Abstract: Objective　In order to provide reference for emergency treatment of a sudden food poisoning incident, pathogen detection and drug resistance analysis were carried out. Methods　Diarrheal stool and surplus food samples were detected by GB 4789 and the isolates were identified by VITEK2 and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), at the same time, the bacterial drug sensitivity test was carried out by using the method of microbroth dilution, and the isolates from different sources were molecularly classified by pulsed field gel electrophoresis (PFGE), and the correlation between the strains was analyzed by BioNumerics software. Results　Totaly 13 leftovers and 3 diarrhea patients were isolated and identified, The total number of colonies and coliforms in 7 leftovers samples all exceeded the standard, and Citrobacter freundii was detected in 5 leftovers and 2 stools. The results of drug sensitivity test showed that seven strains of Citrobacter freundii were sensitive to ciprofloxacin, tetracycline, chloramphenicol, gentamicin, amikacin, cefotaxime and meropenem, but completely resistant to ampicillin, and there was no multiple drug resistance. The results of pulsed field gel electrophoresis (PFGE) showed that 7 strains of Citrobacter freundii had the same PFGE bands and 100% homology, showing the same clone. Conclusions　This food poisoning incident was caused by Citrobacter freundii. The pathogen of food poisoning can be quickly and accurately determined by MALDI-TOF MS, which is beneficial to the early diagnosis and treatment of infectious diseases. It is suggested to strengthen the corresponding management, improve food safety awareness and prevent similar incidents.

Objective To analyze the complications of coronary rotational atherectomy and to evaluate the safety of the procedure. Methods We evaluated the procedural and angiographic outcomes of 248 consecutive procedures to rotational atherectomy between January 2000 and October 2016. Results 27 cases(10.9％)were found to have rotational atherectomy related complications. Among these 27 cases,coronary spasm occurred in 2 cases(0.8％),no reflow in 8 cases(3.2％), coronary dissection in 5 cases(2.0％),burr entrapment in 6 cases(2.4％),wire breakage in 3 cases(1.2％), and coronary perforation in 3 cases(1.2％). There were no death,acute myocardial infarction and emergent coronary bypass graft.14 case(5.6％)had PCI-related myocardial infarction during hospital stay.In-hospital major adverse cardiaccerebral event(MACCE)rate was 5.6％. Conclusions Coronary rotational atherectomy can be performed with high success rates and procedure-related complications are rare.

In vivo tumor imaging technique method based on bioluminescence principle was established to evaluate the anti-tumor effect of paclitaxel mixed micelle (PMM). MDA-MB-231 tumor cells with luciferase reporter vectors were firstly implanted into nude mice, and subsequently the luciferase substrate was regularly injected during intraperitoneal administration of PMM. Then the tumor size, growth and the intensity of light signals were monitored with in vivo imaging technique. The method of luciferase tumor in vivo imaging could be real-time, reliable and exact in labeling and reflecting the growth of tumors, and the observed results were consistent with that by conventional method, so it would be a feasible approach to study anti-tumor effect of drugs. The anti-tumor effect of paclitaxel mixed micelle was observed by this method, and the results showed that this formulation could inhibit growth of tumor, and the anti-tumor rate of it was about 85%.
Animals ; Antineoplastic Agents, Phytogenic ; administration & dosage ; pharmacology ; therapeutic use ; Breast Neoplasms ; drug therapy ; pathology ; Cell Line, Tumor ; Female ; Humans ; Luminescent Measurements ; Male ; Melanoma, Experimental ; drug therapy ; pathology ; Mice ; Mice, Inbred C57BL ; Mice, Nude ; Micelles ; Neoplasm Transplantation ; Paclitaxel ; administration & dosage ; pharmacology ; therapeutic use ; Particle Size ; Tumor Burden ; drug effects

Needle sticking method, which can be combined with multiple needling techniques, has been attached with great importance in recent years by doctors in clinic. Combining with the clinical experiences, the authors expounded the needle sticking method through its unified concept, differences between needle sticking method, which was an acupuncture technique, and stuck needle which was an accident during acupuncture, selection of needles, manipulations, mechanism of treatment, range of application, attentions and advantages of popularization. It is held that the technique can be widely applied for treatment of acute and chronic diseases of various departments with filiform needles. Easy to be manipulated, understood and mastered, the technique is without side effect and valuable to be popularized.
Acupuncture Therapy ; instrumentation ; methods ; Humans ; Medicine, Chinese Traditional

OBJECTIVEThe neuroprotective effect of erythropoietin (EPO) against 1-methyl-4-phenylpyridinium (MPP(+))-induced oxidative stress in cultured PC12 cells, as well as the underlying mechanism, were investigated.

