Objective To investigate the growth of rabbit osteoblast on the composite bioactive glass material scaffolds and to explore the experimental methods of optimized material scaffolds in bone tissue engineering. Methods The mesenchymal stem cells(MSCs) were separated and cultured from rabbit thigh marrow,and they were induced and differentiated into osteoblast by the revulsant.The change of typical appearance of the MSCs was investigated under microscope.The cytological characteristics of the MSCs were observed through cells activity and immunohistochemistry method.The osteoblast was cocultured with three various bioactive glasses respectively.The compatibility between the various bioactive glasses and osteoblast was compared by observation of the changes of the cells.Results The MSCs were successfully induced and cultured in the presence of the osteoblast revulsant.After inducement,these cells displayed osteoblast-like morphology.The bioactive glass composite scaffolds supported the attachment of cultured rabbit osteoblast.These cells proliferated faster on scaffolds with higher poriness of 90.20% and 94.50% than with lower poriness of 75.90%.Conclusion It is feasible to use bioactive glass composite scaffolds with proper poriness for bone tissue engineering.

The peripheral tissues mainly clear the metabolites through lymphatic system to maintain the homeostasis.In recent years,it has been found that there is a corresponding structure in the brain,namely the glymphatic system,which consists of multiple components,including the para-arterial influx of fluids,the convective bulk of interstitial fluid flow facilitated by aquaporin 4 and the paravenous efflux of fluids;the glymphatic system plays an important role in the removal of metabolites.This article will describe its origin,composition,driving mechanisms and influencing factors,and introduce its corresponding role in the pathological process of brain diseases such as Alzheimer's disease,Parkinson's disease and hydrocephalus.