1.Preliminary Study of Medical Device Adverse Events
Hui TANG ; Hongtao SHANG ; Wen LIU
Chinese Medical Equipment Journal 2004;0(08):-
Medical devices are widely used in the treatment of diseases,health care and rehabilitation process as modern science and technology products,and become an important means in the field of modern medicine. How to ensure that medical devices are used safe and effective is the common problem in production,management,use and monitoring.
2.Amyloidoma of neck: report of a case.
Wen-Ting HUANG ; Shang-Mei LIU
Chinese Journal of Pathology 2007;36(2):138-138
Adult
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Amyloidosis
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pathology
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surgery
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Diagnosis, Differential
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Female
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Humans
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Muscular Diseases
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pathology
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surgery
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Neck Muscles
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pathology
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surgery
4.Effect of estradiol on cholesterol metabolism in J774a.1 mouse mononuclear/macrophage cells.
Xue WANG ; Jun LIU ; Wen-Li DUAN ; Jing SHANG
Acta Pharmaceutica Sinica 2014;49(7):1013-1018
To explore the anti-atherosclerotic mechanism of estrogen and especially observe the effect of estradiol on the content of cholesterol in J774a.1 mouse mononuclear/macrophage-derived foam cells which were incubated with oxidized low-density lipoproteins (ox-LDL). J774a.1 mouse mononuclear/macrophages were incubated with ox-LDL or with both ox-LDL and estradiol (1, 0.1 or 0.01 micromol x L(-1)). Oil red O staining was used to observe the formation of foam cells, and cholesterol oxidase fluorometric was used to determine the content of cellular cholesterol content. Western blotting and RTFQ-PCR were used to observe the expressions of scavenger receptor class B type I (SR-B I ) in J774a.1 foam cells. Compared with the control cells, J774a.1 mouse mononuclear/macrophage-derived foam cells showed significantly increased contents of total cholesterol and cholesterol ester (P < 0.001) and decreased SR-B I mRNA expression (P < 0.01). Estradiol treatment significantly lowered the contents of total cholesterol and cholesterol ester (P < 0.05), and increased SR-B I protein and mRNA expression (P < 0.01) in the foam cells in a dose-dependent manner. Estradiol can inhibit the formation of mononuclear/macrophage-derived foam cells by decreasing the contents of total cholesterol and cholesterol ester and up-regulating the expression of SR-B I in the foam cells.
Animals
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Cell Line
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Cholesterol
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metabolism
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Cholesterol Esters
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metabolism
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Estradiol
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pharmacology
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Foam Cells
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cytology
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metabolism
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Lipoproteins, LDL
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metabolism
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Macrophages
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drug effects
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metabolism
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Mice
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Scavenger Receptors, Class B
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metabolism
5.Right leg mass with thrombocytopenia.
Wen-xiu YANG ; Li BAO ; Qi-lan LIU ; Shang-yi LIU
Chinese Journal of Pathology 2011;40(3):191-192
Antigens, CD20
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metabolism
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Female
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Follow-Up Studies
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Humans
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Interferon Regulatory Factors
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metabolism
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Leg
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pathology
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Lymphoma, Large B-Cell, Diffuse
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complications
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metabolism
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pathology
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surgery
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Middle Aged
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Skin Neoplasms
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complications
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metabolism
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pathology
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surgery
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Thrombocytopenia
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complications
6.Clinical study on "Jin's three-needling" in treatment of generalized anxiety disorder.
Wen-zheng LUO ; Hai-jing LIU ; Shang-ying MEI
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(3):201-203
OBJECTIVETo study the clinical effect of "Jin's three-needling" in the treatment of generalized anxiety disorder.
METHODSFifty-eight patients with generalized anxiety were randomly assigned to two groups equally, the medication group treated with anti-anxiety drugs and the acupuncture group with "Jin's three-needling". The treatment course was 6 weeks. The clinical effects were evaluated with Hamilton anxiety scale (HAMA), clinical global impression (CGI), and treatment emergent symptom scale (TESS) before treatment and at the end of 2nd, 4th, 6th week of the treatment course. The concentration of 5-hydroxytryptamine (5-HT) in platelet, and plasma levels of corticosterone (CS) and adrenocorticotropic hormone (ACTH) were measured with high performance liquid chromatography-electrochemical detection (HPLC-ED) method before and after treatment.
RESULTSThe clinical effects in the two groups were equivalent, while the adverse reaction found in the acupuncture group was less than that in the medication group (P < 0.05). The platelet concentration of 5-HT and plasma ACTH level decreased significantly in both groups after treatment with insignificant difference between the group (P < 0.05). The plasma CS level had no obvious change in the two groups after treatment as compared with that before treatment respectively.
CONCLUSION"Jin's three-needling" shows similar curative effect on generalized anxiety to routine Western medicine but with less adverse reaction, which may be realized through regulating the platelet 5-HT concentration and plasma ACTH level.
