【Objective】 To observe the effect of deglycerolized red blood cells suspended in MAP on preservation and explore the most effective preservation method. 【Methods】 Concentrated red blood cells were prepared by centrifuging 400 mL of whole blood on the third day after collection. 40% compound glycerol solution was added using the ACP 215 automatic blood cell analyzer, and the resulting mixture was stored in an ultra-low temperature refrigerator at -65℃ for 30 days. After thawing and washing, it was equally separated into two bags. The control group received 0.9% sodium chloride solution, while the experimental group received MAP. Both groups were stored at 2-6℃. Hematological parameters, hemolysis indexes and cell metabolism indexes were measured on day 0, 1, 3, 5, 7 and 14 after storage. The quality changes of both groups were observed during the 14-day storage period. 【Results】 The quality of red blood cells in both groups was assessed through a panel of quality tests, including volume, hemoglobin content, free hemoglobin content, white blood cell residue, glycerin residue and sterility. These results met the Quality Requirements outlined in the "Quality Requirements of Whole Blood and Component Blood" (GB18469-2012), Hematocrit, red blood cell count, Hb recovery rate after washing and MCV meet the detection limit outlined in the "Expert Consensus on Quality Evaluation Indicators for Frozen Red Blood Cells", and the residual amount of platelets exceeds the detection limit(≤1%). There were no significant differences in RBC, Hct, MCV and hemoglobin between the two groups during the 14 day storage period. The level of free hemoglobin, hemolysis rate and K⁺ value increased in both groups over time. Significant differences in free hemoglobin were found on day 3, 5, 7 and 14 between the two groups(P<0.05). Hemolysis rate was significantly different on days 3, 5, 7 and 14, while K⁺ value was significantly different only on day 14(P<0.05). On day 14, the osmotic fragility of red blood cells was higher in the control group than in the experimental group(P<0.05); The ATP and pH values of both groups decreased as storage time increased, and significant differences in ATP and pH value were found on day 3, 5, 7 and on day 1, 3, 5, 7 and 14, respectively(P<0.05). 【Conclusion】 Deglycerolized red blood cells suspended in MAP additive solution can extend the storage period of blood to 7 days. This study provides a reference for the formulation of relevant standards.

Gene-edited mice are the most ideal laboratory animals for studying human gene functions, exploring disease mechanisms, and developing new drugs. Strain resulting from low fertility, aging, illness, etc. can cause irreversible losses to scientific research, so strain rescues of genetically engineering mice require different measures accordingly. Meanwhile, cost control is another key point when a specific technology is applied. First of all, when the only remaining gene-edited mouse in reproductive age suddenly dies, the dead male mouse can be rescued by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), while the female mouse can be saved by ovarian transplantation, etc. Secondly, due to aging or diseases, mice can be saved through IVF-embryo transfer (ET) and unilateral epididymal tail assisted reproduction. Thirdly, round sperm injection (ROSI) and ovarian transplantation can be used to save endangered mice before sexual maturity with poor life status. This paper reviews rescue techniques of common endangered mice and their applications, which provides a reference for relevant practitioners to better maintain gene-edited mouse strains.

A hybrid method based on optimization and force-EMG relationships for predicting muscle force was presented and applied to the flexion movement of upper limb. The results were compared with those from classical optimization and from the method based on force-EMG relationships. The classical optimization can not predict the force of antagonistic muscles. The method based on force-EMG relationships can not satisfy the mechanical equilibrium. The hybrid method overcomes the two disadvantages, but the forces predicted by it fluctuate excessively. Now the hybrid method is just appropriate for use in predicting isometric and isokinetic movements.
Algorithms ; Electromyography ; methods ; Forecasting ; Humans ; Isometric Contraction ; physiology ; Models, Biological ; Muscle Contraction ; physiology ; Muscle, Skeletal ; physiology ; Physical Exertion ; physiology ; Stress, Mechanical

Finding the position of caput femoris center by exact and fast method is a key technique in total knee replacement navigation surgery. A method for finding the position of caput femoris center is described in this paper. The main idea is abstracting this problem to a geometrical model in which the center of a sphere can be easily worked out with a few known spherical points. Experiments show that the method is feasible and the precision satisfies the requirements of navigation surgery.
Algorithms ; Arthroplasty, Replacement, Knee ; instrumentation ; methods ; Biomechanical Phenomena ; Computer Simulation ; Humans ; Models, Biological ; Surgery, Computer-Assisted ; instrumentation ; methods

