Pulse-taking is the most specific diagnostic method of TCM.Its significance has been recognized by ancient and modern doctors.However,at present the application and spread of pulse-taking is limited.In this paper,the author made detailed explorations of literatures and studies the value of pulse condition in guiding syndrome differentiation of liver diseases.The study shows abnormal pulse in left guan and taut pulse in cunkou location both suggest the liver is disordered.We can judge the location of diseases through pulse condition.This exploration shows pulse-taking plays important role in syndrome differentiation.But there is one thing should be paid more attention,pulse condition should be integrated with other symptoms,that will be reliable in guiding diagnosis.

OBJECTIVE: To study the correlation between the stromal cell-derived factor (SDF-1) and the receptor fusin (CXCR4) in carcinoma of larynx, and investigate some mechanisms of SDF-1/CXCR4 during the development, invasion and lymph node metastasis of laryngocarcinoma. METHOD: Detecting the expression of SDF-1 and CXCR4 by immunohistochemical method (SP) in laryngocarcinoma, paraneoplastic tissues, normal laryngeal mucosa and cervical lymph node. Using Kruskal-Wallis H test, chi2 test, Spearman rank correlation analysis and so on to do statistical analysis. RESULT: The positive expression rate of SDF-1 and CXCR4 in laryngocarcinoma was obviously higher than in paraneoplastic tissues and normal laryngeal mucosa tissues (P < 0.01). And the expression of two proteins was correlated with lymph node metastasis (P < 0.01), clinical stage (P < 0.01) and pathological grading of tumor (P < 0.05). The positive expression rate of SDF-1 and CXCR4 protein in metastasis lymph node tissue was higher than that in non metastasis lymph node tissue (P < 0.01). The expression of SDF-1 is correlated positively with the expression of CXCR4 in laryngocarcinoma. CONCLUSION SDF-1 and CXCR4 protein are highly expressed in laryngocarcinoma and in metastasis lymph node tissue. And they are correlated with lymph node metastasis, clinical stage and pathological grading of the tumor. According to the results, the two proteins may relate to infiltration and metastasis of laryngeal squamous cell carcinoma and play a role of synergistic action in the development and invasion of carcinoma of larynx.
Adult ; Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Chemokine CXCL12 ; metabolism ; Female ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; Lymph Nodes ; metabolism ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Receptors, CXCR4 ; metabolism

Objective To systematically evaluate the efficacy and safety of decompressive craniectomy and conservative treatment within 48 h of onset in malignant middle cerebral artery infarction.Methods Cochrane Library,Pubmed,Embase,CNKI,Chinese Biomedical Database,VIP information database,Wanfang database were searched,and the retrieval time was from the library being built to April 31,2015.Review Mannager 5.2 statistical analysis software was used to evaluate the treatment efficacy of decompressive craniectomy and conservative therapy,amd modified Rankin scale (mRS) scores were considered as the efficacy evaluation criteria.Results A total of six randomized controlled trial studies and two prospective studies were selected,including 201 patients from the decompressive craniectomy group and 197 patients from the conservative treatment group.The mortality of the two groups atter 12 months of treatment was significantly different (mRS scores=6,P=-0.000,OR=0.18,95％ CI:0.12-0.29).Moderate or severe disability rate after 6 months of treatment was significantly different (mRS scores=4-5,P=0.000,OR=3.36,95％CI:1.95-5.78),and that after 12 months of treatment was also significantly different (P=0.000,OR=4.62,95％CI:2.64-8.07).The number of patients enjoyed good life quality (mRS scores ≤ 3) 6 and 12 months after treatment between the two groups was statistically significant (P=0.010,OR=2.69,95％CI:1.21-5.96;P=0.020,OR=2.07,95％CI:1.11-3.87);mortality rate (mRS scores=6) and disability rate (mRS scores=3-5) of patients aged more than 60 years between the two groups were significantly different (P=0.000,OR=0.20,95％CI:0.10-0.42;P=0.000,OR=4.94,95％CI:2.35-10.35).Conclusion Regardless of age greater or less than 60 years old,decompressive craniectomy can significantly reduce the mortality of patients with malignant middle cerebral artery infarction within 48 hours as compared with conservative treatment,but surgery may increase moderate to severe disability.