Acupuncture Therapy ; methods ; Adolescent ; Adrenocorticotropic Hormone ; blood ; Adult ; Anxiety Disorders ; blood ; psychology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Psychiatric Status Rating Scales ; Serotonin ; blood
7.Survey of radiation levels in an abnormally high radon hot spring
Bing SHANG ; Hongxing CUI ; Xiangmin WEN ; Zhikai LIN ; Xiaohua FU ; Yunyun WU ; Jianxiang LIU
Chinese Journal of Radiological Medicine and Protection 2011;31(6):698-702
Objective To investigate the radiation level around Jiangzha hot spring,and to analyze the sources of pollution.Methods The radon and its progeny concentration,γ dose rate in hot spring living district and surrounding area were measured with ATD monitors,radon and WL continuous measurement devices,γ dose rate meter.Results The radon concentration in water was 23 -764 Bq/L.Radon concentration indoors,outdoor and in bathing place were 254 -876 799,688 -709 and 3590-15 299 Bq/m3,respectively.γ dose rate were 205 -28718 nGy/h indoor,4104- 18254 n Gy/h outdoors.Conclusions Jiangzha hot spring is an area with rare high radon and high nature radiation.Its radiation level and health effects are worthy for further attention.
8.Detection of circulating tumor cells from peripheral blood in patients with esophageal squamous cell carcinoma and its clinical significance
Jun LI ; Wei WANG ; Yuanyuan QIAO ; Dajin ZHANG ; Feng WEN ; Liqun SHANG ; Xuechang LI ; Junqiang LIU
Chinese Journal of Thoracic and Cardiovascular Surgery 2012;28(3):157-160
ObjectiveTo explore the relationship between circulating tumor cells(CTCs) in the peripheral blood of patients with esophageal squamous cell carcinoma(ESCC) and the physiopathological characteristics of esophageal neoplasms.MethodsUsing negative selection system,we depleted red blood cells(RBCs) in red blood cell lysis buffer,depleted white blood cells (WBCs) with Miltenyi magnetic beads and enriched the rare cells from ESCC patients'peripheral blood.Immunofluorence staining (IF) was adopted to identify CTCs.ResultsCirculating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma was closely related to cell differentiation grade,the invasion of primary cancer,lymph node status,P-TNM stages,and was rarely related to the sex,age or the location of tumor.ConclusionThe results suggest that circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma may express the development of esophageal cancer and may be served as a tumor marker to evaluate the biological behavior of esophageal cancer.
9.Surgical therapy for bronchobiliary fistula due to hepatic cystic echinococcosis
Jingtao ZHOU ; Arji TUERGANAILI ; Yingmei SHAO ; Gang LI ; Jia LIU ; Shaohua SHANG ; Hao WEN
Chinese Journal of General Surgery 2013;28(11):839-841
Objective To explore the diagnosis and treatment efficacy of bronchobiliary fistula due to hepatic cystic echinococcosis.Methods A retrospective evaluation of 39 patients with BBF was performed during 1992 to 2012.We divided the 39 patients into group A treated before 2001 and group B after 2002.A retrospective analysis was made.Results There were three deaths among the 39 BBF patients due to hepatic hydatid disease.The cause of death was septic shock due to severe infection.There were no statistical differences in the basic factors,age (t =0.84,P =0.554),gender (P =1.0),and sputum volume (t =0.98,P =0.703),hydatid diameter (t =1.11,P =0.406),operation time,chest infection (P =1.0),mortality (P =0.235) between the two groups (P > 0.05).While postoperative length of stay(t =7.64,P =0.000),postoperative complications of residual cavity (P =0.001),length of tube drainage(t =6.747,P =0.01),recurrence of bronchial fistula (P =0.022),pleural dissemination (P =0.018),reoperation rate (P =0.049) were all in favour of group B (P < 0.05).Conclusions Surgery is the choice of therapy for BBF due to hepatic hydatid disease,and one-stage procedure is expected to achieve the best outcomes.
10.Study on the Effect of Safflower Injection on the Regulation of Genes related to Qi-deficiency and Blood Stasis Syndrome in Rats
Lingfei LIANG ; Xiaohu ZHAI ; Yuwen LI ; Tianlong LIU ; Yikai ZHANG ; Peijin SHANG ; Aidong WEN
Progress in Modern Biomedicine 2017;17(22):4217-4222
Objective:To establish two differential gene expression profiles of qi-deficiency and blood stasis syndrome before or after safflower injection treatment by using gene chip technology;compared and analyzed to ensure the effective genes that are responsible for the therapeutic effects of safflower injection against qi-deficiency and blood stasis syndrome in rats.Furthermore,speculated the effect mechanism of the therapeutic genes.Methods:Fifteen SD rats were randomly divided into 3 groups (n=5):control group,model group,and medication group.Qi-deficiency and blood stasis model was established by subjecting the rats to hunger and fatigue for two weeks.After a week of the modeling,safflower injection (100 mg/kg/d) was administered daily via the tail vein for 7 days in medication group,and the rats in model group were injected with saline of the same volume.Control group received normal feeding.At the end of the experiment,rats were killed and whole blood was collected to evaluate the blood stream change and extract mRNAs in blood samples.Qualified mRNAs were reverse transcribed into cDNA which was then used in gene chip hybridization.The genes regulated by safflower injection were determined by the fluorescence signal and the functional mechanisms of safflower injection were confirmed by further querying genealogy databases and reviewing literatures.Results:After two weeks of the modeling,the whole blood viscosity under various shear rates was significantly increased in the model rats which showed faint,blood stasis and weight loss,indicating that the model is made successfully.The increased whole blood viscosity and qi-deficiency and blood stasis syndrome were obviously reversed by safflower injection treatment.Compared with the control group,252 genes up-regulated while 54 genes down-regulated in model group;compared with the model group,196 genes up-regulated while 32 genes down-regulated.Among these,16 differentially expressed genes were involved in inflammation and immune response.Conclusions:Safflower injection was effective in treating qi deficiency and blood stasis syndrome,which was achieved by regulating inflammation related genes.