Objective: To analyze the distribution and associated factors of high-risk genotypes of HPV in cervical infection among women in Shenzhen. Methods: The information on sociodemographic characteristics and HPV genotypes of HPV-positive women who participated cervical screening test from January 2014 to December 2016 was downloaded from Shenzhen Maternity and Child Healthcare Management Information System. According to the pathogenicity, the high-risk HPV genotypes were divided into 15 types including HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68; and there were 6 low-risk genotypes including HPV 6, 11, 42, 43, 44, and 81. Chi-square tests were applied to compare the proportions of high-risk HPV infection among women who had different sociodemographic characteristics. A non-conditional logistic regression model was used to analyze the associated factors for high-risk HPV infection. Results: In total, all HIV positives received HPV genotyping, with an average age of (38.08±9.38) years old. There were 9 979 (93.9%) high-risk and 645 (6.1%) low-risk HPV infections. The proportions of HPV infections for high-risk type in each year were 91.5%, 93.8%, and 95.6%, increasing with the screening years (χ²=54.79, P<0.001). Multivariate logistic regression analysis showed that compared with women younger than 25 years old, women in other age groups (at age 26 to 30 years, 31 to 35 years, 36 to 40 years, 41 to 45 years, and 50 years or older) had increased risks of high-risk HPV infection, with OR (95%CI) of 1.67 (1.20-2.31), 1.49 (1.09-2.03), 1.71 (1.23-2.37), 1.65 (1.19-2.31), and 1.84 (1.26-2.67), respectively; compared with the married, single women had a decreased risk of high-risk HPV infection (OR (95%CI): 0.71 (0.50-1.00)); women received HPV testing in 2015 and 2016 showed higher risk of high-risk HPV infection than those in 2014 (OR (95%CI): 1.43 (1.17-1.74) and 2.03 (1.68-2.46)). The 5 most common HPV genotypes were HPV52 (25.1%, 2 670 cases), followed by HPV16 (19.2%, 2 041 cases), HPV58 (13.3%, 1 413 cases), HPV18 (9.9%, 1 048 cases), and HPV51 (9.3%, 993 cases). Conclusion Age, marital status, and screening year were associated with high-risk HPV infections. Besides HPV16 and HPV18, the prevention and control on HPV infections for HPV52, HPV58, and HPV51 should be prioritized in Shenzhen area.

Objective To investigate the correlation between plasma miR-497-5p and miR-331-5p levels and the severity of neurological deficit and incidence of post-stroke depression(PSD)in pa-tients with ischemic stroke(IS).Methods A total of 191 IS patients attended to the First Affilia-ted Hospital of Nanyang Medical College from July 2021 to July 2023 were enrolled as the study group,and another 191 healthy individuals who taking physical examination in the same period were recruited as the control group.According to the NIHSS score,the patients were divided into mild,moderate and severe neurological deficit subgroups(82,68 and 41 cases,respectively),while according to the results of Hamilton Depression Scale,112 cases were assigned into a non-PSD subgroup and 79 cases into a PSD subgroup.Pearson and Spearman correlation analyses were used to analyze the correlation between plasma miR-497-5p and miR-331-5p levels and NIHSS score.Multivariate logistic regression analysis was employed to identify the risk factors for the occur-rence of PSD in IS patients,and ROC curve was plotted to evaluate the predictive values of plasma miR-497-5p and miR-331-5p levels for the occurrence of PSD in IS patients.Results The plasma miR-497-5p and miR-331-5p levels were significantly higher in the study group than the control group(P＜0.01).The levels and NIHSS score were significantly increase in the mild,moderate and severe neurological deficit subgroups in order(P＜0.01).The two levels and NIHSS score were all positively correlated(P＜0.01).The PSD group had more severe deficiency,and higher levels of miR-497-5p and miR-331-5p than the non-PSD group(P＜0.01).Multivariate logistic regression analysis showed,the plasma miR-497-5p and miR-331-5p levels and severe deficiency were risk factors for the occurrence of PSD in IS patients(OR=3.248,95％CI:1.623-6.501;OR=4.258,95％CI:1.739-10.428;OR=2.261,95％CI:1.261-4.055).The AUC value of miR-497-5p,miR-331-5p and their combined together for predicting the occurrence of PSD in IS patients was 0.836,0.817 and 0.911,respectively,with the value of combined indicators was supe-rior to that of single one(P＜0.05).Conclusion Plasma miR-497-5p and miR-331-5p levels are significantly elevated in IS patients,which are closely associated with the severity of neurological deficits and the occurrence of PSD.Their combination shows a good predictive value for the occur-rence of PSD in IS patients.

Objective: To analyze the epidemiological genotype features of human papillomavirus (HPV) in cervical infection and their risks for cervical precancers among women in Shenzhen area. Methods: A total of 2 717 individuals ranging in age from 30~59 years were recruited in 18 community health centers of Shenzhen city from March 1 to June 15, 2015 by a cluster sampling method. The results of genotype of HPV, liquid-based cytology (LBC), colposcopy and pathology were analyzed. The clinical sensitivity and specificity as well as positive (PPV) and negative (NPV) predictive values of the combination of different HPV genotype in screening the cervical intraepithelial neoplasia (CIN) 2 and above were estimated. Results: The HPV infection rate in Shenzhen area was 15.9% (432/2 717). The most common HPV genotype was HPV52 (22.9%), followed by HPV16 (12.7%), HPV53 (10.0%), HPV51 (8.6%) and HPV58 (8.1%). Compared with HPV16/18 genotyping, HPV33/16 genotyping had a higher sensitivity (57.1% vs. 42.9%, P<0.05) and an analogous specificity (87.3% vs. 86.9%, P>0.05) in predicting CIN2+ . The sensitivity of combination of HPV33/16 genotyping and low grade squamous intraepithelial lesion (LSIL) positive tested by LBC in predicting CIN2+ was 75.0%, significantly higher than 64.3% of atypical squamous cells of undetermined significance (ASC-US) positive tested by LBC alone (P<0.05). The specificities of these two methods mentioned above in predicting CIN2+ were 83.5% and 89.2%, respectively, without statistical difference (P>0.05). Conclusions Women infected by HPV have distinct risks for CIN2+ according to different high-risk HPV genotypes. The top five risks were HPV 33, 16, 58, 56, and 68. HPV-positive women triaged by LBC LSIL+ combined with HPV33/16 genotyping may be a potential strategy for cervical cancer screening in developed urban area.