Objective To study the role of focal adhesion kinase in the breast carcinoma cell adhesion and migration mediated by coagulation factor Ⅶa combined with tissue factor to explore the mechanism of tissue factor promoting metastasis. Methods The count of adherent cells were detected by using colorimetric assay,cell migration was detected with modified Bodyen chamber,and the FAK and the tyrosine phosphorylation of FAK were detected by immunoprecipitation and Western blot.Results Factor Ⅶa combinaed with tissue factor induced the tyrosine phosphorylation of FAK,which took part in the cancer cell's migration.Conclusion FAK is an important message of the breast carcinoma's adhesion and migration mediated by TF.

Objective To study the rule of Apaf-1 in wnt/β-catenin signaling pathway and its regulatory expression mechanism in hepatocellular carcinoma cells.Methods The regulatory mechanism of Apaf-1 gene in Wnt/β-catenin signaling pathway was verified by the TOPflash experiment;real-time PCR was used to detect the expression amounts of Apaf-1 in various hepatoma cell lines (HepG2,H HCC,HB611) and normal liver cell line(LO2);the RNAi interference plasmid of Apaf-1 was constructed and transfected into the HepG2 cell line.Then the transfection efficiency and expression of related genes and proteins were detected.Results The TOPFlash experiment found that Apaf-1 gene could inhibit the wnt/β-catenin signal pathway in a dose dependent manner;the Apaf-1 expression level in hepatoma cells was decreased compared with the normal liver cells,moreover its expression level was lowest in HepG2 cell line;RNA interfering Apaf-1 gene in HepG2 cell line,the expression level of Apaf-1 gene was significantly decreased,and the expression levels of downstream genes and protein(β-catenin,Cyclin A,CDK2,wnt5a,STAT3,EGFR,APC) in wnt signal pathway were significantly increased or decreased,the difference was statistically significant(P＜0.05).Conclusion Apaf-1 gene plays an important role in the formation process of hepatocarcinoma cells by wnt/β-catenin signaling pathway.

For the defects of interpreting the relationship between the distribution and severity of vascular stenostic disease and the low shear stress, the study focus on and explore the rules of the pressure distribution and variation downstream of the stenostic vessel with the numerical simulation and the hydrodynamic model of turbulent flow separation area for in vitro experiment. The results reveal that there is a good compatibility between the model experiment and the numerical simulation; there exists a marked pressure decrease downstream of the stenosis. And the distribution area of low pressure is similar to the characteristic distribution of the stenostic vessel diseases. The study suggested that the numerical simulation can be applied in the study of micro-flow field; the presure distribution and variatioin downstream of the stenostic vessel maybe another factor to trigger the pathogenesis of vessel stenostic diseases.
Blood Flow Velocity ; physiology ; Blood Pressure ; physiology ; Constriction, Pathologic ; physiopathology ; Humans ; Models, Biological ; Vascular Diseases ; physiopathology

Focus on the defects of the extraction method of velocity pulsated signals in the velocity spectrum image of pulsed Doppler ultrasounds (such as the limited data, great amount of work and low reproducibility), we combine the earlier research results and the basic rules of the velocity spectrum image, and employing the visual basics plateau, to design and develop a software to extract the velocity signals from the spectrum image automatically. Compare the mean velocity and RNS between the PIV and the PDU resulting from the method, the correlations are r = 0.93 and r = 0.78 respectively. The study reveals the software increase the amount of valid data, decrease the amount of work and increase reproducibility of the signals extraction. The method may be an effective way to analyse the velocity spectrum image of PDU in clinic.
Arteries ; diagnostic imaging ; Blood Flow Velocity ; physiology ; Humans ; Pulsatile Flow ; Signal Processing, Computer-Assisted ; Software ; Ultrasonography, Doppler, Pulsed ; methods

This paper reports the establishment of a model of turbulent flow separation area for experiment on the downstream of tubal stenosis, and adjust it to cooperate with the velocity and turbulent shear stress (TSS) detection by means of the particle image velocimetry (PIV), and with the pressure detection of pressure sensor in vitro. The velocity, TSS and wall pressure characteristics of the downstream of tubal stenosis were quantitatively detected and analyzed via the PIV and pressure sensor. And the hydrodynamic characteristics of the velocity, TSS and wall pressure in the flow separation area were primarily understood. The model can cooperate smoothly with the PIV and pressure sensor to detect the velocity, TSS and wall pressure; there exist low velocity, low TSS and low pressure in the flow separation area downstream of tubal stenosis.
Blood Flow Velocity ; physiology ; Computer Simulation ; Constriction, Pathologic ; physiopathology ; Heart Valve Diseases ; physiopathology ; Models, Cardiovascular ; Rheology ; Stress, Mechanical ; Vascular Diseases ; physiopathology

OBJECTIVETo investigate the effect of environmental risk factors on the development of stroke.

METHODSWith the use of cold-stimuli plus high-salt intake as environmental risk factors, a hypertension model with the complication of stroke was established in rats, then, a new technique, suppression subtractive hybridization (SSH), was used to identify the differential genes which specifically expressed in total cerebrum tissue of rat in each group. Comparison was made between control group and stroke group.

RESULTSBy the application of SSH, a total of 576 clones were generated in this study from two subtractive libraries, among them 456 clones were usable and were analyzed. Genes for cell/organs defense were down-regulated in stroke group and metabolism transcripts were shown to be up-regulated (P<0.01).

CONCLUSIONCell/organs defense genes may play important roles in the development of stroke. The above findings suggested that environmental risk factors could genetically alter individual sensitivity to stroke.

Animals ; Brain ; metabolism ; pathology ; Cell Division ; genetics ; Cold Temperature ; DNA, Complementary ; chemistry ; genetics ; Expressed Sequence Tags ; Gene Expression Regulation ; drug effects ; Immunity, Innate ; genetics ; Male ; RNA, Messenger ; drug effects ; genetics ; metabolism ; Rats ; Rats, Wistar ; Sequence Analysis, DNA ; Signal Transduction ; genetics ; Sodium Chloride, Dietary ; administration & dosage ; Stroke ; genetics