METHODSPC12 cells impaired by MPP(+) were used as the cell model of Parkinson's disease. Methyl thiazolyl tetrazolium (MTT) was used to assay the viability of the PC12 cells exposed to gradient concentrations of EPO, and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay was used to analyze the apoptosis ratio of PC12 cells. The expression of Bcl-2 and Bax in PC12 cells were examined by Western blot, and the reactive oxygen species (ROS), the mitochondrial transmembrane potential and the activity of caspase-3 in each group were detected by spectrofluorometer.

RESULTSTreatment of PC12 cells with MPP(+) caused the loss of cell viability, which may be associated with the elevation in apoptotic rate, the formation of ROS and the disruption of mitochondrial transmembrane potential. It was also shown that MPP(+) significantly induced the upregulation of Bax/Bcl-2 ratio and the activation of caspase-3. In contrast, EPO significantly reversed these responses and had the maximum protective effect at 1 U/mL.

CONCLUSIONThe inhibitive effect of EPO on the MPP(+)-induced cytotoxicity may be ascribed to its anti-oxidative property and anti-apoptotic activity, and EPO may provide a useful therapeutic strategy for treatment of neurodegenerative diseases such as Parkinson's disease.

1-Methyl-4-phenylpyridinium ; toxicity ; Analysis of Variance ; Animals ; Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Cell Survival ; drug effects ; Dose-Response Relationship, Drug ; Drug Interactions ; Erythropoietin ; pharmacology ; Flow Cytometry ; methods ; Herbicides ; toxicity ; In Situ Nick-End Labeling ; methods ; Membrane Potential, Mitochondrial ; drug effects ; Neuroprotective Agents ; pharmacology ; PC12 Cells ; drug effects ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; Rats ; Reactive Oxygen Species ; metabolism ; Tetrazolium Salts ; Thiazoles ; bcl-2-Associated X Protein ; genetics ; metabolism

OBJECTIVESTo observe the effects of renal ischemic postconditioning (RI-Post) on myocardial apoptosis in rabbits with acute myocardial ischemia and reperfusion.

METHODSAll rabbits were subjected to 60 minutes ischemia by left anterior descending coronary artery occlusion (LADO) and 6 hours reperfusion. The rabbits are randomly divided into 3 groups (n = 8 in each group): (1) Ischemia-reperfusion (IR): LADO and reperfusion without additional intervention; (2) RI-Post: after 60 minutes of LADO, the left renal artery was occluded for 30 seconds and reperfused for 30 seconds and repeated 3 times, then the coronary artery was reperfused for 6 hours; (3) Medication intervention (MI): 10 minutes before coronary reperfusion, rabbits were treated with PKC antagonist GF109203X (0.05 mg/kg, IV), followed by RI-Post treatment and 6 hours coronary reperfusion. Myocardial apoptosis was measured by TUNEL and the myocardial Bcl-2 and Bax protein expressions were assessed by immunohistochemistry.

RESULTSCompared with the IR group and the MI group, myocardial apoptosis was significantly reduced (P < 0.05) and the Bcl-2 protein expression increased (P < 0.01) while the Bax protein expression decreased (P < 0.05) in the RI-Post group.

CONCLUSIONSRemote renal postconditioning applied right before the onset of coronary artery reperfusion can reduce the myocardial apoptosis induced by myocardial ischemia and reperfusion and up-regulate Bcl-2 while down-regulate Bax expression possibly by activation of protein kinase C.

Animals ; Apoptosis ; Female ; Ischemia ; metabolism ; Ischemic Preconditioning ; Kidney Diseases ; metabolism ; Male ; Myocardial Reperfusion Injury ; metabolism ; Myocardium ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Rabbits ; bcl-2-Associated X Protein ; metabolism

Objective To investigate the effect of overexpression of α 2,3-sialyltransferase (ST3Gal Ⅲ) on abilities of invasion in breast cancer cells MDA-MB-231.Methods ST3Gal Ⅲ gene lentiviral expression vector was transfected into MDA-MB-231 cells.Transfected cells were divided into 3 groups:blank group (Mock cells,M),control group (Parent cells P),experimental group (ST3Gal Ⅲ ST3).The expression of ST3Gal Ⅲ mRNA and protein were detected by Quantitative Real-time PCR(RT-PCR) and Western blot.The content of α2,3-sialic acids was analyzed by flow cytometry.The ability of migration and invasion in MDA-MB-231 cells were detected by cell adhesion assay and Transwell assay,respectively.Results The expression levels of ST3Gal Ⅲ mRNA and protein examined by RT-PCR and Western blot in control group and experimental group were 0.91 ± 0.04 and 1.85 ± 0.08 respectively.The expression levels in experimental group were 1.84-fold and 1.76-fold higher than control group(P ＜ 0.05).The amount of SLeX on the cell surface in experimental group was 92.86 ± 4.41 and was 1.61-fold higher than control group,which were significantly increased compared with that in the blank group and control group (P ＜ 0.05).OD570nm values of the adhesion assay in the in the blank group,the control group and the experimental group were 0.32 ± 0.02,0.29 ± 0.01 and 0.46 ±0.03,respectively,while those of the invasion assay were 0.93 ±0.02,0.81 ±0.01 and 1.46 ±0.13,and there was significant difference between the experimental group and the control group (P ＜ 0.05).Conclusion Overexpression of ST3Gal Ⅲ gene could enhance the invasion ability of MDA-MB-231 cells.

