Objective To investigate the influent factors on normal intraabdominal pressure (IAP) and establish the IAP regressive equation. Methods The IAPs of 106 hospitalized patients were determined through monitoring the bladder pressures. The relationship between IAP and 14 factors including age, gender, height, weight, body mass index (BMI), previous and recent abdominal surgical history, reasons for admission,complications and their quantities were analyzed,respectively Results The mean IAP of the hospitalized patients was 5.5 mm Hg with a range from 0.4 mm Hg to 12.8 mm Hg. The difference among IAPs of different grades of BMIs had statistical significance ( F =5.550, P 0.05). Conclusion Normal IAP is possibly influenced by gender and BMI,and it is individually different.

Objective To investigate the protective effect of Klotho on renal ischema-reperfusion injury (IRI).Methods Plasmid expression vector of secreting type Klotho (pV5-sKlotho) was injected through the tail vein 24 h before operation.The renal ischemia-reperfusion model was established and the experiment was divided into 6 groups:sham group of young mice,sham group of aged mice,control group of young mice,control group of aged mice group,Klotho transfected group of young mice and Klotho transfected group of aged mice.The serum creatinine (Cr) and blood urea nitrogen (BUN) levels were determined at 1st,3rd,7th,and 14th after reperfusion.The mRNA expression of Klotho and P53 in the renal tissue,concentration of the malondialdehyde (MDA),superoxide dismutase (SOD) and pathological changes in the renal tissue were examined.Results As compared with young mice group,the Cr and BUN levels were significantly increased after reperfusion (P<0.05),the expression of Klotho mRNA was significantly decreased (P<0.05) and the expression of P53 mRNA was significantly increased (P<0.05),the MDA level in the renal kidney tissue was significantly increased and SOD level was significantly decreased (P<0.05) in aged mice.Vacuolar degeneration and necrosis in tubules were major pathological changes 24 h after operation in aged mice,and at 14th day after IRI renal tubular atrophy,fibrosis and inflammatory cell infiltration were seen in aged mice.After transfection with pV5-sKlotho,as compared with control group of aged mice,the kidney function was significantly improved in Klotho transfected group of aged mice (P<0.05),and the up-regulated expression of Klotho and down-regulated expression of P53 were detected in Klotho transfected group of aged mice (P<0.05),also the MDA level in the renal tissue was obviously reduced and SOD level was obviously increased (P<0.05).Vacuolar degeneration and necrosis in tubules at 24th h after IRI and tubular atrophy,fibrosis and inflammatory cell infiltration at 14th day after IRI were significantly improved in Klotho transfected group of aged mice.Conclusion Transfection with Klotho can protect the acute kidney injury and chronic kidney disease induced by IRI in aged mice.

Objective To explore the effect of back Tuina on motor behavior,oxidative stress and in-flammation in rats with chronic fatigue syndrome(CFS).Methods Twenty-four Sprague-Dawley rats were randomly divided into a blank group,a model group and a Tuina group,each of 8,according to a random number table.The CFS rat model was prepared by means of forced weight-bearing swimming combined with chronic stress stimulation in 21 days.After modeling,the Tuina group was given daily 20-minute Tuina for 14 days.The general condition semi-quantitative score,exhaustion swimming time and open field experiment(OFE)distance of all groups were recorded.After the experiment,sam-ples were collected,and the histopathological changes of the vertical spine muscles were observed by hematoxylin and eosin(HE)staining.The cross-sectional area and diameter of muscle fibers were calcu-lated using Image Pro Plus software,and the frequency distribution diagram of cross-sectional area of muscle fibers was processed by using the Origin software.The contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and peroxisome proliferator-activated receptor γ-coactivator 1α(PGC-1α)and tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6 in the serum of rats were mea-sured.Results After the intervention,the general condition semi-quantitative score of the Tuina group was significantly lower than the model group(P<0.01),while the exhaustion swimming time and OFE distance were significantly higher than the latter group(P<0.05,P<0.01).(2)HE staining showed that the significant atrophy of erector spinal muscle cells in the model group,was significantly relieved in the Tuina group.(3)Compared with the blank group,the contents of SOD,GSH-Px and PGC-1α in erectus muscles decreased significantly(P<0.01),while those of TNF-α,IL-1β and IL-6 in the se-rum of the model group increased significantly(P<0.01).However,compared with model group,the contents of SOD,GSH-Px and PGC-1α in erectus muscle increased significantly(P<0.05,P<0.01),while those of TNF-α,IL-1β and IL-6 of the Tuina group decreased significantly(P<0.05,P<0.01).Conclusion Tuina in the back can regulate the oxidative stress response,reliever the inflammatory re-sponse and improve the motor behavior of CFS rats.