The design method of individual brace for adolescent idiopathic scoliosis (AIS) was proposed and its correction effect was investigated. Based on the 3D CT scanned data of a scoliosis patient, an accurate finite element model, which included thorax, lumbus, sacrum and pelvis, was developed; the construction of individual scoliosis brace was based on the T-L-S-P FE model, and the correction effects of three different strap tensions were investigated. The results showed that the individual brace treatment had better correction effect than did other braces in coronal plane, and it had no inverse effect on sagittal plane. The forces generated by the strap tensions are in agreement with results of the experiment reported in literature. The research provides an available method for the design of individual brace, which could help improve the effectiveness for correction of idiopathic scoliosis.
Adolescent ; Biomechanical Phenomena ; Braces ; Finite Element Analysis ; Humans ; Imaging, Three-Dimensional ; Male ; Scoliosis ; diagnostic imaging ; physiopathology ; therapy ; Spine ; diagnostic imaging ; physiopathology ; Stress, Mechanical ; Therapy, Computer-Assisted ; methods ; Tomography, X-Ray Computed

In this paper, we present an experimental research on the frameless registration of DSA/CT images based on frameless localization algorithm. The result shows that, 3D fusion and registration of vessels in the DSA images and anatomical structures in CT images will help surgeons to make accurate diagnosis and on plann operative.
Algorithms ; Angiography, Digital Subtraction ; methods ; Cerebral Angiography ; Humans ; Image Processing, Computer-Assisted ; methods ; Imaging, Three-Dimensional ; Tomography, X-Ray Computed ; methods

Nonalcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver dysfunction and has complex pathogenesis and progression. With in-depth studies in recent years, the theory of “two hits” has gradually been replaced by the theory of “multiple hits”. The theory of “multiple hits” including the neuroendocrine immune inflammatory network suggests that various factors participate in the progression of NAFLD, such as insulin resistance, adipose tissue dysfunction, mitochondrial dysfunction, endoplasmic reticulum stress, inflammatory activation, fatty acids, gut microbiota, iron overload, dietary factors, genetic factors, and epigenetic factors. With reference to the research advances in recent years, this article reviews the pathogenesis of NAFLD from the aspects of genetic and epigenetic factors, gut microbiota, intrahepatic immune cells, and aquaporin.

In vivo tumor imaging technique method based on bioluminescence principle was established to evaluate the anti-tumor effect of paclitaxel mixed micelle (PMM). MDA-MB-231 tumor cells with luciferase reporter vectors were firstly implanted into nude mice, and subsequently the luciferase substrate was regularly injected during intraperitoneal administration of PMM. Then the tumor size, growth and the intensity of light signals were monitored with in vivo imaging technique. The method of luciferase tumor in vivo imaging could be real-time, reliable and exact in labeling and reflecting the growth of tumors, and the observed results were consistent with that by conventional method, so it would be a feasible approach to study anti-tumor effect of drugs. The anti-tumor effect of paclitaxel mixed micelle was observed by this method, and the results showed that this formulation could inhibit growth of tumor, and the anti-tumor rate of it was about 85%.

Objective:To obtain highly purified recombinant human IGF-Ⅰ(rhIGF-Ⅰ) and identify it.Methods:rhIGF-Ⅰ Was purified through ion-exchange chromatography and gel filtration chromatography after the inclusion bodies of rhIGF-Ⅰ were extracted from Escherichia coli. The recombinant protein was characterized through molecular weight assay, Western-blot, and fluorescent chromatography. The renaturation and biological assay of rhIGF-Ⅰ were investigated. Results and Conclusions: The purity of rhIGF-Ⅰ was higher than 99%. The analysis of molecular weight, Western-blot, fluorescent chromatography and sequences of NH2-terminal 15 amino acids were same as those anticipated. 3-10 mg/ml was the concentration of renatured rhIGF-Ⅰ to support half-maximal stimulation of cell proliferation with BALB/c 3T3 cells.

In medical imaging field, doctors often complete the intra-subject registration of multi modality images by choosing a pair of anatomic landmarks. It is hard to choose the same landmark accurately in different imaging modality. For multi-modality image registration, mutual information measure is widely used because it suffers little from the difference among different modalities, but it has disadvantages of large amount of calculation and slow converges. In this paper, considering the convenience in practice, we firstly register different modality images by choosing a pair of corresponding landmarks. In order to decrease the error during choosing the landmarks, we further do the registration using mutual information method. The advantage of this solution is that the landmark based registration presents a good starting point for further mutual information (MI) registration, while the MI method decreases the difficulty of choosing landmarks. Experimental results showed that this registration solution was fast, accurate, and would have a good clinical potential application in the future.
Algorithms ; Diagnostic Imaging ; methods ; Humans ; Image Enhancement ; methods ; Image Interpretation, Computer-Assisted ; methods ; Skull ; anatomy & histology