Objective To explore the curative effect of local oxygen therapy in combination with external application of Houfengsan and vitamin AD mixture in the treatment of red breech. Methods 100children with red breech were divided randomly into the treatment group and the control group with 50 cases in each group. The treatment group was treated with direct local oxygen blowing for 10～15 minutes and applying mixture of Houfengsan and vitamin AD, the control group was treated with 3% zinc oxide unguent.The treatment effect and cure time were compared between the two groups. Results The total efficient rate of the treatment group was 100% ,which was higher than that of the control group. The cure time of the treatment group was (3.92±0.98) days ,and (6.66t1.46) days in the control group, and the difference was significant between the two groups. Conclusions Local oxygen blowing with external application of mixture of Houfengsan and vitamin AD is easy to accept by children, safe, economical and simple to operate. It is an effective method to treat red breech.

Objective To discuss the coping methods of pediatric nurses with adverse psychology and behavior of parents with terminal children. Methods 198 parents who were assessed with adverse psychology and behavior with terminal children were chosen and randomly divided into the observation group(100 cases) and the control group(98 cases). The control group was given conventional care, while in the observation group, nursing intervention of humanistic care and reinforced health education were adopted in addition. The changes of adverse psychology and behavior of the two groups were observed. Χ2 test was used. Results The adverse psychology and behavior in the observation group was reduced significantly than that of the control group after nursing intervention. Conclusions Nursing intervention can effec-tively mitigate the adverse psychology and behavior in parents with terminal children. It plays an active role beth on the terminal children and their parents.

OBJECTIVES: The previously established 38-plex InDel system was optimized and its performance was validated according to the Scientific Working Group on DNA Analysis Method (SWGDAM) application guidelines. The ancestry inference accuracy of individuals from East Asian, European, African and mixed populations was verified. METHODS: DNA standard sample 9947A was used as the template to establish the optimal amplification conditions by adjusting primer balance, Mg²⁺ final concentration and optimizing PCR thermal cycle parameters and amplification volume. The allelic dropout, nonspecific amplification and whether the origin of the inferred samples matched the known information were compared to evaluate the performance of this system. RESULTS: The optimal dosage of this system was 0.125-2 ng DNA template. The results of InDel typing were accurate, the amplification equilibrium was good, and the species specificity was good. This system showed certain tolerance to DNA samples including the inhibitor such as hemoglobin (≤80 μmol/L), indigo (≤40 mmol/L), calcium ion (≤1.0 mmol/L), and humic acid (≤90 ng/μL). The system enabled the direct amplification of DNA from saliva and blood on filter paper, and the results of ethnic inference were accurate. The system successfully detected the mixed DNA sample from two individuals. The test results of the system for common biological materials in practical cases were accurate. CONCLUSIONS The results of the 38-plex InDel system are accurate and reliable, and the performance of the system meets the requirement of the SWGDAM guidelines. This system can accurately differentiate the ancestry origins of individuals from African, European, East Asian, and Eurasian populations and can be implemented in forensic practice.
Humans ; Polymorphism, Single Nucleotide ; Genotype ; Polymerase Chain Reaction ; DNA/genetics* ; DNA Fingerprinting/methods* ; INDEL Mutation ; Genetics, Population ; Gene Frequency