1.Relationship between Electroencephalogram in Preoperative Evaluation and Postoperative Seizures Ending: 168 Cases Report
Yuan ZHAO ; Shangmeng HUANG ; Chengyun DING
Chinese Journal of Rehabilitation Theory and Practice 2013;19(2):180-182
Objective To retrospectively conclude the characteristics of interictal EEG of patients with intractable epilepsy, and then analyze their relationship with postoperative seizures. Methods 168 patients with intractable epilepsy in the epilepsy center of Beijing Tiantan Hospital that underwent strictly preoperative evaluations before surgery were collected and followed up for 2 and 5 years, the characteristics of their interictal EEG and postoperative seizures after 2 years and 5 years were concluded and analyzed. Results There was no significant difference between 2 years and 5 years postoperative seizures in 73 intractable epilepsy patients (P>0.05). There was statistically differences in postoperative seizures between unilateral and bilateral abnormal discharges (P<0.05). Conclusion Patients with unilateral abnormal discharges and located at temporal in interictal EEG predict better outcomes 2 years after operation.
3.GLP-1 agonist protects myocardial hypoxia-reoxygenation injury through PI3K/ Akt signaling pathway: an experimental study
Zhigang YI ; Jinhan CHEN ; Jin LI ; Wenan GUO ; Juan WU ; Shangmeng HUANG
Chinese Journal of Endocrinology and Metabolism 2018;34(1):61-66
Objective To investigate the effects of glucagon like peptide 1 (GLP-1) agonist on myocardial hypoxia reoxygenation injury and its molecular mechanism. Methods H9C2 cells were divided into control group, hypoxia reoxygenation(H/ R) group, H/ R+GLP-1 group, and H/ R+GLP-1+phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 group. The cell proliferation activity, apoptosis rate, enzyme contents in the medium and the expressions of apoptosis-related genes were detected. After animal model of myocardial ischemia reperfusion injury (I/ R) was established and was treated with GLP-1 agonist and PI3K inhibitor, serum enzyme contents were detected. Results Hypoxia reoxygenation decreased the myocardial cell proliferation activity and phosphorylated-PI3K(p-PI3K), phosphorylated-protein kinase B (p-Akt), Bcl-2 protein expressions, increased the apoptotic cell number and creatine kinase ( CK), creatine kinase-MB ( CK-MB), lactate dehydrogenase ( LDH) contents in cell culture medium and Bax, caspase-3 protein expressions, which were ameliorated by GLP-1 ( all P < 0. 05). The myocardial cell proliferation activity and Bcl-2 protein expression of H/ R+GLP-1+LY294002 group were significantly lower than those of H/ R+GLP-1 group while the apoptotic cell number and CK, CK-MB, LDH contents in cell culture medium and Bax, Caspase-3 protein expressions were significantly higher (all P<0. 05). Serum CK, CK-MB, and LDH contents in rats of I/ R group were significantly higher than those in control group and I/ R+GLP-1 group. Serum CK, CK-MB, and LDH contents in rats of I/ R+GLP-1+LY294002 group were significantly higher than those in I/ R+GLP-1 group(all P < 0. 05). Conclusion GLP-1 agonist is able to protect the myocardial hypoxia reoxygenation injury via activating PI3K/ Akt signaling pathway.