Objective To investigate the frequency of the breast cancer related lymphedema and its effects on the health outcomes of breast cancer survivors.Methods It was a cross-sectional study and 301 female breast cancer survivors were enrolled in the study.Circumference measurement were chosen to diagnose the lymphedema.Quality of life Questionnare-Core30 designed by European Organization for Research and Treatment of Cancer and Disabilities of Arm,Shoulder and Hand Scale were administered to assess the quality of life and upper limb function which were calculated as the main outcome variables.Results The incidence of the breast cancer related lymphedema was 15.0％.The patients with breast cancer related lymphedema had lower mean values in physical functioning and role functioning and the differences were statistically significant (P ＜0.05),respectively.Patients with breast cancer related lymphedema had higher DASH scores and the difference was statistically significant (P ＜ 0.05).Conclusions Breast cancer related lymphedema can not only decrease breast cancer survivors quality of life but also impair their upper limb function.It implied that breast cancer related lymphedema is a question which needed to be paid more attention.

Objective? To investigate the dietary sodium intake level of patients with heart failure and analyze its influencing factors, so as to provide reference for formulating dietary intervention strategies for patients with heart failure. Methods? From July 2018 to April 2019, 153 patients with heart failure in the First Affiliated Hospital of Nanjing Medical University were selected as the subject by convenient sampling. The General Questionnaire and Dietary Sodium Restriction Questionnaire(DSRQ) were collected, and 24-hour urine sodium determination was used to assess the patient's dietary sodium intake level. Results? A total of 155 questionnaires were distributed and 153 questionnaires were returned. The average dietary sodium intake of 153 patients with heart failure was (3 725.39±1 741.84) mg/d. The dietary sodium intake of 24 patients was within the recommended range (2 000-3 000 mg/d), accounting for 15.7%(24/153). The total score of the DSRQ was (54.83±9.15), and the scores of attitude and subjective normative dimension, cognitive dimension and perceptual behavior control were (23.24±3.15), (11.77±2.08), and (19.82±6.13), respectively.Univariate analysis showed different genders, tastes, dental health status, and whether the dietary sodium intake was different for patients prescribed a low-sodium diet, the differences were statistically significant (P＜ 0.05);correlation analysis showed that appetite score and sodium intake in patients with heart failure was correlated, the difference was statistically significant (r=0.174, P=0.031), and the sodium-stable diet behavioral control score was associated with sodium intake in patients with heart failure (r=-0.329, P＜ 0.001). Multiple linear regression analysis showed that the patient's gender, taste, and patient's perceptual behavioral control of sodium-restricted diet were the main influencing factors of dietary sodium intake in patients with heart failure, explaining 21.5% of the total variation in dietary sodium intake. Conclusions? The dietary sodium intake of patients with heart failure is higher than the recommended amount of the guideline, and the current situation is not optimistic. In particular, health care workers should pay attention to individualized dietary guidance and interventions in men with low salty taste and low sodium-stable dietary behavioral scores to reduce heart failure and readmission rates.

Objective To investigate the length of hospital stay,hospitalization expense and mortality attributable to the occurrence of carbapenem-resistant Enterobacterales(CRE)infection in patients in intensive care unit(ICU).Methods Patients admitted to the ICU in a tertiary first-class hospital from 2017 to 2022 were selected for the study.According to whether CRE infection occurred,patients were divided into infected group and non-infected group.Propensity score matching method was used to conduct a 1∶1 match between the infected group and non-infected group.Length of hospital stay,hospitalization expense and mortality of patients after matching were analyzed sta-tistically.A generalized linear model was established to recalculate the odds ratio(OR)of length of hospital stay,hospitalization expense and mortality of patients after matching.Results After propensity score matching,length of hospital stay of patients in the infected group extended by 10.56 days(P＜0.001),hospitalization expense in-creased by 36 021.02 Yuan(P＜0.001),and mortality increased by 6.70％(P=0.035).The results of the gene-ralized linear model indicated that OR for length of hospital stay,hospitalization expense,and mortality were 1.187(95％CI:1.013-1.393),1.134(95％CI:0.975-1.318),and 1.130(95％CI:1.049-1.218)respectively for CRE infected patients,compared with non-infected patients,except for hospitalization expense,length of hospital stay and mortality between two groups were statistically significant(both P＜0.05).Conclusion CRE infection in ICU patients will increase the length of hospital stay,economic burden,and mortality of patients.Measures should be taken to prevent and control CRE infection.