BACKGROUND: Schwann cells are the only glial cells in the peripheral nervous system and play an important role in the regeneration of peripheral nerves, while they have poor proliferation capacity and decreased activity, need allografts, and are difficult to culture in vitro. OBJECTIVE: To analyze the feasibility of repairing neurologic defects with tissue-engineered nerves constructed with Schwann cells derived from bone marrow stromal stem cells. DESIGN, TIME AND SETTING: Randomized controlled observation was conducted at Heilongiiang Institute of Veterinary Pharmaceutics between March 2004 and April 2005.MATERIALS: Twenty-four 8-weck-old female Wistar rats were used to establish animal models with 10 nun defect of sciatic nerve. METHODS: Twenty-four rats were divided into 3 groups by random digits table, tissue-engineered nerve group, polylactic acid robe group and autologons nerve group, with 8 rats in each group. Tissue-engineered nerve group: tissue-engineered nerve Was used to bridge neurologic defect, which was constructed with Schwann cells derived from bone marrow stromal stem cells, natural extracelhilar matrix gel and degradable polylactic acid tube. Polylactic acid robe group: injecting extracellular matrix gel into degradable polylactic acid tube to bridge neurologic defect. Autologous nerve group: 10 mm of nerve was cut and performed end-to-end anastomosis after revolving 180 degrees. MAIN OUTCOME MEASURES: Functional recovery of sciatic nerve was detected with electrophysiological observation of nerve, and histological observation and axon count of the newly generated nerve tissue at 12 weeks after transplantation. RESULTS: After introduction, adult bone marrow stromal stem cells had the morphology and properties of Schwann cells. The regenerated nerve had grown to the distal end passing through the defect at 12 weeks after transplantation. The detection indexes in the tissue-engineered nerve group and autologous nerve group were better than that in the polylactic acid tube group (P < 0.05), there were no significant differences between the tissue-engineered nerve group and autoiogous nerve group (P < 0.05); The degradation and absorption of polylactic acid tube were obvious in the tissue-engineered nerve group and autologous nerve group. CONCLUSION: Human bone marrow stromal stem cells can be induced to differentiate into Schwann cells in vitro, and tissue-engineered nerve constructed with Schwann cells, extracellular matrix and degradable polylactic acid tube can repair peripheral neurologic defect.

Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb Methods Micellar electrokinetic capillary chromatography (MECC) with a buffer solution of 5 mmol/L H 3BO 3 NaOH containing 20 mmol/L of sodium deoxycholate (SDC) (pH=10) was used for their separation at a voltage of 17 kv Results The four active constituents were completely separated within 5 min Conclusion This method was found to be simple and rapid and gave satisfactory results

This paper argues on the connotation of information ethics,issues related to personal privacy and professional ethics in hospital library service,contents of information ethical education,construction of hospital library ethics,construction of domestic and overseas hospital libraries.

Information ethics brings new issues and reflections on medical information services under network environment.By analyzing relevant issues including equally and freely acquiring medical information service,network knowledge property,network privacy protection,and information monopoly,this article explores the ethical construction of medical information staff.

Objective To investigate the effects of hypertonic saline/hetastarch solution (HHS) on stress hormones and glucose metabolism in hemorrhagic shock rabbit.Methods Fourteen rabbits of both sexes weighing 2.2-2.6 kg were randomly divided into 2 groups : HHS group ( n = 7) and lactated Ringer's solution (LRS) group ( n = 7). The animals were anesthetized with intravenous 20% urethane 5 ml? kg-1 . Femoral artery was cannulated for BP monitoring and femoral vein was cannulated for removal of blood and fluid infusion. Hemorrhagic shock was induced according to Wiggers. MAP was maintained at 45 mm Hg for 45 min. Then the animals in HHS group received HHS 6 ml? kg-1 and those in LRS group LRS 6 ml? kg-1 . Venous blood samples were taken before shock (baseline), during shock before resuscitation, and 30, 60, 120 min after fluid resuscitation for determination of plasma epinephrine, glucagon, insulin and blood glucose concentration. The insulin sensitivity index (ISI) was calculated.Results After resuscitation MAP returned to baseline level in HHS group while in LRS group MAP was still lower than the baseline. The plasma epinephrine, glucagon and blood glucose concentration increased significantly while plasma insulin concentration decreased significantly during shock before fluid resuscitation compared to the baseline in both groups. After fluid resuscitation plasma epinephrine and glucagon concentration decreased significantly and plasma insulin concentration increased significantly in HHS group whereas in LRS group plasma epinephrine, glucagon and insulin concentration kept increasing. The blood glucose level was significantly lower at 60 and 120 min after resuscitation in HHS group than in LRS group. ISI was decreased after resuscitation in both groups but was significantly lower at 60 and 120 min after resuscitation in LRS group than in HHS group.Conclusion Resuscitation with HHS can reduce the stress response and ameliorate the decrease in insulin sensitivity during hemorrhagic shock.