Objective:To analyze the gene mutation types and haematological characteristics of αβ compound thalassemia, non-delectable α-thalassemia and Hemoglobin H Disease (HbH disease) in Foshan.Methods:Using the method of retrospective analysis, we selected the population who had been tested for thalassemia gene in Foshan Second People's Hospital Affiliated to Southern Medical University from January 2011 to November 2019. Sysmex XT-5000 automatic hematology analyzer was used for routine blood analysis. α-, β- thalassemia genes were detected by PCR + diversion hybridization method.Results:A total of 4 563 people were tested, of which 1 829 were diagnosed as thalassaemia through genetic diagnosis. αβ compound thalassaemia was detected in 81 cases with a positive rate of 1.8%; non-delectable α-thalassemia was detected in 18 cases with a positive rate of 0.4%; HbH disease was detected in 23 cases with a positive rate of 0.5%. The most common genotypes of αβ compound thalassemia were -- SEA/αα\β41-42/βN (17.3%, 14/81), -α 3.7/αα\β41-42/βN (14.8%, 12/81), -- SEA/αα\β654/βN (11.1%, 9/81). The main manifestations of hematology were normal to mild anemia (93.8%, 76/81). Only β-thalassemia with double heterozygotes and α-thalassemia showed severe anemia. αα CS/αα\βN/βN genotypes were common in the local non delectable α-thalassemia (50.0%, 9/18), and the non delectable α-thalassemia was characterized by non-positive phenotype or typical small-cell hypochromatosis in hematology. The genotypes of local HbH patients were -α 3.7/-- SEA\βN/βN (65.2%, 15/23), and simple HbH manifested as moderate anemia (87.0%, 20/23). Patients with HbH disease and β-thalassemia had normal or mild anemia (13.0%, 3/23). Conclusions:The genotypes of αβ compound thalassemia in Foshan area are diverse and complex, and hematology mainly manifests as mild anemia or normal. Non-delectable α-thalassaemia is common in the genotype of αα CS/αα\βN/βN, and clinical manifestations are asymptomatic gene carriers. The genotype of local HbH patients is mainly -α 3.7/-- SEA\βN/βN, and the hematology mainly shows moderate anemia.

Objective To evaluate the diagnostic value of plasma the soluble CD14 subtype(sCD14-ST,renamed as presepsin)in early diagnosis of sepsis patients with acute kidney injury(AKI).Methods A total of 110 patients with AKI in intensive care unit(ICU)of Wuhu Hospital Af-filiated to East China Normal University from January 2021 to December 2022 were prospectively se-lected.The patients were divided into AKI stage 1 group(n=34),AKI stage 2 group(n=36)and AKI stage 3 group(n=40)according to the staging criteria defined byKidney Disease:Improving Global Outcomes(KDIGO)in 2012,and 53 non-AKI patients in the same period were selected as the control group.The patients were further divided into non-sepsis group and sepsis group according to whether combined sepsis or not.Plasma Presepsin levels were recorded in all the patients.Receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of Presepsin inearly sepsis in AKI patients,and the optimal cutoff value was found using Jorden index.Results The level of Presepsin in the sepsis group was higher than that in non-sepsis group(P＜0.05).In non-sepsis patients,the level of Presepsin increased gradually with the aggravation of renal function injury(P＜0.05).The level of Presepsin in stage 3 AKI group was higher than that in non-AKI group(P＜0.01).ROC curve analysis results showed that thearea under the curve(AUC)of Presepsin level for diagnosing sepsis in stage 1 AKI group,stage 2 AKI group and stage 3 AKI group was smaller than that in non-AKI group(P＞0.05).Conclusion Presepsin can be used as a marker for early diagnosis of sepsis in AKI patients,but different thresholds should be used according to the severity of renal impairment.

Objective To evaluate the diagnostic value of plasma the soluble CD14 subtype(sCD14-ST,renamed as presepsin)in early diagnosis of sepsis patients with acute kidney injury(AKI).Methods A total of 110 patients with AKI in intensive care unit(ICU)of Wuhu Hospital Af-filiated to East China Normal University from January 2021 to December 2022 were prospectively se-lected.The patients were divided into AKI stage 1 group(n=34),AKI stage 2 group(n=36)and AKI stage 3 group(n=40)according to the staging criteria defined byKidney Disease:Improving Global Outcomes(KDIGO)in 2012,and 53 non-AKI patients in the same period were selected as the control group.The patients were further divided into non-sepsis group and sepsis group according to whether combined sepsis or not.Plasma Presepsin levels were recorded in all the patients.Receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of Presepsin inearly sepsis in AKI patients,and the optimal cutoff value was found using Jorden index.Results The level of Presepsin in the sepsis group was higher than that in non-sepsis group(P＜0.05).In non-sepsis patients,the level of Presepsin increased gradually with the aggravation of renal function injury(P＜0.05).The level of Presepsin in stage 3 AKI group was higher than that in non-AKI group(P＜0.01).ROC curve analysis results showed that thearea under the curve(AUC)of Presepsin level for diagnosing sepsis in stage 1 AKI group,stage 2 AKI group and stage 3 AKI group was smaller than that in non-AKI group(P＞0.05).Conclusion Presepsin can be used as a marker for early diagnosis of sepsis in AKI patients,but different thresholds should be used according to the severity of renal impairment.