Glioblastoma multiforme (GBM) is the most aggressive grade IV astrocytoma which is the common type of malignant (cancerous) primary brain tumor.The median survival time was 15-17 months.At present, the conventional treatment methods of GBM are surgical resection, radiotherapy and temozolomide chemotherapy of Temozolomide.The invasiveness of tumor is not only the main obstacle of surgery, radiotherapy and chemotherapy, but also the main cause of death of GBM patients.GBM patients will gradually develop resistance to chemotherapy, leading to tumor regrowth or recurrence, but the exact mechanism is not very clear.In recent years, the role of hypoxia in tumor metastasis and invasion has been paid more and more attention.Understanding how hypoxia induces GBM cell invasiveness is particularly important for developing new and more effective therapies to combat this catastrophic disease.It is believed that hypoxia can increase the invasiveness of GBM by inducing degradation and remodeling of extracellular matrix, expression of tissue factors, promoting epithelial mesenchymal transition and angiogenesis, and regulating GBM ion channels.