Brain ; pathology ; Central Nervous System Diseases ; congenital ; diagnosis ; pathology ; physiopathology ; Diagnosis, Differential ; Humans ; Infant, Newborn ; Magnetic Resonance Imaging ; Male ; Mobius Syndrome ; diagnosis ; pathology ; physiopathology

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Objective To evaluate the effect of diets rich in Ruditapes philippinarum on the development of atherosclerosis. Methods The rabbits were fed with normal forage or high lipid diet with a daily supplement of Ruditapes philippinarum for 8 weeks respectively. Serum lipid, the area of atherosclerotic plaque and IMT were analysed.Results Compared with control group, serum lipid profiles didn't change obviously and atherosclerotic lesion was not observed in group fed with a supplement of RP. Compared with model group, the concentration of serum TC, TG and LDL C/HDL C decreased significantly, while the concentration of serum HDL C increased significantly in group fed with high lipid forage and a supplement of RP (p

[Objective]To determine the biological effects of transfection of adeno-associated rirus(AAV) BMP4/7 on rabbit bone marrow stromal cells (BMSCs).[Method]The mature peptide of BMP4 and BMP7 were gained by One-Step RT-PCR from the human placenta and the BMP4/7 fusion gene was gained through gene recombinant techniques and then transferred to pGEM plasmid. The BMP4/7 fusion genen was cut down from the pGEM-BMP4/7 and the recombination was successfully completed in colibacillus, and recombinant adeno-assosiated was produced in 293 cells. Rabbit BMSCs were transfected with the recombinant adeno-assosiated virus vectors carrying BMP4/7 fusion gene with differen multiplicitv of infection(MOI) values. Cell growth curves were drawn to evaluate the biologic effect of AAV-BMP4/7 on cyto-activity. The transfection efficiency was measured using MTT method. The ossification of cells was evaluated by investigating the shape change of the cell ability of ALP and OC at 7,14 days after transfection. Cells were then transfected with AAV-BMP4/7 and AAV-EGFP,respectively.[Result]1.We successfully constructed the recombinant adeno-assosiated virus with BMP4/7 fusion gene.2.The ttransfection efficiency of AAV-BMP4/7 was roughly 72% without siginifficant biologic effect on cyto-activity.The ossification of cells was significant after transfection with AAV-BMP4/7. The 1?105 vg/cell MOI value of transfection efficiency was stroger than 5~104 vg/cell MOI value (59.3,8%). 3. The concentrations of ALP and OC in AAV-BMP4/7 transfection groups were significantbly higher than in AAV-EGFP groups (tALP=896.88 P

Acute respiratory distress syndrome (ARDS) patients experiencing protective mechanical ventilation, is associated with a marked mortality reduction. However, the incidence of acute cor pulmonale (ACP) in ARDS patients has recently been reported to range between 22% and 25%, as well as a trend for higher mortality. Therefore, the mechanical ventilation strategy is proposed, not only based on the protection of the lung, but also focused on the impact on the right ventricle function. Currently, point-of-care ultrasound has been widely practiced in a variety of clinical setting, which plays more and more important role in the early detection and management of ARDS and its complications. A retrospective study concerning the incidence, pathophysiology and risk factors for ACP patients in ARDS was done to analysis the application of lung ultrasound and echocardiography combined with lung ultrasound in clinical hemodynamics monitoring, and so as to optimize the ventilation setting to protect the function of lung and right ventricle. Further exploration of effective improvement of the pulmonary vascular and right ventricle function the goal-directed ultrasound approach, and the diagnosis and treatment flow is expected.