA.Results Out of the 150 samples,32 (21.33％) were positive for RSV.Subtyping for A and B showed that 25 cases were subgroup A and 7 cases were subgroup B.Comparing between RSV infection group and normal control group,the former had higher serum IL-4 level [(328.67 ± 52.96) ng/L vs.(217.34 ± 51.21) ng/L,P ＜ 0.01],additionally B subgroup presented higher concentration than A subgroup [(371.09 ± 61.96)ng/L vs.(316.80 ± 44.63) ng/L,P ＜ 0.05].Analysis of clinical data indicated that infection of B subgroup probable caused more serious wheeze symptoms than that by subgroup A.Conclusions Infection of RSV subtype A was predominant in Oct.2014 to Mar.2015.Level of serum IL-4 was increased statistical significantly after RSV infection.Furthermore,infection of subtype B might cause more severe illness.

Objective:To investigate the mechanism of miR-218-5p regulating the glycolytic process in human non-small cell lung cancer A549 cells by targeting phosphodiesterase 7A(PDE7A).Methods:A549 cells were routinely cultured,and miR-218-5p mimic,mimic-NC,PDE7A overexpression plasmid(PDE7A-oe)and PDE7A control plasmid(PDE7A-NC)were transfected into A549 cells using Lipo3000,and recorded as the miR-218-5p mimic group,the mimic-NC group,the PDE7A-oe group and the PDE7A-NC group.The transfection efficiency was verified by qPCR assay;the expressions of glycolysis key enzyme proteins were detected by WB assay;the 2-deoxyglucose and lactate contents in A549 cells of each transfection group were detected by glucose assay and lactate production assay;the target binding relationship between miR-218-5p and PDE7A was verified by dual-luciferase reporter gene assay,and the data from the TCGA database were used to analyze the expression level of PDE7A mRNA in lung cancer tissues.Results:miR-218-5p was successfully overexpressed in A549 cells(P<0.01).Overexpression of miR-218-5p significantly inhibited the expressions of PDE7A,HK2,PKM2 proteins(all P<0.01),glucose uptake and lactate production(both P<0.01)in A549 cells.Overexpression of PDE7A significantly promoted the expressions of PDE7A,HK2,and PKM2 proteins(all P<0.01),as well as glucose uptake and lactate production(both P<0.01)in A549 cells.miR-218-5p in A549 cells could directly bind to the 3′-UTR of PDE7A mRNA.Database data analysis showed that PDE7A mRNA was highly expressed in lung squamous cell carcinoma tissues(P<0.01).Conclusion:miR-218-5p targets PDE7A to regulate the expression levels of HK2 and PKM2 in A549 cells,which in turn inhibits the glycolytic process.miR-218-5p/PDE7A may be a potential target for clinical diagnosis and treatment of NSCLC.

BACKGROUND: Cullin1 is a representative member of the Cullin family, and it plays an important role in the ubiquitination of cell cycle, transcription and signal transduction related proteins. Cullin1 is closely related to the occurrence and development of a variety of malignant tumors. The aim of this study is to investigate the effects of Cullin1 on biological function of lung adenocarcinoma A549 and H1395 Cells. METHODS: The expression of Cullin1 mRNA was detected by quantitative Real-time polymerase chain reaction in lung adenocarcinoma cells (A549, H358, H1395, H1650) and human normal lung epithelial cells BEAS-2B, siRNA technology was used to interfere with lung adenocarcinoma cells with relatively high expression of Cullin1 mRNA; cell proliferation, cell cycle distribution, early cell apoptosis, invasion and migration ability were detected by methyl thiazolyl tetrazolium assay (MTT), flow cytometry and Transwell experiment; Western blot was used to detect the expression levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), Cyclin D1, Cyclin E2, p21 and p27. RESULTS: Compared with the BEAS-2B cell, Cullin1 mRNA was highly expressed in lung adenocarcinoma cells, especially in lung adenocarcinoma A549 and H1395 cells (P<0.05). The proliferation ability of lung adenocarcinoma cells was inhibited after interference with Cullin1, and the number of cells in G1 phase increased, the number of cells in S phase decreased, and the early apoptosis rate of lung adenocarcinoma cells is significantly increased (P<0.05); The invasion and migration ability of lung adenocarcinoma cells decreased (P<0.05). After interference with Cullin1, the protein expression of MMP-9, MMP-2, CyclinD1 and CyclinE2 decreased (P<0.05), while the expression of TIMP-1, p21 and p27 protein increased (P<0.05). CONCLUSIONS Interference with Cullin1 inhibits the proliferation, invasion and migration of lung adenocarcinoma A549 and H1395 cells, Cullin1 plays a role in promoting cancer in lung adenocarcinoma.